Vitellogenesis in virgin and mated females of the mealworm beetle, Tenebrio molitor

• Autorzy: Michalik J. , Chojnicka B. , Cymborowski B.
• Języki publikacji: EN

Abstrakty

EN

A comparison between vitellogenesis in virgin and mated females of Tenebrio molitor showed significant differences at each investigated developmental stage. Yolk protein deposition in oocytes, measured as an increase in their size parameters (length, width, and volume), proceeded much faster and was more efficient in mated females as compared to virgins. In fertilized females the gonadotropic cycle showed a cyclicity with an eight-day period while virgin females finish their vitellogenic stage after the first cycle. These differences were reflected in changes in the rate of protein synthesis in the fat body of females completing vitellogenesis or entering the next oogenetic cycle. In the haemolymph, in addition to a large (158 kDa) and two small (56 kDa and 45 kDa) subunits of vitellogenin, there was an abundance of proteins of 80 kDa and 60 kDa.

Słowa kluczowe

EN

yolk; oogenetic cycle; protein; virgin female; Tenebrio molitor; hemolymph; oocyte; mealworm beetle; vitellogenesis; protein synthesis

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1996
• Tom: 43
• Numer: 4
• Opis fizyczny: p.623-631,fig.

Twórcy

autor

Michalik J.

• Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Chojnicka B.

autor

Cymborowski B.

Bibliografia

• 1. Engelmann, F. (1979) Insect vitellogenins: Iden­tification, biosynthesis and role of vitello­genesis. Adv. Insect Physiol 14,49-108.
• 2. Kunkel, J.G. & Nordin, J.H. (1985) Yolk proteins; in Comprehensive Insect Physiology Biochemistry and Pharmacology (Kerkut, G.A. & Gilbert, LI., eds.) vol. 1, pp. 83-110, Pergamon Press, Oxford.
• 3. Harnish, D.G., Wyatt, G.R. & White, B.N. (1982) Insect vitellins: Identification of primary pro­ducts of translation. J. Exp. Zool. 220,11-19.
• 4. Harnish, D.G. & White, B.N. (1982) Insect vi­tellins: Identification, purification, and charac­terization from eight orders. J. Exp. Zool. 220, 1-10.
• 5. Engelmann, F. (1984) The regulation of vitello­genesis in insects: The pleiotropic role of juve­nile hormones; in Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones (Hoff­mann, J.A. & Porchet, M., eds.) pp. 444 153, Springer-Verlag, Berlin.
• 6. Web, T.J. & Hurd, H. (1995) Microsomal juvenile hormone binding proteins in the follicle cells of Tenebrio molitor. Insect Biochem. Molec. Biol. 25, 631-637.
• 7. Brad field, J.Y., Berlin, R.L. & Keeley, LL. (1990) Contrasting modulations of gene expression by juvenile hormone analog. Insect Biochem. 20, 105-111.
• 8. Davey, K.G., Sevala, V.L. & Gordon, D.K.B. (1993) The action of juvenile hormone and anti- gonadotropin on the follicle cells of Locusta migratoria. Invert. Reprod. Dei>. 24, 39-46.
• 9. Hagedorn, H.H. & Kunkel, J.G. (1979) Vitello­genin and vitellin in insects. Ann. Rev. Entomol. 24,473-505.
• 10. Hagedorn, H.H. (1985) The role of ecdysteroids in reproduction; in Comprehensive Insect Bio­chemistry and Pharmacology (Kerkut, G.A. & Gilbert, L.I., eds.) vol. 8, pp. 205-261, Pergamon Press, Oxford.
• 11. Carliste, J. & Loughton, B.G. (1986) The inhi­bition of protein synthesis in Locusta migratoria bv adipokinetíc hormone. /. Insect Physiol. 32, 573-578.
• 12. Carliste, J. & Loughton, B.G. (1984) A protein synthesis stimulating hormone in the locust; in Insect Neurochemistry and Neurophysiology (Borkovec, A.B. & Kelly, T.J., eds.) pp. 341-344, Plenum, New York.
• 13. Mordue, W. (1965) The neuro-endocrine control of oocyte development in Tenebrio molitor L. /. Insect Physiol. 11, 505-511.
• 14. [¿verdure, A.-M. (1972) L'evolution de 1'ovaire chez la femelle adulte de Tenebrio molitor — la vitellogenese. J. Insect Physiol. 18,1369-1385.
• 15. Gerber, G.H. (1975) Reproductive behaviour and physiology of Tenebrio molitor (Coleóptera: Tenebrionidae). II. Egg development and opposition in young females and the effects of mating. Can. Entomol. 107,551-559.
• 16. Rosiński, G., Wrzeszcz, A. & Obuchowicz, L. (1979) Differences in trchalase activity in the intestine of fed and starved larvae of Tenebrio molitor L. Insect Biochem. 9,485-488.
• 17. Ullmann, S.L. (1973) Oogenesis in Tenebrio molitor: histological and autoradiographical observations on pupal and adult ovaries. /. Embryol. Exp. Morphol. 30,179-217.
• 18. Mans, R.J. & Novelli, G.D. (1961) Measurement of the incorporation of radioactive amino acids into protein by filter-paper disc method. Arch. Biochem. Biophys. 94,48-53.
• 19. Linzen, B. & Wyatt, G.R. (1964) The nucleic acid content of tissues of Cecropia silkmoth pupae. Biochim. Biophys. Acta 87,18&-197.
• 20. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72,248-154.
• 21. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227,680-685.
• 22. Telfer, W.H., Rubenstein, E. & Pan, M. (1981) How the ovary makes yolk in Hyalophora? in Regulation of Insect Development and Behaviour. (Sehnal, F., Zafrfca, A., Menn, J.J. & Cymborow- ski, B., eds.) pp. 637-654, Technical University of Wroclaw Press, Poland.
• 23. Hurd, H., Strambi, C. & Beckage, N.E. (1990) Hymenolepis diminuta: An investigation of juve­nile hormone titre, degradation and supple­mentation in the intermediate host, Tenebrio molitor. Parasitology, 100,445-452.
• 24. Ryan, R.O. (1990) Dynamics of insect lipophorin metabolism. ]. Lipid Res. 31,1725-1739.
• 25. Blacklock, B.J. & Ryan, R.O. (1994) Haemo- lymph lipid transport. Insect Biochem. Molec. Biol. 24,855-873.
• 26. Ryan, R.O., Senthilathipan, K.R., Wells, M.A. & Law, J.H. (1988) Facilitated diacylglycerol ex­change between insect hemolymph lipo- phorins. Properties of Manduca sexta lipid transfer particle. /. Biol. Chem. 263,14140-14145.
• 27. Takeuchi, N. & Chino, II. (1993) Lipid transfer particle in the haemolymph of the American cockroach: Evidence for its capacity to transfer hydrocarbons between lipophorin particles. /. Lipid Res. 34,543-551.
• 28. Hirayama, Y. & Chino, H. (1990) lipid transfer particle in locust haemolymph: Purification and characterization. /. Lipid Res. 31,793-799.