Phosphomannose isomerase gene for selection in lettuce [Lactuca sativa L.] transformation

• Autorzy: Briza J. , Ruzickova N. , Niedermeierova H. , Dusbabkova J. , Vlasak J.
• Języki publikacji: EN

Abstrakty

EN

 A positive selection system using phosphomannose isomerase was employed for Agrobacterium tumefaciens mediated transformation of lettuce (Lactuca sativa L. var. 'Achát'). It was shown that the mannose-based selection system works very well with the lettuce genotype used, reaching up to 25 % transformation efficiency on the medium with 20 g/L mannose and 20 g/L sucrose. The best transformation efficacy with the commonly-used kanamycin at 100 mg/L as a selection agent was 21 %. Southern blot analyses of thirteen chosen mannose-resistant regenerants revealed that some of them have clonal origin, about one-half harbour a single T-DNA copy and one plant contains an incomplete T-DNA segment with only the left part of T-DNA with the pmi gene present in the genomic DNA. The following Northern analysis showed transcriptional activity of the introduced pmi gene in all plants analysed with very high differences in the level of pmi specific mRNA. The results demonstrate that both mannose and kanamycin provide comparable transformation efficiencies in our lettuce genotype. An alternative selection method with mannose as a selection agent is now; available for lettuce transgenosis.

Słowa kluczowe

EN

lettuce; Lactuca sativa; transformation efficiency; Agrobacterium tumefaciens; phosphomannose isomerase gene; selection; genotype; sucrose; mannose; kanamycin

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 1
• Opis fizyczny: p.63-68,fig.,ref.

Twórcy

autor

Briza J.

• Biology Centre of the Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic

autor

Ruzickova N.

autor

Niedermeierova H.

autor

Dusbabkova J.

autor

Vlasak J.

