Different routes of infection with Encephalitozoon intestinalis affect the development of immune response in immunocompetent and immunodeficient mice

• Autorzy: Salat J , Kopecky J. , Janeckova B. , Ditrich O.
• Języki publikacji: EN

Abstrakty

EN

The development of an immune response of immunocompetent BALB/c and C57BL/6 mice with different levels of resistance to microsporidial infection and immunodeficient SCID mice was analysed in relation to the route of infection with Encephalitozoon intestinalis. Cell-mediated immune responses were characterised by measurement of IFN-γ and quantification of CD4+, CD8+ and NK cells. Intraperitoneal (i.p.) infection induced higher levels of IFN-γ in ex vivo cultures of peritoneal exudate cells or splenocytes than peroral (p.o.) infection. Numbers of CD4+ and CD8+ T lymphocytes in the spleen increased markedly at later time points (14 and 21 DPI) after i.p. infection of BALB/c mice, whereas in C57BL/6 mice the highest increase was after p.o. infection. Considerable IFN-γ production in ex vivo cultures of Peyer's patch lymphocytes of both mouse strains was observed after p.o. infection. Intraperitoneal infection of SCID mice induced marked influx of inflammatory cells into the peritoneum with NK cells. The results contribute to the development of an animal model of microsporidiosis caused by E. intestinalis.

Słowa kluczowe

EN

Microsporidia; mouse; infection; mice; immunity; immunodeficient mice; immunocompetent mice; immune response; Encephalitozoon intestinalis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2005
• Tom: 50
• Numer: 2
• Opis fizyczny: p.180-188,fig.,ref.

Twórcy

autor

Salat J

• Academy of Sciences of the Czech Republic, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic

autor

Kopecky J.

autor

Janeckova B.

autor

Ditrich O.

