Antioxidants and proteolytic enzymes in experimental trichinellosis

• Autorzy: Derda M , Hadas E
• Języki publikacji: EN

Abstrakty

EN

Antioxidant and proteolytic enzymes play an important role during trichinellosis. In the present study we show a statistically significant increase in the enzymatic activity of both enzymatic superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and non-enzymatic total antioxidant status (TAS) occurs in the muscles of mice infected with Trichinella spiralis and T. pseudospiralis. Our research has shown that SOD and catalase activity increase during the 1st week of trichinellosis and remain at an elevated level for at least 10-15 weeks following the infection. The activity of proteolytic enzymes is correlated with intensification of antioxidant activity. This activity disappears gradually and simultaneously with the decrease in the activity of antioxidative enzymes induced by T. spiralis. In the T. pseudospiralis infection, proteolytic activity persist longer together with SOD and catalase activity. Upon comparison of the infection induced by T. spiralis and by T. pseudospiralis it was established that the maximum increase in the activity of all analysed enzymes occurs much earlier in the infection with T. spiralis.

Słowa kluczowe

EN

superoxide dismutase; infection; Trichinella pseudospiralis; protease; mice; glutathione peroxidase; proteolytic enzyme; muscle; mouse; trichinellosis; catalase; antioxidant; Trichinella spiralis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2000
• Tom: 45
• Numer: 4
• Opis fizyczny: p.356-361,fig.

Twórcy

autor

Derda M

• Department of Biology and Medical Parasitology, K.Marcinkowski University of Medical Sciences, 10 Fredry Str., 61-701 Poznan, Poland

autor

Hadas E

• Department of Biology and Medical Parasitology, K.Marcinkowski University of Medical Sciences, 10 Fredry Str., 61-701 Poznan, Poland

Bibliografia

• Aebi H. 1974. Catalase. In: Methods of enzymatic analysis (Ed. H. U. Bergmeyer). Verlag Chemie GmbH, Weinheim, 2, 673-683.
• Beynon R. J., Bond J. S. 1990. Proteolytic enzymes - a practical approach. IRL Oxford University Press.
• Boczoń K., Hadaś E., Wandurska-Nowak E., Derda M. 1996. A stimulation of antioxidants in muscles of Trichinella spiralis infected rats. Acta Parasitologica, 41, 136-138.
• Davies K. J. A. 1993. Protein modification by oxidants and the role of proteolytic enzymes. Free radical damage and antioxidant intervention. Biochemical Society Transaction, 21, 346-353.
• Hadaś E., Derda M., Boczoń K., Wandurska-Nowak E. 1994. Biochemical investigations of the dynamics of proteinase activity at different stages of trichinellosis in mice. Wiadomości Parazytologiczne, 40, 329-333.
• Kehrer J. P. 1993. Free radicals as mediators of tissue injury and disease. Critical Review in Toxicology, 23, 21-48.
• Miller N. J., Rice-Evans C., Davies M. J., Gopinathan V., Milner A. 1993. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science, 84, 407-412.
• Paglia D. E., Valentine W. N. 1967. Studies on the quantitative and qualitative characterisation of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine, 70, 158-169.
• Rivett A. J., Knecht E. 1993. Proteasome location. Current Biology, 3, 127-129.
• Sies H. 1993. Strategies of antioxidant defence. European Journal of Biochemistry, 185, 1261-1273.
• Suttle N. F. 1986. Cooper dificiency in ruminants; recent developments. Veterinary Record, 119, 519-522.
• Woolliams J. A., Wiener G., Anderson P. H., McMurray С. H. 1983. Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of cooper in the blood in various breed crosses of sheep. Research Veterinary Science, 34, 253-256.