Detection of bovine leucocyte adhesion deficiency [BLAD] carriers using a new PCR test

• Autorzy: Kaminski S , Czarnik U
• Języki publikacji: EN

Abstrakty

EN

In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non-random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.

Słowa kluczowe

EN

cattle; bovine leucocyte adhesion deficiency; polymerase chain reaction; bacterial infection

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 1
• Opis fizyczny: p.51-55,fig.

Twórcy

autor

Kaminski S

• Department of Animal Genetics, University of Agriculture and Technology, M.Oczapowskiego 5-030, 10-718 Olsztyn, Poland

autor

Czarnik U

• Department of Animal Genetics, University of Agriculture and Technology, M.Oczapowskiego 5-030, 10-718 Olsztyn, Poland

Bibliografia

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