Bibliografia

• An G, Watson BD, Chiang CC (1986) Transformation of tobacco, tomato, potato, and Arabidopsis thaliana using a binary Ti vector system. Plant Physiol 81: 301-305. 
• Aswath CR, Mo SY, Kim DH, Park SW (2006) Agrobacterium and biolistic transformation of onion using non-antibiotic selection marker phosphomannose isomerase. Plant Cell Rep 25: 92-99. 
• Ballester A, Cervera M, Peña L (2008) Evaluation of selection strategies alternative to nptII in genetic transformation of citrus. Plant Cell Rep 27: 1005-1015. 
• Boscariol RL, Almeida WAB, Derbyshire MTVC, Mourao FAA, Mendes BMJ (2003) The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck). Plant Cell Rep 22: 122-128. 
• Bříza J, Pavingerová D, Přikrylová P, Gazdová J, Vlasák J, Niedermeierová H (2008) Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato. Biol Plant 52: 453-461.
• Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81: 1991-1995. 
• Curtis IS, Power JB, Blackhall NW, de Laat AMM, Davey MR (1994) Genotype-independent transformation of lettuce using Agrobacterium tumefaciens. J Exp Bot 45: 1441-1449.
• Degenhardt J, Poppe A, Rösner L, Szankowski I (2007) Alternative selection systems in apple transformation. Acta Hort 738: 287-292.
• Dias BBA, Cunha WG, Morais LS, Vianna GR, Rech EL, de Capdeville G, Aragão FJL (2006) Expression of an oxalate decarboxylase gene from Flammulina sp. in transgenic lettuce (Lactuca sativa) plants and resistance to Sclerotinia sclerotiorum. Plant Pathol 55: 187-193.
• Dubois V, Botton E, Meyer C, Rieu A, Bedu M, Maisonneuve B, Mazier M (2005) Systematic silencing of a tobacco nitrate reductase transgene in lettuce (Lactuca sativa L.). J Exp Bot 56: 2379-2388. 
• Ebmeier A, Allison L, Cerutti H, Clemente T (2004) Evaluation of the Escherichia coli threonine deaminase gene as a selectable marker for plant transformation. Planta 218: 751-758. 
• Erikson O, Hertzberg M, Nasholm T (2004) A conditional marker gene allowing both positive and negative selection in plants. Nat Biotechnol 22: 455-458. 
• Erikson O, Hertzberg M, Nasholm T (2005) The dsdA gene from Escherichia coli provides a novel selectable marker for plant transformation. Plant Mol Biol 57: 425-433. 
• Feeney M, Punja ZK (2003) Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.). In Vitro Cell Dev Biol-Plant 39: 578-585.
• Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML, Brand LA, Fink CL, Fry JS, Galluppi GR, Goldberg SB, Hoffmann NL, Woo SC (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci USA 80: 4803-4807. 
• Fu DL, Xiao YM, Muthukrishnan S, Liang GH (2005) In vivo performance of a dual genetic marker, manA-gfp, in transgenic bentgrass. Genome 48: 722-730. 
• Gadaleta A, Giancaspro A, Blechl A, Blanco A (2006) Phosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation. J Cereal Sci 43: 31-37.
• Gao ZS, Xie XJ, Ling Y, Muthukrishnan S, Liang GH (2005) Agrobacterium tumefaciens-mediated sorghum transformation using a mannose selection system. Plant Biotechnol J 3: 591-599. 
• Haldrup A, Petersen SG, Okkels FT (1998) The xylose isomerase gene from Thermoanaerobacterium thermosulfurogenes allows effective selection of transgenic plant cells using d-xylose as the selection agent. Plant Mol Biol 37: 287-296. 
• Hansen G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 4: 226-231. 
• He ZQ, Duan ZZ, Liang W, Chen FJ, Yao W, Liang HW, Yue CY, Sun ZX, Chen F, Dai JW (2006) Mannose selection system used for cucumber transformation. Plant Cell Rep 25: 953-958. 
• Holsters M, De Waele D, Depicker A, Messens E, Van Montagu M, Schell J (1978) Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet 163: 181-187. 
• Hsiao P, Sanjaya Su R-C, Teixeira da Silva JA, Chan M-T (2007) Plant native tryptophan synthase β1 gene is a non-antibiotic selection marker for plant transformation. Planta 225: 897-906. 
• Jain M, Chengalrayan K, Abouzid A, Gallo M (2007) Prospecting the utility of a /MI/mannose selection system for the recovery of transgenic sugarcane (Saccharum spp. hybrid) plants. Plant Cell Rep 26: 581-590. 
• Joersbo M, Okkels FT (1996) A novel principle for selection of transgenic plant cells: Positive selection. Plant Cell Rep 16: 219-221.
• Joersbo M, Donaldson I, Kreiberg J, Petersen SG, Brunstedt J, Okkels FT (1998) Analysis of mannose selection used for transformation of sugar beet. Mol Breed 4: 111-117.
• Joersbo M, Petersen SG, Okkels FT (1999) Parameters interacting with mannose selection employed for the production of transgenic sugar beet. Physiol Plant 105: 109-115.
• Kawazu Y, Fujiyama R, Noguchi Y (2009) Transgenic resistance to Mirafiori lettuce virus in lettuce carrying inverted repeats of the viral coat protein gene. Transgenic Res 18: 113-120.
• Kim T-G, Kim M-Y, Kim B-G, Kang T-J, Kim Y-S, Jang Y-S, Arntzen CJ, Yang M-S (2007) Synthesis and assembly of Escherichia coli heat-labile enterotoxin B subunit in transgenic lettuce (Lactuca sativa). Protein Expr Purif 51: 22-27. 
• Klimyuk VI, Carroll BJ, Thomas CM, Jones JDG (1993) Alkali treatment for rapid preparation of plant tissue for reliable PCR analysis. Plant J 3: 493-494. 
• Ku JJ, Park YW, Park YD (2006) A non-antibiotic selection system uses the phosphomannose-isomerase (PMI) gene for Agrobacterium-mediated transformation of Chinese cabbage. J Plant Biol 49: 115-122.
