Synthesis of 5-IASA-UDP-GlcNAc and its use for the photoaffinity labeling of a novel UDP-GlcNAc pyrophosphorylase

• Autorzy: Szumilo H. , Szumilo T. , Elbein A.D.
• Języki publikacji: EN

Abstrakty

EN

Photoreactivable 5-l3-(p-iodoazidosalicylamide)allylJ-UDP-GlcNAc (5-IASA-UDP- -GlcNAc) was synthesized by a four-step procedure and used for photoaffinity labeling of UDP-GlcNAc-dependent enzymes. Upon iodination with 12SI, the compound was successfully applied to probe a purified UDP-GlcNAc pyrophos­phorylase from pig liver. The enzyme was photoinactivated by the probe in the concentration-dependent manner, and was protected by UDP-GlcNAc and, to a lesser extent, by UTP and UDP-GlcCOOH.

Słowa kluczowe

EN

pig; N-hydroxysuccinimidyl-4-azidosalicylic acid; 5-[3-[p-iodoazidosalicylamide]allyl]; N-acetylglucosamine; liver; pyrophosphorylation; thin-layer chromatography

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 1
• Opis fizyczny: p.55-59,fig.

Twórcy

autor

Szumilo H.

• Medical School, S.Staszica 6, 20-081 Lublin, Poland

autor

Szumilo T.

autor

Elbein A.D.

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