Effects of adenosine receptor agonists and antagonists on cytochrome P450 extinction in the liver of rats in the course of acute pancreatitis

• Autorzy: Celinski K , Madro A. , Czechowska G. , Slomka M. , Prozorow-Krol B. , Biskup W. , Malm A. , Juda M. , Wielosz M.
• Języki publikacji: EN

Abstrakty

EN

In the course of acute pancreatitis the liver is an organ that is especially exposed to damage. The presence of adenosine receptors was observed in the whole digestive system. The aim of the experiment was to define the correlation between the extinction of cytochrome P450 in the liver of rats and adenosine receptor agonists and antagonists in the course of necrotizing acute pancreatitis. The experiments were carried out on Wistar male rats weighing 250 g. Acute pancreatitis was induced injecting 5% sodium taurocholate to the biliary-pancreatic duct. Prior to the induction of acute pancreatitis the animals were injected intraperitoneally with selective agonists and antagonists: CGS 21680 (selective A2 agonist), 3 mg/kg, ZM 241385 (selective A2a antagonist), 3 mg/kg, DPCPX (A1 antagonist), 1 mg/kg, 1.3-Dipropyl-8-phenylxantine (selective A1 antagonist), 3 mg/kg, IB-MECA (A3 agonist), 0.75 mg/kg. The determinations were performed in hepatic microsomes obtained according to Guegenrichs method Cytochrome P450 extinction was determined by Matsubars technique. The results obtained reveal statistically significantly decreased cytochrome P450 extinction after sodium taurocholate administration. Decreased levels of extinction were also observed after combined administration of sodium taurocholate + Phenylxantine and sodium taurocholate + ZM. The level of IB-MECA remained unchanged in comparison to the controls. However DPCPX and CGS administration increased the extinction of cytochrome P450. The diverse influence of adenosine receptor agonists and antagonists used in the experiment on cytochrom P450 extinction seems to modify the course of the inflammatory process after using 5% sodium taurocholate.

Słowa kluczowe

EN

acute pancreatitis; rat; liver; cytochrome P450; adenosine receptor agonist; adenosine receptor antagonist

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 05
• Opis fizyczny: p.528-531,ref.

Twórcy

autor

Celinski K

• Medical University, Jaczewski Street 8, 20-954 Lublin, Poland

autor

Madro A.

autor

Czechowska G.

autor

Slomka M.

autor

Prozorow-Krol B.

autor

Biskup W.

autor

Malm A.

autor

Juda M.

autor

Wielosz M.

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