Bibliografia

• Achbarou A., Ombrouck C., Gneragbe T., Charlotte F., Rénia L., Desportes-Livage I., Mazier D. 1996. Experimental model for human intestinal microsporidiosis in interferon gamma receptor knockout mice infected by Encephalitozoon intestinalis. Parasite Immunology, 18, 382-392.
• Asmuth D.M., De Girolami P.C., Federman M., Eztratty C.R., Pleskow D.K., Desai G., Wanke C.A. 1994. Clinical features of microsporidiosis in patients with AIDS. Clinical Infectious Diseases, 18, 819-825.
• Bosma M.J., Carroll A.M. 1991. The SCID mouse mutant: definition, characterisation, and potential use. Annual Reviews of Immunology, 9, 323-350.
• Braunfuchsová P., Kopecký J., Ditrich O., Koudela B. 1999. Cytokine response to infection with the microsporidian Encephalitozoon cuniculi. Folia Parasitologica, 46, 91-95.
• Braunfuchsová P., Salát J., Kopecký J. 2001. CD8+ T lymphocytes protect SCID mice against Encephalitozoon cuniculi infection. International Journal for Parasitology, 31, 681-686.
• Braunfuchsová P., Salát J., Kopecký J. 2002. Comparison of the significance of CD4+ and CD8+ T lymphocytes in the protection of mice against Encephalitozoon cuniculi infection. Journal of Parasitology, 88, 797-799.
• Cali A., Kotler D.P., Orenstein J.M. 1993. Septata intestinalis n. g., n. sp., an intestinal microsporidian associated with chronic diarrhea and dissemination in AIDS patients. Journal of Eukaryotic Microbiology, 40, 101-112.
• Canning E.U., Hollister W.S. 1990. Enterocytozoon bieneusi (Microspora): prevalence and pathogenicity in AIDS patients. Transactions of the Royal Society of Tropical Medicine and Hygiene, 84, 181-186.
• Canning E.U., Lom J. 1986. The microsporidia of vertebrates. Academic Press, New York.
• Didier E.S., Rogers L.B., Orenstein J.M., Baker M.D., Vossbrinck C.R., Van Gool T., Hartskeerl R., Soave R., Beaudet L.M. 1996. Characterisation of Encephalitozoon (Septata) intestinalis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. Journal of Eukaryotic Microbiology, 43, 34-43.
• Didier E.S., Varner P.W., Didier P.J., Aldras A.M., Millichamp N.J., Murphey-Corb M., Bohm R., Shadduck J.A. 1994. Experimental microsporidiosis in immunocompetent and immunodeficient mice and monkeys. Folia Parasitologica, 41,1-11.
• El Fakhry Y., Achbarou A., Franetich J., Desportes-Livage I., Mazier D. 2001a. Dissemination of Encephalitozoon intestinalis, a causative agent of human microsporidiosis, in IFN-γ receptor knockout mice. Parasite Immunology, 23, 19-25.
• El Fakhry Y., Achbarou A., Desportes I., Mazier D. 2001b. Resistance to Encephalitozoon intestinalis is associated with interferon-γ and interleukin-2 cytokines in infected mice. Parasite Immunology, 23, 297-303.
• Enriquez F.J., Wagner G., Fargoso M., Ditrich O. 1998. Effect of an anti-exospore monoclonal antibody on microsporidial development in vitro. Parasitology, 117, 515-520.
• Franzen C., Muller A. 2001. Microsporidiosis: human diseases and diagnosis. Microbes and Infection. 3, 389-400.
• Khan I.A., Moretto M. 1999. Role of gamma interferon in cellular immune response against murine Encephalitozoon cuniculi infection. Infection and Immunity, 67, 1887-1893.
• Khan I.A., Schwartzman J.D., Kasper L.H., Moretto M. 1999. CD8+ CTLs are essential for protective immunity against Encephalitozoon cuniculi infection. Journal of Immunology, 162, 6086-6091.
• Koudela B., Vítovec J., Kučerová Z., Ditrich O., Trávníček J. 1993. The severe combined immunodeficient mouse as model for Encephalitozoon cuniculi microsporidiosis. Folia Parasitologica, 40, 279-286.
• Liu J. J., Greeley E.H., Shadduck J. A. 1989. Mechanisms of resistance/susceptibility to murine encephalitozoonosis. Parasite Immunology, 11, 241-256.
• Niederkorn J.Y., Brieland J.K., Mayhew E. 1983. Enhanced natural killer activity in experimental murine encephalitozoonosis. Infection and Immunity, 41, 302-307.
• Niederkorn J.Y., Shadduck J.A., Schmidt E.C. 1981. Susceptibility of selected inbred strains of mice to Encephalitozoon cuniculi. Journal of Infectious Diseases, 144, 246-253.
• Salát J., Braunfuchsová P., Kopecký J. 2001. Experimental infection of immunocompetent and immunodeficient mice with Encephalitozoon cuniculi. Folia Parasitologica, 48, 249-254.
• Salát J., Braunfuchsová P., Kopecký J., Ditrich O. 2002. Role of CD4+ and CD8+ T lymphocytes in the protection of mice against Encephalitozoon intestinalis infection. Parasitology Research, 88, 603-608.
• Salát J., SakB., LeT., Kopecký J. 2004. Susceptibility of IFN-γ or IL-12 knock-out and SCID mice to infection with two microsporidian species, Encephalitozoon cuniculi and E. intestinalis. Folia Parasitologica, 51, 275-282.
• Schmidt E.C., Shadduck J.A. 1983. Murine encephalitozoonosis model for studying the host-parasite relationship of chronic infection. Infection and Immunity, 40, 936-942.
• Schmidt E.C., Shadduck J. A. 1984. Mechanisms of resistance to the intracellular protozoan Encephalitozoon cuniculi in mice. Journal of Immunology, 133, 2712-2719.
• Weber R., Bryan R.T., Owen R.L., Wilcox C.M., Gorelkin L., Visvesvara G.S. 1992. Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates. New England Journal of Medicine, 326, 161-166.