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Tytuł artykułu

Effect of vitamin D3 on chymotrypsin activity in the droppings of laying hens after an exposure to cadmium

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EN

Abstrakty

EN
Effect of both vitamin D₃ and cadmium (Cd) on chymotrypsin activity (ACHT) of laying hen has been studied. Ten month-old laying hens were fed a compound feed of HYD-10 ad libitum. The birds were divided into 8 groups (each group consisting of 6 birds) as follows: experimental groups G1, G3, and G5 were supplemented with Cd (0.3; 0.6; and 6.0 mg.kg⁻¹ CdCl₂, respectively) groups G2, G4, and G6 were supplemented with vitamin D₃ (100 I.U./day) at the same Cd concentrations. C₁ was control group without any supplements and C₂ was control group supplemented only with vitamin D₃. The study lasted 6 months. In groups G1-G4, ACHT activities in the end of the study were rather high (2097.4; 2984; 1805.4, and 2575.9 U.g⁻¹,respectively). The ACHT activity in groups G2, G4 and G6 was by little higher than that in groups G1, G3, and G5 without vitamin D₃ supplementation. The principle of the action of vitamin D₃ via Ca ions and calmodulin is further discussed.

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-

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Tom

44

Numer

2

Opis fizyczny

p.227-233,fig.

Twórcy

autor
  • University Veterinary Medicine, 041 81 Kosice, Slovak Republic
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Bibliografia

  • 1. Akiyama K., Sutoo D., R. Eid D.G.: A 1H - NMR comparison of calmodulin activation by Ca and by Cd. Japanese Journal pharmacology, 1990, 9, 393 - 401.
  • 2. Bauman V., Valinietse M., Babarykin D.: Interactions of vitamin D₃, Ca and heavy metals in chicks. Proceedings of the Latvian Academy of Sciences, Lection B, Natural Sciences, 1994, 1, 70-72.
  • 3. Blahovec J., Blahovcová K., Bartik M.: Arylamidazova aktivita v niektorých orgánoch kurčiat. Veterinárna medicina, 1971, 16, 319- 327.
  • 4. Bokori J., Fekete S., Glavitis R., Kadar I., Koncz J., Kovarij L.: Complex study of the physiological role of cadmium. IV. Effect of prolonged dietary exposure of broiler chickens to cadmium. Acta Veterinaria Hungarica, 1996, 44, 57-94.
  • 5. Dobrzansky Z., Bodák E., Garecka H., Wisniewski J.: Wpływ eksperymentalnej intoksykacji kur niesnych metalami ciezkimi na stopien ich kumulacji w tkankach, narzadach i jajach kurznych. X. kongres Polskiego towarzystwa nauk weter. Wroclaw, 1996, 2, 279-280.
  • 6. Dunn M. A., Too S.L., Ushizaki A.S.: Characterization of a novel protein in chick intestine that exhibits calcium binding activity and regulation by dietary Ca, Al and vitarnin D₃. Journal of Nutrition, 1995, 125, 2916-2924.
  • 7. El - Sebai A., Abaza M., Szilagyi M., Szalay I., Sankari M., Pais I., Anke M., Meissner D.: Physiological and biochemical parameters in chicken exposed to cadmium Mengen and Spurenelementen. 14. Arbeitsstagung, Jena, 1994, 14, 435-440.
  • 8. Foulkes E.C.: On the machanism of cellular Cd uptake. Biological of Trace Elements Research, 1989, 21, 120-195.
  • 9. Friberg L.: Cadmium and the kidney. Environ. Health Perspect., 1984, 52, 1-11.
  • 10. Fullmer C.S.: Dietary calcium levels and treatment interval determine the effect of lead ingestion on plasma 1, 25 - dihydroxyvitamin D concentration in chick. Journal of Nutrition, 1995, 125, 1328-1333.
  • 11. Khandelwal S., Agnihotri N., Tandon S.K.: Biochemical response to cadmium. Dose - time effect. Biological of Trace Elements Research: 1991, 29, 157-164.
  • 12. Kottferová, J., Koréneková, B.: Mechanizmus účinku kadmia na živý organizmus, Veterinářství, 1996, 12, 533-534.
  • 13. Müller, S., Stacey, N.H.: Subclinical response to cadmium in liver ceels. Biological of Trace Elements Research, 1989, 21, 317-323.
  • 14. Neměčková, A., Musil, J.: Lékarská chemie a biochemie. Avicenum Praha Osveta, 1990, 82-83.
  • 15. Rosival, I., Šulík E., Šlesárová L., Korének M., Blahovec J.: Determination of chymotrypsin activity in animal stooIs. Folia veterinaria, 1993, 37, 19-22.
  • 16. Rothe S., Kollmer W.E. Rambeck W.A.: Influence of certains factors on cadmium retention. Revue de Medicine Veterinarie, 1992, 3, 255-260.
  • 17. Siklenka P., Jacková A., Hurná E., Koréneková B., Kottferová J., Kondrát V., Šály J.: Záverečná správa 165-05, Biotechnické postupy v ochrane a tvorbe zdravia a kvalite produktov zvierat, UEVM Košice 1994.
  • 18. Sugawara C., Sugawara N.: The effect of cadmium on soluble proteins, enzymes and essential metals of the duodenal mucosa Archive of Environmental Contamination Toxicology, 1997, 6, 121-128.
  • 19. Suzuki Y.: J. Camium metabolism and toxicity in rats after longterm subcutaneous administration. Toxicology of Environmental Health, 1980, 6, 469-482.
  • 20. Šulík E., Korének M., Šlesárová L., Blahovec J., Rosival I.: Photometric determination of chymotrypsin activity in feaces Materiały Kongresu, Olsztyn, 1992, 9, 496.
  • 21. Tsuritany I., Honda R., Ishizaki, M., Jamada Y., Kido T., Nogawa T.: Impairment of vitamin D metabolism due to enviromental cadmium exposure and possible relevance to sex - related differences in value ability to the bonedamage. Journal of Toxicology and Enviromental Health, 1992, 37, 519-533.
  • 22. Winkler C., Rambeck W.A.: Der Einfluss der Calcium-, Phosphor -, und Vitamin D - Zufuhr auf die Cadmium Retention in Leber und Nieren beim wachsenden Huhn Tiernährung und Futtermittel, 1984, 51, 250-256.

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Bibliografia

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