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Czasopismo

2008 | 54 | 1 |

Tytuł artykułu

Przeglad metod stosowanych w analizie antyoksydacyjnych wyciagow roslinnych

Autorzy

Warianty tytułu

EN
Review of the methods applied to measuring of antioxidant activity of plant extracts

Języki publikacji

PL

Abstrakty

PL
Wolne rodniki są wysoce reaktywnymi i niebezpiecznymi cząstkami, którym ostatnio przypisuje się kluczową rolę w patogenezie tzw. chorób cywilizacyjnych. Ich wszechobecność powoduje, że żyjemy w chronicznym stresie oksydacyjnym, który jest przyczyną przeciążenia i nieskuteczności naturalnych systemów obronnych organizmów. Wtórne metabolity roślinne obecne w diecie i ekstraktach z roślin leczniczych często wykazują silne właściwości antyoksydacyjne. Istnieje szereg metod pomiaru tej aktywności. Najbardziej rozpowszechnione jest stosowanie tzw. metod in vitro (np. DPPH, ABT S, F-C, DMPD). Artykuł dokonuje przeglądu tych metod oraz zwraca uwagę na potrzebę ich standaryzacji.
EN
Free radicals are highly reactive and dangerous species that can cause a wide spectrum of cell damage. The organisms possess an antioxidant defense which helps to keep oxidative- antioxidative balance. Chronic oxidative stress is responsible for some diseases (e.g. diabetes, arteriosclerosis) development. A great number of medicinal plants contain compounds which are potential antioxidants. Free radical scavenging activity of plant extracts is determined by using different techniques. The most common are in vitro methods (e.g. DPPH, ABT S, F-C, DMPD) but in vivo assays are also known. Although an increasing interest in the studies on antioxidant activity is observed, there is no standardization of most analytical methods.

Wydawca

-

Czasopismo

Rocznik

Tom

54

Numer

1

Opis fizyczny

s.68-78,bibliogr.

