Polymorphism in intron 23 of the endothelial nitric oxide synthase gene [NOS3] is not associated with hypertension

• Autorzy: Derebecka N. , Holysz M. , Dankowski R. , Wierzchowiecki M. , Trzeciak W.H.
• Języki publikacji: EN

Abstrakty

EN

Nitric oxide (NO) is synthesised in the vascular endothelium by nitric oxide synthase (NOS3) and is an important factor in the regulation of blood pressure. Impaired syn­thesis of NO due to mutations in the NOS3 gene is associated with hypertension. To date several allelic variants of the NOS3 gene have been identified and their possible linkage with hypertension investigated. We studied the distribution of genotypes and frequency of alleles of the G11T poly­morphism in intron 23 of the NOS3 gene in patients with hypertension and in a control group of healthy individuals. The polymorphism was determined by PCR-RFLP analy­sis. The distribution of genotypes in the patients with hypertension and in the healthy individuals did not differ significantly from the values predicted from Hardy-Weinberg equilibrium for the general population. No major differences in the distribution of the G11T polymorphism in the patients and healthy individuals were found (P > 0.05).

Słowa kluczowe

EN

nitric oxide synthase; polymorphism; nitric oxide; hypertension; cardiovascular system; allele; endothelium; blood pressure; genetic polymorphism

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 1
• Opis fizyczny: p.263-268,fig.

Twórcy

autor

Derebecka N.

• University of Medical Sciences, H.Swiecickiego 6, 60-781 Poznan, Poland

autor

Holysz M.

autor

Dankowski R.

autor

Wierzchowiecki M.

autor

Trzeciak W.H.

Bibliografia

• 1.Ignarro, L.J. (1996) Physiology and pathophysiology of nitric oxide. Kidney /nt.(Suppl.) 55, 2-5.
• 2.Derebecka, N., Holysz, M. & Trzeciak, W.H. (2000) Genes of nitric oxide synthases: Structure, regulation of expression, protein products. Postepy Biochemii 2, 130-139 (in Polish).
• 3.Chen, P.F., Tsai, A.L., Berka, V. & Wu, K.K. (1996) Endothelial nitric oxide synthase. J. Biol. Chem. 271, 14631-14635.
• 4.Marsden, P.A., Heng, H.H.Q., Scherer, S.W., Stewart, R.J., Hall, A.V., Shi, X.M., Tsui, L.C. & Schappert, K.T. (1993) Structure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene. J. Biol. Chem. 268, 17478-17488.
• 5.Huang, P.L., Huang, Z., Mashimo, H., Bloch, K.D., Moskowitz, M.A., Bevan, J.A. & Fishman, M.C. (1995) Hypertension in mice lacking the gene for endothelial nitric oxide synthase. Nature. 377, 239-242.
• 6.Wattanapitayakul, S.K., Mihm, M., Young, A.P. & Bauer, J.A. (2001) Therapeutic implication of human endothelial nitric oxide synthase gene polymorphism. Trends Pharmacol. Sci. 22, 361-368.
• 7.Pulkkinen, A., Viitanen, L., Kareinen, A., Lehto, S., Vauhkonen, I. & Laakso, M. (2000) Intron 4 polymorphism of the endothelial nitric oxide synthase gene is associated with elevated blood pressure in type 2 diabetic patients with coronary heart disease. J. Mol. Med. 78, 372-379.
• 8.Hingorani, A.D., Liang, C.F., Fatibene, J., Lyon, A., Monteith, S., Parsons, A., Haydock, S., Hopper, R.V., Stephens, N.G., O'Shaughnessy, K.M. & Broen, M.J. (1999) A common variant of the endothelial nitric oxide synthase (Glu298-- >Asp) is major risk factor for coronary artery disease in the UK. Circulation 100, 1515-1520.
• 9.Bonnardeaux, A., Nadaud, S., Charru, A., Jeunemaitre, X., Corvol, P. & Soubrier, F. (1995) Lack of evidence for linkage of the endothelial cell nitric oxide synthase gene to essential hypertension. Circulation. 91, 96-102.
• 10.Miyamoto, Y., Yoshihiko, S., Kajiyama, M., Yoshimura, M., Shimasaki, Y., Nakayama, M., Kamitani, S., Harada, M., Ishikawa, M., Kuwahara, K., Ogawa, E., Hamanaka, I., Takahashi, N., Kaneshige, T., Teraoka, H., Akamizu, T., Azuma, N., Yoshimasa, Y., Yoshimasa, T., Itoh, H., Masuda, I., Yasue, H. & Nakao, K. (1998) Endothelial nitric oxide synthase gene is positively associated with essential hypertension. Hypertension. 32, 3-8.
• 11.Nakayama, M., Yasue, H., Yoshimura, M., Shimasaki, Y., Kugiyama, K., Ogawa, H., Motoyama, T., Saito, Y., Ogawa, Y., Miyamota, Y. & Nakao, K. (1999) T-786-->C mutation in the 5'-flanking region of the endothelial nitric oxide synthase gene is associated with coronary spasm. Circulation. 99, 2864-2870.
• 12. World Health Organization-International Society of Hypertension guidelines for the management of hypertension. Guidelines subcommittee. (1999) J. Hypertens 17, 151-183.