Modulation of human deoxycytidine kinase activity as a response to cellular stress induced by NaF

• Autorzy: Csapo Z. , Sasvari-Szekely M. , Spasokoukotskaja T. , Staub M.
• Języki publikacji: EN

Abstrakty

EN

Deoxycytidine kinase (dCK) is one of the key enzymes of deoxynucleoside salvage sup­plying resting lymphocytes with DNA precursors for synthesis and repair. The level of dCK activity is especially important in chemotherapy with the use of deoxynucleoside ana­logues like arabinosyl cytosine (Citarabid, ara-C), or 2-chloro-deoxyadenosine (Cladribine, CdA). Previous results showed that Cladribine treatment of human lympho­cytes increased several fold the activity of dCK without increasing the amount of dCK pro­tein itself (Sasvari-Székely, et al., 1998, Biochem. Pharmacol. 56, 1175), and a possible post-translational modification was suggested. This theory was further investigated using NaF as an inhibitor of protein phosphatases. It was shown that NaF treatment of cells ele­vated dCK activity while inhibiting DNA synthesis. The possible mechanism of dCK activa- tion/inactivation induced by exposure of cell cultures to different agents is discussed.

Słowa kluczowe

EN

DNA precursor; arabinosyl cytosine; lymphocyte; 2-chloro-deoxyadenosine; deoxycytidine kinase; tonsil

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 1
• Opis fizyczny: p.251-256,fig.

Twórcy

autor

Csapo Z.

• Semmelweis University, H-1444 Budapest-8, POB 260, Hungary

autor

Sasvari-Szekely M.

autor

Spasokoukotskaja T.

autor

Staub M.

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