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2005 | 54 | 4 |

Tytuł artykułu

Susceptibility testing and resistance phenotypes detection in bacterial pathogens using the VITEK 2 system

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A set of well characterized strains, collected in Polish hospitals, including Gram-negative (n = 93) and Gram-positive (n = 90) isolates was used in the study. The VITEK 2 AST-cards were used in the analysis according to the manufacturer's recommendations. Comparison of the susceptibility data obtained by the standard method and by VITEK 2 cards proved concordant in 99% of cases. Clinically important mechanisms were revealed by the VITEK 2 AES with > 95% agreement with reference data including methicillin resistance in staphylococci (98%), high-level aminoglycoside resistance in enterococci (100%), VanA and VanB phenotypes in enterococci (100%), and ESBLs in Enterobacteriaccae (93.8%). The VITEK 2 AES System appears a reliable tool for the detection and interpretive reading of clinically important mechanisms of resistance and can be recommended for routine work.

Wydawca

-

Rocznik

Tom

54

Numer

4

Opis fizyczny

p.311-316,ref.

Twórcy

autor
  • National Institute of Public Health, Chelmska 30/34, 00-725 Warsaw, Poland
autor

Bibliografia

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  • Blondel-Hill E., C. Hetchler, D. Andrews and L. Lapointe. 2003. Evaluation of Vitek 2 to analysis of Entero-bacteriaceae using the Advanced Expert System (AES) versus interpretative susceptibility guidelines used at Dynacare Kasper Medical Laboratories, Edmonton, Alberta. Clin. Microbiol. Infect. 9: 1091-103.
  • Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing; Fifteenth informational supplement: M100-S15. CLSI, Villanova, PA.
  • Dahl K.H., G. Skov Simonsen, R. Olsvik and A. Sundsfjord. 1999. Heterogeneity in vanB gene cluster of genetically diverse clinical strains of vancomycin-resistant enterococci. Antimicrob. Agents Chemother. 43: 1105-1110.
  • Dutka-Malen S., S. Evers and P. Courvalin. 1995. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J. Clin. Microbiol. 33: 24-27.
  • Facklam R. and M.D. Collins. 1989. Identification of Enterococcus species isolated from human infections by a conventional test scheme. J Clin. Microbiol. 27: 731-734
  • Fiett J., A. Pałucha, B. Miączyńska, M. Stankiewicz, H. Przondo-Mordarska, W. Hryniewicz and M. Gniadkowski. 2000. A novel complex mutant beta-lactamase, TEM-68, identified in a Klebsiella pneumoniae isolate from an outbreak of extended-spectrum beta-lactamase-producing klebsiellae. Antimicrob. Agents Chemother. 44: 1499-1505.
  • Horstkotte M.A., J.K.M. Knobloch., H. Rohde, S. Dobinsky and D. Mack. 2002. Rapid detection of methicillin resistance in coagulase-negative staphylococci with the VITEK 2 system. J. Clin. Microbiol. 40: 3291-3295.
  • Jarlier V, J.E. Nicolas, G. Fournier and A. Philippon. 1988. Extended broad-spectrum β-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev. Infect. Dis. 10: 2141-2143.
  • Ligozzi M., C. Bernini, M.G. Bonora, M. de Fatima, J. Zuliani and R. Fontana. 2002. Evaluation of the Vitek 2 system for identification and antimicrobial susceptibility testing of medically relevant Gram-positive cocci. J. Clin. Microbiol. 40: 1681-1686.
  • Livermore D.M. 1995. β-lactamases in laboratory and clinical resistance. Clin. Microbiol. Rev. 8: 557-58.
  • Leverstein-van Hall M.A., A.C. Fluit, A. Paauw, A.T.A. Box, S. Brisse and J. Verhoef. 2002. Evaluation of the Etest ESBL and the BD Phoenix, VITEK 1, and VITEK 2 automated instruments for detection of extended-spectrum β-lactamases in multiresistant Escherichia coli and Klebsiella spp. J. Clin. Microbiol. 40: 3703-3711.
  • Murakami K., W. Minimide, K. Wada, E. Nakamura, H. Teraoka and S. Watanabe. 1991. Identification of methicillin resistant strains of Staphylococcus aureus by polymerase chain reaction. J. Clin. Microbiol. 29: 2240-2244.
  • National Committee for Clinical Laboratory Standards. 2003a. Methods for Dilution Susceptibility Tests for Bacteria That Grow Aerobically; Approved standard - Sixth Edition: M7-A6. NCCLS, Villanova, PA.
  • National Committee for Clinical Laboratory Standards. 2003b. Performance Standards for Antimicrobial Disk Susceptibility Testing; Approved Standard - Eighth Edition M2-A8. NCCLS, Villanova, PA.
  • Pałucha A., B. Mikiewicz, W. Hryniewicz and M. Gniadkowski. 1999. Concurrent outbreaks of extended-spectrum beta-lactamase-producing organisms of the family Enterobacteriaceae in a Warsaw hospital. J. Antimicrob. Chemother. 44: 489-499.
  • Ruoff K.L., R.A. Whiley and D. Beighton. 1999. Streptococcus, pp. 283-305. In: PR. Murray, E.J. Baron, M.A. Pfaller, Tenover FC, Yolken RH. (eds): Manual of Clinical Microbiology, ed. 7. Washington, American Society for Microbiology
  • Karlovsky J.A., M.K. Weaver, C. Thornsberry, M.J. Dowzicky, M.E. Jones and D.F. Sahm. 2003. Comparison of four antimicrobial susceptibility testing methods to determine the in vitro activities of piperacillin and piperacillin-tazobactam against clinical isolates of Enterbacteriaceae and Pseudomonas aeruginosa. J. Clin. Microbiol. 41: 3339-3343.
  • Stefaniuk E„ A. Baraniak, M. Gniadkowski and W. Hryniewicz. 2003. The evaluation of the BD PHOENIX™ automated identification and susceptibility testing system in clinical microbiology laboratory practice. Eur. J. Clin. Microbiol. Infect. Dis. 22: 479-485.

Typ dokumentu

Bibliografia

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Identyfikator YADDA

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