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2005 | 54 | 3 |

Tytuł artykułu

Cloning and preliminary characterization of a GATC-specific beta2-class DNA:m6A methyltransferase encoded by transposon Tn1549 from Enterococcus spp.

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A recent study revealed a subfamily of N6-adenine (m⁶A) methyltransferases that comprises a few functionally studied eukaryotic members acting on mRNA and prokaryotic members acting on DNA as well as numerous uncharacterized open reading frames. Here, we report cloning and functional characterization of a prokaryotic member of this family encoded by transposon Tn1549 from Enterococcus spp.

Wydawca

-

Rocznik

Tom

54

Numer

3

Opis fizyczny

p.249-252,fig.,ref.

Twórcy

autor
  • University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
autor

Bibliografia

  • Bujnicki J.M. 2002. Sequence permutations in the molecular evolution of DNA methyltransferases. BMC Evol. Biol. 2: 3.
  • Bujnicki J.M., M. Feder, M. Radlińska and R.M. Blumenthal. 2002. Structure prediction and phylogenetic analysis of a functionally diverse family of proteins homologous to the MT-A70 subunit of the human mRNA: m⁶A methyltransferase. J. Mol. Evol. 55: 431-44.
  • Bujnicki J.M., M. Radlińska, R Zaleski and A. Piekarowicz. 2001. Cloning of the Haemophilus influenzae Dam methyltransferase and analysis of its relationship to the Dam methyltransferase encoded by the HP1 phage. Acta. Biochim. Pol. 48: 969-83.
  • Chen L., D.B. Paulsen, D.W. Scruggs, M.M. Banes, B.Y. Reeks and M.L. Lawrence. 2003. Alteration of DNA adenine methylase (Dam) activity in Pasteurella multocida causes increased spontaneous mutation frequency and attenuation in mice. Microbiology 149: 2283-90.
  • Dryden D.T. 1999. Bacterial DNA methyltransferases. p. 283-340. In: Cheng X, Blumenthal RM (eds) S-Adenosylmethionine-dependent methyltransferases: structures and functions. World Scientific Publishing, NJ.
  • Garnier F., S. Taourit, P. Glaser, P. Courvalin and M. Galimand. 2000. Characterization of transposon Tn/549, conferring VanB-type resistance in Enterococcus spp. Microbiology 146 1481-9.
  • Heithoff D.M., R.L. Sinsheimer, D.A. Low and M.J. Mahan. 1999. An essential role for DNA adenine methylation in bacterial virulence. Science 284: 967-70.
  • Herman G.E. and P. Modrich. 1982. Escherichia coli dam methylase. Physical and catalytic properties of the homogeneous enzyme. J. Biol. Chem. 257: 2605-2612.
  • Hermann A. and A. Jeltsch. 2003. Methylation sensitivity of restriction enzymes interacting with GATC sites. Biotechniques 34: 924-6, 928, 930.
  • Malone T., R.M. Blumenthal and X. Cheng. 1995. Structure-guided analysis reveals nine sequence motifs conserved among DNA amino-methyltransferases, and suggests a catalytic mechanism for these enzymes. J. Mol. Biol. 253: 618-632.
  • Matveyev A.V., K.T. Young, A. Meng and J. Elhai. 2001. DNA methyltransferases of the cyanobacterium Anabaena PCC 7120. Nucleic. Acids. Res. 29: 1491-506.
  • McClelland M., M. Nelson and E. Raschke. 1994. Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucleic. Acids. Res. 22: 3640-3659.
  • Noyer-Weidner M. and T.A. Trautner. 1993. Methylation of DNA in prokaryotes. EXS 64: 39-108.
  • Piekarowicz A. and J.M. Bujnicki. 1999. Cloning of the Dam methyltransferase gene from Haemophilus influenzae bacteriophage HP I. Acta Microbiol. Pol. 48: 123-9.
  • Radlińska M. and J.M. Bujnicki. 2001. Cloning of enterohemorrhagic Escherichia coli phage VT-2 dam methyltransferase. Acta Microbiol. Pol. 50: 161-7.
  • Roberts R.J., T. Vincze, J. Posfai and D. Macelis. 2005. REBASE-restriction enzymes and DNA methyltransferases. Nucleic Acids Res. 33 Database Issue: D230-2.
  • Sambrook J., D.W. Russell and J. Sambrook. 2002. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
  • Scarano M.L, M. Strazzullo, M.R. Matarazzo and M. D'Esposito. 2005. DNA methylation 40 years later: Its role in human health and disease. J. Cell. Physiol.
  • Siksnys V., N. Zareckaja, R. Vaisvila, A. Timinskas, P. Stakenas, V. Butkus and A. Janulaitis. 1994. CAATTG-specific restriction-modification muni genes from Mycoplasma: sequence similarities between R.MunI and R.EcoRI. Gene 142: 1-8.
  • Watson M.E., Jr., J. Jarisch and A.L. Smith. 2004. Inactivation of deoxyadenosine methyltransferase (dam) attenuates Haemophilus influenzae virulence. Mol. Microbiol. 53: 651-64.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-025fa0a9-8741-4471-8a17-c1dbd628ad2f
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