Syntetyczny plytkowy czynnik aktywujacy [PAF] jako skladnik rozcienczalnika dla nasienia knura

• Autorzy: Kordan W , Strzezek J.

Warianty tytułu

EN

Synthetic platelet activating factor [PAF] as a boar semen extender component

• Języki publikacji: PL

Abstrakty

EN

The aim of this study was to investigate the effects of PAF addition on selected motility characteristics and plasmalemma integrity of boar spermatozoa following liquid storage in a boar semen extender, Kortowo-3 (K-3), supplemented with lipoprotein fractions extracted from hen egg yolk (LPFh) or lyophilized lipoprotein fractions extracted from ostrich egg yolk (LPFo), at 5°C and 16°C. Sperm motility was evaluated using a computer system (CASA). The determination of AspAT activity in sperm extracts as well as fluorescent analysis, with a fluorochrome, Hoechst 33258, were used to assess the plasmalemma integrity overlying the middle-piece and acrosomal regions of spermatozoa, respectively. It was confirmed that the addition of exogenous PAF to K-3 extender containing LPFh or LPFo had a beneficial effect on the sperm quality parameters during storage at 5°C or 16°C. This phenomenon was manifested by an increase in motility and survivability of spermatozoa. The use of LPFh or LPFo as a component of boar semen extender had a protective effect on the plasmalemma integrity overlying the middle-piece and acrosomal regions of PAF-treated spermatozoa.

Słowa kluczowe

PL

rozcienczalniki; knury; konserwacja; trzoda chlewna; ruchliwosc plemnikow; integralnosc blon komorkowych; plytkowy czynnik aktywujacy; jakosc; nasienie; koncentracja plemnikow

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 05
• Opis fizyczny: s.560-562,rys.,tab.,bibliogr.

Twórcy

autor

Kordan W

• Uniwersytet Warminsko-Mazurski, ul.Oczapowskiego 5, 10-718 Olsztyn

autor

Strzezek J.

Bibliografia

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