• Kunze I, Ebneth M, Heim U, Geiger M, Sonnewald U, Herbers K (2001) 2-Deoxyglucose resistance: a novel selection marker for plant transformation. Mol Breed 7: 221-227.
• Lamblin F, Aimé A, Hano C, Roussy I, Domon J-M, Van Droogenbroeck B, Lainé E (2007) The use of the phosphomannose isomerase gene as alternative selectable marker for Agrobacterium-mediated transformation of flax (Linum usitatissimum). Plant Cell Rep 26: 765-772. 
• Lee BT, Matheson NK (1984) Phosphomannoisomerase and phosphoglucoisomerase in seeds of Cassia coluteoides and some other legumes that synthesize galactomannan. Phytochemistry 23: 983-987.
• Lucca P, Ye XD, Potrykus I (2001) Effective selection and regeneration of transgenic rice plants with mannose as selective agent. Mol Breed 7: 43-49.
• Michelmore R, Marsh E, Seely S, Landry B (1987) Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens. Plant Cell Rep 6: 439-442.
• Mikhailov RV, Muratova SA, Dolgov SV (2007) Production of transgenic plum plants from vegetative tissues by means of positive selection. Acta Hort 734: 129-138.
• Miles JS, Guest JR (1984) Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli. Gene 32: 41-48. 
• Min B-W, Cho Y-N, Song M-J, Noh T-K, Kim B-K, Chae W-K, Park Y-S, Choi Y-D, Harn C-H (2007) Successful genetic transformation of Chinese cabbage using phosphomannose isomerase as a selection marker. Plant Cell Rep 26: 337-344. 
• Murashige TF, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473-497.
• Negrotto D, Jolley M, Beer S, Wenck AR, Hansen G (2000) The use of phosphomannose isomerase as a selectable marker to recover transgenic plants (Zea mays L.) via Agrobacterium transformation. Plant Cell Rep 19: 798-780.
• O´Kennedy MM, Burger JT, Botha FC (2004) Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase. Plant Cell Rep 22: 684-690. 
• Pillegi M, Pereira AAM, dos Santos Silva J, Pillegi SAV, Verma DPS (2001) An improved method for transformation of lettuce by Agrobacterium tumefaciens with a gene that confers freezing resistance. Braz Arch Biol Technol 44: 191-196.
• Privalle LS, Wright M, Reed J, Hansen G, Dawson J, Dunder EM, Chang Y-F, Powell ML, Meghji M (2000) Phosphomannose isomerase, a novel selectable plant selection system: mode of action and safety assessment. In Proceedings of the 6th international symposium on the biosafety of genetically modified organisms. Fairbairn C, Scoles G, McHughen A, eds, pp 171-178. University Extension Press, Univ. Saskatchewan, Saskatoon.
• Ramesh SA, Kaiser BN, Franks T, Collins G, Sedgley M (2006) Improved methods in Agrobacterium-mediated transformation of almond using positive (mannose/pmi) or negative (kanamycin resistance) selection-based protocols. Plant Cell Rep 25: 821-828. 
• Reed J, Privalle L, Powell ML, Meghji M, Dawson J, Dunder E, Suttie J, Wenck A, Launis K, Kramer C, Chang YF, Hansen G, Wright M (2001) Phosphomannose isomerase: An efficient selectable marker for plant transformation. In Vitro Cell Dev Biol-Plant 37: 127-132.
• Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
• Seitz C, Bruna S, Li H, Hauser B, Forkmann G (2007) Establishment of a mannose-based selection system for the transformation of Torenia hybrids. Acta Hort 743: 139-142.
• Sigareva M, Spivey R, Willits M, Kramer C, Chang YF (2004) An efficient mannose selection protocol for tomato that has no adverse effect on the ploidy level of transgenic plants. Plant Cell Rep 23: 236-245. 
• Stein JC, Hansen G (1999) Mannose induces an endonuclease responsible for DNA laddering in plant cells. Plant Physiol 121: 71-79. 
• Sundar IK, Sakthivel N (2008) Advances in selectable marker genes for plant transformation. J Plant Physiol 165: 1698-1716. 
• Tai T, Tanksley S (1991) A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue. Plant Mol Biol Rep 8: 297-303.
• Todd R, Tague BW (2001) Phosphomannose isomerase: A versatile selectable marker for Arabidopsis thaliana germ-line transformation. Plant Mol Biol Rep 19: 307-319.
• Torres AC, Cantliffe DJ, Laughner B, Bieniek M, Nagata R, Ashraf M, Ferl RJ (1993) Stable transformation of lettuce cultivar South Bay from cotyledon explants. Plant Cell Tissue Org Cult 34: 279-285.
• Wilmink A, Dons JJM (1993) Selective agents and marker genes for use in transformation of monocotyledonous plants. Plant Mol Biol Rep 11: 165-185.
• Wright M, Dawson J, Dunder E, Suttie J, Reed J, Kramer C, Chang Y, Novitzky R, Wang H, Artim-Moore L (2001) Efficient biolistic transformation of maize (Zea mays L.) and wheat (Triticum aestivum L.) using the phosphomannose isomerase gene, pmi, as the selectable marker. Plant Cell Rep 20: 429-436.
• Yamada T, Tozawa Y, Hasegawa H, Terakawa T, Ohkawa Y, Wakasa K (2005) Use of a feedback-insensitive α subunit of anthranilate synthase as a selectable marker for transformation of rice and potato. Mol Breed 14: 363-373.
• You SJ, Liau CH, Huang HE, Feng TY, Prasad V, Hsiao HH, Lu JC, Chan MT (2003) Sweet pepper ferredoxin-like protein (pflp) gene as a novel selection marker for orchid transformation. Planta 217: 60-65. 
• Zhang P, Potrykus I, Puonti-Caerlas J (2000) Efficient production of transgenic cassava using negative and positive selection. Transgenic Res 9: 405-415. 
• Zhu YJ, Agbayani R, McCafferty H, Albert HH, Moore PH (2005) Effective selection of transgenic papaya plants with the PMI/Man selection system. Plant Cell Rep 24: 426-432.