Twórcy

autor
  • Akademia Medyczna, ul.Chodzki 1, 20-093 Lublin
autor

Bibliografia

  • 1. B all S. Antyoksydanty w medycynie i zdrowiu człowieka. Warszawa 2001.
  • 2. Mello L, Hernandez S, Marrazza G, Mascini M, Tatsuo Kubota L. Investigations of the antioxidant properties of plant extracts using a DNA-electrochemical biosensor. Biosens Bioelectron 2005; 21:1374-82.
  • 3. L ee HS. HPLC analysis of phenolic compounds. W: L.M. Nollet (red.) Food analysis by HPLC. New York 2000.
  • 4. Grajek W. Przeciwutleniacze w żywności. Warszawa 2007.
  • 5. A rts MJ, Haenen GR, Voss HP, Bast A. Antioxidant capacity of reaction products limits the applicability of the Trolox Equivalent Antioxidant Capacity (TEA C) assay. Food Chem Toxicol 2004; 42:45-9.
  • 6. Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. J Sci Technol 2004; 26:211-19.
  • 7. B artoń HJ, Fołta M, Zachwieja Z. Zastosowanie metod FRAP, ABT S, i DPPH w badaniu aktywności antyoksydacyjnej produktów spożywczych. Nowiny Lek 2005; 74:510-13.
  • 8. B artoń HJ, Fołta M. New approach in the analysis of antioxidant activity of coloured biological samples: a modification of the method of DPPH radical scavenging. W: Molecular and Physiological Aspects of the Regulatory Processes of the Organism. Proceeding of the XV International Symposium of the PolishNetwork of Molecular and Cellular Biology UNESCO/PAN. Kraków 2006.
  • 9. O zyurt D, Demirata B, Apak R. Determination of total antioxidant capacity by a new spectrophotometric method based on Ce(IV) reducing capacity measurement. Talanta 2006; 71:115-65.
  • 10. A rnao MB. Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case. Trends Food Sci Technol 2000; 11:419-21.
  • 11. Nenadis N, Tsimidou M. Observations on the estimation of scavenging activity of phenolic compounds using rapid 1,1-diphenyl-2-picrylhydrazyl (DPPH•) tests. J Am Chem Soc 2002; 79: 1191-5.
  • 12. Sanchez-Moreno C. Methods used to evaluate the free radical scavenging activity in food and biological systems. Food Sci Technol Int 2002; 8:121–37.
  • 13. O sman AM, Wong KKY , Fernyhough A. ABT S radical-driven oxidation of polyphenols: Isolation and structural elucidation of covalent adducts. Biochem Biophys Res Commun 2006; 346:321-9.
  • 14. E rel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABT S radical cation. Clin Biochem 2004; 37:277-85.
  • 15. Mariken JT, Arts J, Dallinga S, Voss HP, Haenen G, Bast A. A new approach to assess the total antioxidant capacity using the TEA C assay. Food Chem 2004; 88:567-70.
  • 16. Zhang Q, Zhang J, Shen J, Silva A, Dennis DA, Barrow CJ. A simple 96-well microplate method for estimation of total polyphenol content in seaweeds. J Appl Physiol 2006; 18:445–50.
  • 17. Y u Z, Dahlgren RA. Evaluation of methods for measuring polyphenols in conifer foliage. Analyst 2000; 26:98-113.
  • 18. Sadler NP, Jacobs H. Application of the Folin-Ciocalteau reagent to the determination of salbutamol in pharmaceutical preparations. Talanta 1995, 42:1385-8.
  • 19. Fernández-Pachón MS., Villaño D, García-Parrilla MC. Antioxidant activity of wines and relation with their polyphenolic composition. Anal Chim Acta 2004, 513:113-18.
  • 20. A l-Abachi MQ, Haddi H, Al-Abachi AM. Spectrofotometric determination of amoxicilin by reaction with N,Ndimethyl- p-phenylenediamine and potassium hexacyanoferrate (III). Anal Chim Acta 2005, 554:184-9.
  • 21. Firuzi O, Lacanna A, Petrucci R, Marrosu G, Saso L. Evaluation of the antioxidant activity of flavonoids by “ferric reducing antioxidant power” assay and cyclic voltammetry. Biochim Biophys Acta 2005,1721:174-84.
  • 22. B enzie IFF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a measure of ”antioxidant power”: The FRAP assay. Anal Biochem 1996, 239:70-6.
  • 23. A pak R, Guclu K, Ozyurek M, Karademir SE, Altun M. Novel total antioxidant capacity index for dietary polyphenols and vitamin C and E, using their cupric ion reducing capability in the presence of neocuproine: The CUPRAC method. J Agric Food Chem 2004, 52:7970-81.
  • 24. A pak R, Guclu K, Ozyurek M, Karademir SE, Altun M. Total antioxidant capacity assay of human serum using copper(II)-neocuproine as chromogenic oxidant: The CUPRAC method. Free Radic Res 2005; 39:949-61.
  • 25. Price JA, Sanny ChG, Shevlin D. Application of manual assessment of oxygen radical absorbent capacity (ORAC) for use in high throughput assay of “total” antioxidant activity of drugs and natural products. J Pharmacol Toxicol Methods 2006; 54:56-61.
  • 26. Huang D, Ou B, Prior R. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005; 53:1841-56.
  • 27. Prior RL, Cao G. In vivo total antioxidant capacity: Comparison of different analytical methods. Free Radic Biol Med 1999; 27:1173-81.
  • 28. Caldwell C, Charles R. A device for the semiautomatic determination of oxygen-radical absorbance capacity. Anal Biochem 2000, 287:226-33.
  • 29. Ninfali P, Bacchiocca M, Biagotti E, Servili M, Montedoro G. Validation of the Oxygen Radical Absorbance Capacity(ORAC) parameter as a new index of quality and stability of virgin olive oil. J Am Chem Soc2002, 79:977-82.
  • 30. Ghiselli A, Serafini M, Maiani G, Azzini E, Ferro-Luzzi A. A fluorescence-based method for measuring total plasma antioxidant capability. Free Radic Biol Med 1995;18:29-36.
  • 31. O rdoudi SA, Tsimidou MZ. Crocin bleaching assay (CBA ) in stucture-radical scavenging activity studies of selected phenolic compounds. J Agric Food Chem 2006, 54 9347-56. 32. L ichtenthäler R, Marx F, Kind OM. Determination of antioxidative capacities using an enhanced totaloxidant scavenging capacity (TO SC) assay. Eur Food Res Technol 2003; 216:166–73.
  • 33. Atanassova D, Kefalas P, Psillakis E. Measuring the antioxidant activity of olive oil mill Wastewater usingchemiluminescence. Analyst 2002; 127:183-98.
  • 34. Moure A, Cruz JM, Franco D, Dominguez JM, Siniero J, Nunez MJ, Parajo JC. Natural antioxidants from residual sources. Food Chem 2001; 72:145-71.
  • 35. Niki E. Antioxidant activity: Are we measuring it correctly? Nutrition 2002; 18:524-5.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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