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2003 | 08 | 2 |

Tytuł artykułu

The genetic characteristics Saccharomyces cerevisiae aciplus mutants

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A series of 30 Saccharomyces cerevisiae aci+ mutants (characterized as acidifying Ogur's glucose medium containing bromocresol purple) were isolated after EMS mutagenesis. All the mutants excreted acid metabolites to the medium after 24 or 48 hours of incubation. The character of the aci+ mutations was defined using classical genetic techniques. Three of the aci+ mutants were studied by molecular genetics techniques.

Wydawca

-

Rocznik

Tom

08

Numer

2

Opis fizyczny

p.261-268,fig.

Twórcy

  • University of Zielona Gora, Monte Cassino 21b, 65-001 Zielona Gora, Poland
autor
autor

Bibliografia

  • 1.Goffeau, A. Four years of post-genomic life with 6000 yeast genes. FEBS Lett. 480 (2000) 37-41.
  • 2.Goncar, M., Lachowicz, T., Sybirny, A. and Witkowska, R. Genetics, physiology and biochemistry of some Saccharomyces cerevisiae mutants excreting acidic products. Genetics of Respiratory Enzymes in Yeast, Karpacz, 1990, 53.
  • 3.Boniewska-Bernacka, E., Lachowicz, T.M. and Kotylak, Z. Genetical and biochemical characterization of cycle Krebs mutant in yeast. Acta Microb. Polon. 47 (1998) 131-140.
  • 4.Grochowalska, R., Machnicka, B., Wysocki, R. and Lachowicz, T.M. Identyfication of some genes responsible for aci+ phenotype in yeast Saccharomyces cerevisiae. Cell. Mol. Biol. Lett. 7 (2002) 280.
  • 5.Ogur, M., Cocker, L. and Ogur, S. Glutamate auxotrophs in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 14 (1964) 193.
  • 6.Ogur, M., Roshanmanesh, A. and Ogur, S. Tricarboxylic acid cycle mutants in Saccharomyces cerevisiae. Comparison of independently derived mutants. Science 147 (1965) 1590.
  • 7.Kim, K.S., Rosenkrantz, M.S. and Guarente, L. Saccharomyces cerevisiae contains two functional citrate synthase genes. Mol. Cell. Biol. 6 (1986) 1936-1942.
  • 8.McAlister-Henn, L. and Thompson, L.M. Isolation and expression of the gene encoding yeast mitochondrial malate dehydrogenase. J. Bacteriol. 169 (1987) 5157-5166.
  • 9.Wu, M. and Tzagoloff, A. Mitochondrial and cytoplasmic fumarases in Saccharomyces cerevisiae are encoded by a single nuclear gene FUM1. J. Biol. Chem. 262 (1987) 12275-12282.
  • 10.Repetto, B. and Tzagoloff, A. Structure and regulation of KGD1, the structural gene for the yeast α-ketoglutarate dehydrogenase. Mol. Cell. Biol. 9 (1989) 2695-2705.
  • 11.Repetto, B. and Tzagoloff, A. Structure and regulation of KGD2, the structural gene for the yeast dihydrolipoyl transuccinylase. Mol. Cell. Biol. 10 (1990) 4221-4232.
  • 12.Gangloff, S.P., Margueret, D. and Lauquin, G.J-M. Molecular cloning of the yeast mitochondrial aconitase gene (ACO1) and evidence of a synergistic regulation of expression by glucose plus glutamate. Mol. Cell. Biol. 10 (1990) 3551-3561.
  • 13.Minad, K.I. and McAlister-Henn, L. Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenases. Mol. Cell. Biol. 11 (1991) 370-380.
  • 14.Kaclikova, E., Lachowicz, T.M., Gbelska, Y. and Subik, J. Fumaric acid over production in yeast mutants deficient in fumarase. FEMS Microb. Lett. 91 (1992) 101-106.
  • 15.Cupp, J.R. and McAlister-Henn, L. Kinetic analysis of NAD+-isocitrate dehydrogenase with altered isocitrate binding sites: contribution of IDH1 and IDH2 subunits to regulation and catalysis. Biochemistry 32 (1993) 9323-9328.
  • 16.Przybyla-Zawislak, B., Dennis, R.A., Zakharkin, S.O. and McCommon, M.T. Genes of succinyl-CoA ligase from Saccharomyces cerevisiae. Eur. J. Biochem. 248 (1998) 736-743.
  • 17.Przybyla-Zawislak, B., Gadde, D.M., Ducharme, K. and McCommon, M.T. Genetic and biochemical interactions involving Tricarboxylic Acid Cycle (TCA) function using a collection of mutants defective in all TCA cycle genes. Genetics 152 (1999) 153-166.
  • 18.Boniewska, E., Wysocki, R., Grochowalska, R., Machnicka, B., Ułaszewski, S. and Lachowicz, T.M. Isolation of three novel genes of the yeast Saccharomyces cerevisiae complementing aci mutation responsible for medium acidification. Yeast 18 (2001) 216.
  • 19.Hanahan D. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166 (1983) 557-580.
  • 20.Sambrook, J., Fritsch, E.F. and Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).
  • 21.Fink, G. Metabolism of amino acids and amines. Ethylmetane sulfonate (EMS) mutagenesis. Methods Enzymol. 17 (1976) 71-73.
  • 22.Sherman, F., Fink, G.R. and Hicks, J.B. Methods in Yeast Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1987).
  • 23.Ito, H., Fukuda, H., Murata, K. and Kimura, A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153 (1983) 163-168.
  • 24.Kaiser, C., Michaelis, S. and Mitchel, A. Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1994).
  • 25.Altschul, S.F., Gish, W., Miller, W., Myers, W. and Lipman, J. Basic local aligment search tool. J. Mol. Biol. 215 (1990) 403-410.
  • 26.Baker-Brachmann C., Davies A., Cost G.J., Caputo E., Li J., Hieter P. and Boeke J.D. Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14 (1998) 115-132.
  • 27.Bonneaud, N., Ozier-Kalogeropoulos, O., Li, G., Labouesse, M., Minvielle- Sebastia, L. and Lacroute, F. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors, Yeast 7 (1991)609-615.
  • 28.Machnicka, B., Grochowalska, R., Lipihski, T., Boniewska, E., Słomińska, L. and Lachowicz, T.M. An identification of the products excreted by Saccharomyces cerevisiae aci+ mutants (acidifying growth media). Cell. Mol. Biol. Lett. 7 (2002) 304.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-087a405d-9c85-4faf-a6c3-959e31a61cad
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