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Czasopismo

1992 | 37 | 4 |

Tytuł artykułu

Investigations on origin of alpha-amylase present in Ascaris suum intestine using immunodiffusion test

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The provenance of α-amylase from intestines of adult Ascaris suum was investigated by Ouchterlony method using sera of guinea pigs vaccinated with the enzyme protein extracted from the worm intestine or pig pancreas. No cross-reactivity was observed between sera raised against pig α-amylase and A. suum enzyme. The results support suggestions that α-amylase present in A. suum intestine is produced by the nematode and not required from the host intestine.

Wydawca

-

Czasopismo

Rocznik

Tom

37

Numer

4

Opis fizyczny

p.205-207,fig.

Twórcy

autor
  • Department of General Biology, Higher Teacher's School, Zolnierska 14, 10-561 Olsztyn, Poland

Bibliografia

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  • Brand T. von 1973. Biochemistry of parasites. Academie Press, New York and London, 106-112.
  • Brown T., Wold F. 1981. Detection of alpha-amylase activity in unprocessed preamylase produced in the cell-free translation of porcine pancreatic RNA. Journal of Biological Chemistry, 256, 10743-10746.
  • Cozzone P., Pasero L., Beaupoil B., Marchis-Mouren G. 1970. Characterization of porcine pancreatic isoamylases. Chemical and physical studies. Biochimica et Biophysica Acta, 207,490-504.
  • Davis B. J. 1964. Disc electrophoresis. Method and application to human serum proteins. Annals of the New York Academy of Sciences, 121, 404-427.
  • Geiger B., Ben-Yoseph Y., Arnon R. 1976. Immunological relationships among hexosaminidases of different species. Immunochemistry, 13, 485-490.
  • Gerber M., Naujiks K., Lenz H., Wulff K. 1987. A monoclonal antibody that specifically inhibits human salivary α-amylase. Clinical Chemistry, 31, 14—19.
  • Kamihira M., Taniguchi M., Iijima S., Kobayashi T. 1988. Purification of recombinant α-amylase with immuno-affinity chromatography using monoclonal antibody. Journal of Fermentation Technology, 66, 625-631.
  • Karpiak S. E., Sobiech K. A. 1981. Natural low molecular weight organic compounds as alpha-amylase differentiating factors. Bulletin de l'Academie Polonaise des Sciences, Série des Sciences Biologiques, 29, 195-199.
  • Martzen M. R., Geise G. L., Peanasky R. J. 1986. Ascaris suum: Immunoperoxidase and fluorescent probe analysis of host proteases and parasite inhibitors in developing eggs and second stage larvae. Experimental Parasitology, 61, 138-145.
  • McGeachin R. L., Reynolds J. M. 1959. Differences in mammalian amylases demonstrated by enzyme inhibition with specific antisera. Journal of Biological Chemistry, 234, 1456-1458.
  • Messer H., Dean R. T. 1975. Immunochemical relationship between α-amylases of rat liver, serum, pancreatic paratid gland. Biochemical Journal, 151,17-22.
  • Mifflin T. E., Benjamin C. D., Buns D. E. 1985. Rapid quantitative, specific measurement of pancreatic amylase in serum with use of monoclonal antibody. Clinical Chemistry, 31,1283-1288.
  • Ouchterlony O. 1958. Diffusion in gel methods for immunological analysis. Progress in Allergy, 5,1-78.
  • Prusak E., Kurowska E., Szewczuk A. 1983. Immunological studies of γ-glutamyltransferases from various animal organs using a sensitive electrophoretic method. Archivum Immunologiae et Therapiae Experimentalis, 31, 827-832.
  • Rauscher E., Gerber M. 1989. Pancreatic α-amylase assay employing the synergism of two monoclonal antibodies. Clinica Chimica Acta, 183,41-44.
  • Rogers W. P. 1940. Digestion in parasite nematodes. I. The digestion of carbohydrates. Journal of Helminthology, 18, 143-154.
  • Sobiech K. A., Słowińska R. 1981. Purification of anti-amylase antibodies by affinity chromatography and their reaction with alpha-amylase. Bulletin del’ Academie Polonaise des Sciences, Série des Sciences Biologiques, 34, 209-213.
  • Sobiech K. A., Słowińska R. 1983. Enzyme immunoassay for alpha- amylase. Journal of Immunoassay, 4,127-134.
  • Sobiech K. A., Słowińska R., Osada J., Simoni G. 1983. Determination of amylase by measurement of enzymatic activity and by enzyme immunoassay and radioimmunoassay. Archivum Immunologiae et Therapiae Experimentalis, 31, 845.
  • Strumeyer D. H., Kao W., Roberts T., Forsyth-Davis D. 1988. Isozymes of α-amylase in the porcine pancreas: population distribution. Comparative Biochemistry and Physiology, 91B, 2, 351-357.
  • Żółtowska K. 1988. Oczyszczanie i niektóre własności alfa-amylazy z Ascaris suum (Nematoda). Materiały XXIV Zjazdu Polskiego Towarzystwa Biochemicznego, 19-21, IX, 1988, Poznań, 131.
  • Żółtowska K. 1991. Distribution of enzymes hydrolizing carbohydrates in the alimentary tract of Ascaris suum. Acta Parasitologica Polonica, 36, 93-96.

Typ dokumentu

Bibliografia

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Identyfikator YADDA

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