Presentation of beta-galactosidase activity secreted by plant cells

• Autorzy: Stano J , Micieta K. , Grancai D. , Blanarikova V.

Warianty tytułu

PL

Prezentacja aktywnosci beta-galaktozydazy wydzielanej przez komorki roslinne

• Języki publikacji: EN

Abstrakty

EN

Extracellular plant β-galactosidase from opium poppy and celandine was detected on agar plates by the presence of dyed zones from 1-naphthyl-β-D-galactopyranoside used as a synthetic substrate. Evaluating the azo-dye zones assessed the degree β-galactosidase activity. No coloration of the agar medium was observed on non-inoculated parts, in medium inoculated with heat – inactivated cells (10 min. at 100oC) or in medium without substrate with all calli tested. On the agar plates with substrate and sterile opium poppy, celandine or gherkin seedlings (2–6 days old) changes in coloration were observed showing the release of β-galactosidase from the roots during germination.

PL

Autorzy badali aktywność b-galaktozydazy wydzielanej przez komórki roślinne maku polnego i glistnika jaskółcze ziele na agarze metodą oceny zabarwionych stref z 1-naftylo-b- D-galaktopiranozydem użytym jako syntetyczny substrat. Zmiany zabarwienia wskazują na zanikanie b-galaktozydazy z korzeni podczas procesu kiełkowania.

Słowa kluczowe

EN

plant cell; enzyme activity; beta-galactosidase; enzyme secretion; celandine; opium poppy; seedling; callus culture

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2007
• Tom: 53
• Numer: 1
• Opis fizyczny: p.27-33,ref.

Twórcy

autor

Stano J

• Comenius University, Odbojarov 0, 832 32 Bratislava 3, Slovakia

autor

Micieta K.

autor

Grancai D.

autor

Blanarikova V.

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