Rapid DNA extraction for screening soil filamentous fungi using PCR amplification

• Autorzy: Plaza G A , Upchurch R. , Brigmon R.L. , Whitman W.B. , Ulfig K.
• Języki publikacji: EN

Abstrakty

EN

A simple and rapid procedure for efficiently isolating fungi DNA suitable for use as a template for PCR amplification and other molecular assays is described. The main advantages of the method are: (1) the mycelium is directly recovered from Petri-dish cultures; (2) the technique is rapid and relatively easy to perform , and (3) it allows for processing of around 50 samples during a single day; (4) it is inexpensive; (5) the quality and quantity of DNA obtained are suitable for molecular assays; (6) it can be applied to filamentous fungi from soil as well as from a fungi from other environmental sources; and (7) it does not require the use of expensive and specialized equipment or hazardous reagents.

Słowa kluczowe

EN

soil fungi; DNA isolation; filamentous fungi; polymerase chain reaction

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2004
• Tom: 13
• Numer: 3
• Opis fizyczny: p.315-318,fig.,ref.

Twórcy

autor

Plaza G A

• Institute for Ecology of Industrial Areas, Kossutha 6, 40-844 Katowice, Poland

autor

Upchurch R.

autor

Brigmon R.L.

autor

Whitman W.B.

autor

Ulfig K.

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