Chromatographic properties of the cadmium induced SOD isoform in Rhizopogon roseolus

• Autorzy: Miszalski Z , Niewiadomska E. , Skoczowski A. , Botton B.
• Języki publikacji: EN

Abstrakty

EN

Exposure of Rhizopogon roseolus mycelia to 15 mmol·dm⁻³ cadmium for 24 h induces a different pattern of Mn-SOD on polyacrylamide gels, probably being a changed form of an originally existing one. The presence of cadmium affects the chromatographic properties of this enzyme and its mobility through the acrylamide gel. This new isoform was purified using DEAE Trisacryl chromatography. Cadmium induced isoform adsorbed stronger to the ligands and was eluated with a Tris-HCl buffer containing 0.1 mol·dm⁻³ NaCl. SOD from control samples (not treated with cadmium) was eluated with the same buffer without NaCl.

Słowa kluczowe

EN

superoxide dismutase; Rhizopogon roseolus; chromatography; cadmium

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1998
• Tom: 20
• Numer: 3
• Opis fizyczny: p.229-233,fig.

Twórcy

autor

Miszalski Z

• Polish Academy of Sciences, 31-016 Cracow, Poland

autor

Niewiadomska E.

autor

Skoczowski A.

autor

Botton B.

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