Distribution of atropine and scopolamine in different organs and stages of development in Datura stramonium L. [Solanaceae]. Structure and ultrastructure of biosynthesizing cells

• Autorzy: Iranbakhsh A , Oshaghi M.A. , Majd A.
• Języki publikacji: EN

Abstrakty

EN

This paper reports a study of the alkaloid content of different parts of Datura stramonium L. (Solanaceae) in various stages of its growth and development, and the location, structure and ultrastructure of alkaloid-biosynthesizing cells (idioblasts) in different parts of naturally grown and cultured plant material. TLC, HPLC, and GC/MS methods were used for alkaloid assays. The results showed that alkaloid production starts from the end of the second week after seed germination, increases in different organs up to the tenth week of growth, and then decreases. Leaves and capsules showed the highest alkaloid content in the vegetative and generative stages, respectively. In leaves the alkaloids decreased rapidly in the generative stage. The highest alkaloid content was recorded in vegetative leaves, followed in descending order by vegetative petioles, generative and vegetative stems, generative petioles, generative roots, generative leaves, vegetative roots and mature seeds. The organs as well as calli derived from different leaf parts were examined for the presence of idioblasts by microscopic and cytochemical methods. Idioblasts were present only in semi-hyaline callus originated from leaf base; they were spherical or oval, with a thick cell wall and large central vacuole. These observations should prove helpful in attempts to produce specific alkaloids in naturally grown plants and cell cultures.

Słowa kluczowe

EN

scopolamine; atropine; alkaloid content; structure; development; Solanaceae; Datura stramonium; distribution; ultrastructure; plant material

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2006
• Tom: 48
• Numer: 1
• Opis fizyczny: p.13-18,fig.,ref.

Twórcy

autor

Iranbakhsh A

• Islamic Azad University, Garmsar Branch, Garmsar, 35815/144, I.R., Iran

autor

Oshaghi M.A.

autor

Majd A.

Bibliografia

• Afsharypuor S, Mostajeran A, and Mokhtary R. 1995. Variation of scopolamine and atropine in different parts of Datura metel during development. Planta Medica 61: 383-384.
• Constabel F. 1983. Protoplast technology applied to metabolite production. International Review of Cytology Suppl. 16: 209-217.
• Dechaux C, and Boitel-Conti M. 2005. A strategy for overaccumulation of scopolamine in Datura innoxia hairy root cultures. Acta Biologica Cracoviensia Series Botanica 47: 101-107.
• Duez P, Chamart S, Hanocq J, Molle L, Vanhaele M, and Vanhaelen-Fastre R. 1985. Comparison between thin-layer chromatography-densitometry and high-performance liquid chromatography for the determination of hyoscyamine and hyoscyne in leaves, fruit and seeds of Datura (Datura spp.). Journal of Chromatography 329: 415-442.
• Evans WC, and Major VA. 1986. The alkaloids of the genus Datura, section Brugmansia. Part V. Alkaloids of D. sanguinea R. and P. and related esters of tropane -3α, 6β, 7β-triol. Journal of the Chemical Society. Perkin Transactions 1: 2775-2778.
• Evans WC. 1989. Pharmacognosy, 13th ed. Baillière Tindall, London.
• Hartmann T, Witte L, Oprach G, and Toppel G. 1986. Reinvestigation of the alkaloid composition of Atropa belladonna plants, root cultures, and cell suspension cultures. Planta Medica 52: 390-395.
• Hayat MA. 2000. Principles and techniques of electron microscopy: Biological applications. 4th ed. Cambridge University Press, Cambridge.
• Ionkova I, Witte L. and Alfermann H-A. 1994. Spectrum of tropane alkaloids in transformed roots of Datura innoxia and Hyoscyamus x gyorffii cultivated In vitro. Planta Medica 60: 182-184.
• Kitamura Y, Sato M, and Miura H. 1992. Differences of atropine esterase activity between intact roots and cultured roots of various tropane alkaloid-producing plants. Phytochemistry 31: 1191-1194.
• Klan ZF. 1931. Influence of period of vegetation and development of plant on the alkaloidal content of Hyoscyamus niger L. Journal of American Pharmaceutical Association 20: 1163-1175.
• Lanoue A, Boitel-Conti M, Dechaux C, Laberche J-C, Christen P, and Sangwan-Norreel B. 2004. Comparison of growth properties, alkaloid production and water uptake of two selected Datura hairy root lines. Acta Biologica Cracoviensia Series Botanica 46: 185-192.
• Lindsey K, and Yeoman MM. 1983. The relationship between growth rate, differentiation and alkaloid accumulation in cell cultures. Journal of Experimental Botany 34: 1055-1065.
• Mersey B, and Culter AG. 1986. Differential distribution of specific indole alkaloids in leaves of Catharanthus roseus. Canadian Journal of Botany 64: 1039-1045.
• Miraldi E, Masti A, Ferri S, and Comparini IB. 2001. Distribution of hyoscyamine and scopolamine in Datura stramonium. Fitoterapia 72: 644-648.
• Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physlologia Plantarum 15: 473-497.
• Oshima T, Sagara K, Tong Y, Zhan GG, and Chen Y. 1989. Application of Ion-Pair High Performance Liquid Chromatography for analysis of hyoscyamine and scopolamine in solanaceous crude drugs. Chemical and Pharmaceutical Bulletin 37: 2456-2458.
• Parr AJ, Payne J, Eagles J, Chapman BT, Robins RJ, and Rhodes MJC. 1990. Variation in tropane alkaloid accumulation within the solanaceas and strategies for its exploitation. Phytochemistry 29: 2545-2550.
• Papadoyannis IN. 1995. Determination of Datura alkaloids by using chromatographic techniques: A review. Natural Toxins 3: 310-316.
• Payne J, Hamill JD, Robins RJ, and Rhodes MJC. 1987. Production of hyoscyamine by "Hairy Root" cultures of Datura stramonium. Planta Medica. 53: 474-478.
• Robins RJ, Parr AJ, Bent EG, and Rhodes MJC. 1991. Studies on the biosynthesis of tropane alkaloids in Datura stramonium L. transformed root cultures. Planta 183: 185-195.
• Sato F, Hashimoto T, Hachiya A, Tamura K, Choi KB, Morishige T, et al., 2001. Metabolic engineering of plant alkaloid biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 98: 367-372.
• Witte L, Müller K, and Alfermann H-A. 1987. Investigation of the alkaloid pattern of Datura innoxia plants by capillary gas-liquid-chromatography-mass spectrometry. Planta Medica 53: 192-197.
• Woo HS, Park JM, and Yang JW. 1997. Induction of branched roots by cutting method in Hyoscyamus niger root culture. Plant Cell Tissue and Organ Culture 48: 131-134.