Usefulness of PCR method for detection of Leishmania in Poland

• Autorzy: Myjak P , Szulta J. , de Almeida M.E. , da Silva A.J. , Steurer F. , Lass A. , Pietkiewicz H. , Nahorski W.L. , Goljan J. , Knap J. , Szostakowska B.
• Języki publikacji: EN

Abstrakty

EN

Leishmania parasites are the etiological agents of leishmaniosis, with severe course and often fatal prognosis, and the global number of cases has increased in recent decades. The gold standards for the diagnosis of leishmaniosis are microscopic examinations and culture in vitro of the different clinical specimens. The sensitivity of these methods is insufficient. Recent development in specific and sensitive molecular methods (PCR) allows for detection as well as identification of the parasite species (subspecies). The aim of the study was to estimate the usefulness of molecular methods (PCR) for detection of Leishmania species and consequently for the implementation of such methods in routine diagnostics of leishmaniosis in Polish patients returning from endemic areas of the disease. In our investigations we used 54 known Leishmania positive DNA templates (from culture and clinical specimens) received from the CDC (Atlanta, GA, USA). Moreover, 25 samples of bone marrow, blood or other tissues obtained from 18 Polish individuals suspected of leishmaniosis were also examined. In PCR we used two pairs of primers specific to the conserved region of Leishmania kinetoplast DNA (kDNA) minicircle (13A/13B and F/R). Using these primers we obtained amplicons in all DNA templates from the CDC and in three Polish patients suspected for Leishmania infection. In one sample from among these cases we also obtained positive results with DNA isolated from a blood specimen which was previously negative in microscopic examinations.

Słowa kluczowe

EN

polymerase chain reaction; leishmaniasis; parasite species; Poland; Leishmania; protozoan species; molecular diagnosis; detection; human disease; parasitic infection

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2009
• Tom: 58
• Numer: 3
• Opis fizyczny: p.219-222,ref.

Twórcy

autor

Myjak P

• Medical University of Gdansk, Powstania Styczniowego 9b, 81-519 Gdynia, Poland

autor

Szulta J.

autor

de Almeida M.E.

autor

da Silva A.J.

autor

Steurer F.

autor

Lass A.

autor

Pietkiewicz H.

autor

Nahorski W.L.

autor

Goljan J.

autor

Knap J.

autor

Szostakowska B.

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