Construction of stable anionic liposome-plasmid particles using the heating method: a preliminary investigation

• Autorzy: Mozafari M R , Reed C.J. , Rostron C. , Kocum C. , Piskin E.
• Języki publikacji: EN

Abstrakty

EN

Recent advances in liposome technology have resulted in the production of effective drug delivery formulations, although toxicity concerns remain. In order to overcome this problem we prepared anionie liposomes without using any volatile organic solvent or detergent. Liposomes prepared by this heating method (HM-liposomes) were characterised in terms of morphology, stability and DNA incorporation efficiency. Scanning tunnelling microscopy (STM) and optical microscopy were used to study the morphological characteristics and size distribution of HM-liposomes. Microscopic studies revealed formation of spherical bilayered structures with stabilities of at least eight months and also enabled measuring the diameter and the bilayer thickness of the vesicles. Plasmid DNA encapsulation efficiencies of up to 70.3% were determined for HM-liposomes.

Słowa kluczowe

EN

liposome; anionic liposome; scanning tunneling microscopy; plasmid DNA; heating method

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 3
• Opis fizyczny: p.923-927,fig.

Twórcy

autor

Mozafari M R

• Liverpool John Moores University, Liverpool, L3 3AF, England, U.K.

autor

Reed C.J.

autor

Rostron C.

autor

Kocum C.

autor

Piskin E.

Bibliografia

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