pH-dependent influence of a quaternary ammonium salt and an aminoester on the yeast Saccharomyces cerevisiae ultrastructure

• Autorzy: Oblak E , Adamski R. , Lachowicz T.M.
• Języki publikacji: EN

Abstrakty

EN

Quaternary ammonium salts inhibited the growth of yeast especially at pH higher (pH 8) than optimal. It was postulated that compounds integrate with the cell membrane and interfere with its functions. The yeast cell ultrastructure investigated under an electron microscope confirms this hypothesis. A relatively high percentage of cells treated at pH 6 with the quaternary ammonium salt of alanine derivative (DMALM-12) at the minimal inhibitory concentration showed an irregularity in the cell shape. No such irregularity was observed in the control. Besides, in the cells treated with the drug, practically no lipid droplets were seen at all. Inside the control cells, electron-dense round bodies were clearly seen and interpreted as vacuoles. These bodies were absent in the cells treated with DMALM-12. Although the yeast cells growing at pH 8 showed a more or less normal shape, they seemed to have difficulty in budding - no fully developed buds were found in the preparations. Only some convexities of the cell wall were seen that could be the beginning of budding which stopped early after the start. Some changes in the round bodies interpreted as vacuoles were visible: they were less dense and full of granules.

Słowa kluczowe

EN

yeast; cell membrane; ammonium salt; microbiology; pH; yeast cell; ultrastructure; Saccharomyces cerevisiae; aminoester

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 1
• Opis fizyczny: p.105-110,fig.

Twórcy

autor

Oblak E

• University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

autor

Adamski R.

autor

Lachowicz T.M.

Bibliografia

• 1.Lachowicz T.M., Obłąk E. and Piątkowski J. Auxotrophy - stimulated sensitivity to quaternary ammonium salts and its relation to active transport in yeast. Bul. Pol. Acad. Sci. 40 (1992) 173-182.
• 2.Obłąk E., Ułaszewski S., Morawiecki A., Witek S., Witkowska R., Majcher K. and Lachowicz T.M. Quaternary ammonium salt resistant mutants in yeast Saccharomyces cerevisiae. Yeast 5 (Special Issue) (1989) 273-278.
• 3.Obłąk E., Ułaszewski S. and Lachowicz T.M. Mutants of Saccharomyces cerevisiae resistant to a quaternary ammonium salt. Acta Microbiol. Polon. 37 (1988) 261-269.
• 4.Lachowicz T.M., Witkowska R. and Obłąk E. Amino acid auxotrophy increases sensitivity of Saccharomyces cerevisiae to a quaternary ammonium salt IM. Acta Microbiol. Polon. 39 (1990) 157-162.
• 5.Obłąk E., Bącal J. and Lachowicz T.M. A quaternary ammonium salt as an inhibitor of plasma membrane H+-ATPase in yeast Saccharomyces cerevisiae. Cell. Mol. Biol. Lett. 5 (2000) 315-324.
• 6.Obłąk E., Lachowicz T.M., Łuczyński J. and Witek S. Comparative studies of biological activities of the lysosomotropic aminoesters and quaternary ammonium salts on yeast Saccharomyces cerevisiae. Cell. Mol. Biol. Lett. 6 (2001) 871-880.
• 7.Obłąk E., Lachowicz T.M. and Witek S. DL - leucine transport in a Saccharomyces cerevisiae mutant resistant to quaternary ammonium salts. Folia Microbiol. 44 (1996) 116-119.
• 8.Thompson R. Amphiphilic glycine-based esters as soft antimicrobial agents, PhD Thesis, Goeteborg 1992.
• 9.Obłąk E., Lachowicz T.M., Łuczyński J. and Witek S. Lysosomotropic N,N- dimethyl α-aminoacid n-alkylesters and their quaternary ammonium salts as plasma membrane and mitochondrial ATPases inhibitors. Cell. Mol. Biol. Lett. 7 (2002) 1121-1129.
• 10.Reynolds E.W. The use of lead citrate at high pH as an electron - opaque stain electron microscopy. J. Cell. Biol. 17 (1963) 208-212.