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2007 | 10 | 2 |

Tytuł artykułu

Flow cytometry as a method for the evaluation of raw material, product and process in the dairy industry

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Producing dairy products which are safe for consumers requires the constant monitoring of the microbiological quality of raw material, the production process itself and the end product. Traditional methods, still a "gold standard", require a specialized laboratory working on recognized and validated methods. Obtaining results is time- and labor-consuming and do not allow rapid evaluation. Hence, there is a need for a rapid, precise method enabling the real-time monitoring of microbiological quality, and flow cytometry serves this function well. It is based on labeling cells suspended in a solution with fluorescent dyes and pumping them into a measurement zone where they are exposed to a precisely focused laser beam. This paper is aimed at presenting the possibilities of applying flow cytometry in the dairy industry.

Wydawca

-

Rocznik

Tom

10

Numer

2

Opis fizyczny

p.119-121,ref.

Twórcy

  • University of Warmia and Mazury, Oczapowskiego 14, 10-718 Olsztyn, Poland
autor

Bibliografia

  • Clark RG, Pinder AC (1998) Improved detection of bacteria by flow cytometry using a combination of antibody and viability markers. J Appl Mirobiol 84: 577-584.
  • Donnelli CW, Baigent GJ ( 1986) Method for flow cytomet­ric detection of Listeria monocytogenes in milk. Appl En­viron Microbiol 52: 689-695.
  • Gunasekera TS, Attfield PV, Veal DA (2000) A flow cytometry method for rapid detection and enumeration of total bacteria in milk. Appl Environ Microbiol 66: 1228-1232.
  • Gunasekera TS, Veal DA, Attfield PV (2003) Potential for broad applications of flow cytometry and fluorescence techniques in microbiological and somatic cell analyses of milk. Int J Food Microbiol 85: 269- 279.
  • Iannelli D, D’Apice L, Fenizia D, Serpe L, Cottone C, Vis- cardi M, Capparelli R (1998) Simultaneous identification of antibodies to Brucella abortus and Staphylococcus aureus in milk samples by flow cytometry. J Clin Microbi­ol 36: 802-806.
  • Judson P, Van Le L (1997) Flow cytometry. Prim Care Up­date Ob Gyns 4: 87-91.
  • Kantorski J (1996) Podstawy cytometrii przepływowej. Centr Eur J Immunol 21:87-93.
  • Laplace-Buihle C, Hahne K, Hunger W, Tirilly Y, Drocourt JL (1993) Application of flow cytometry to rapid micro­bial analysis in food and drinks industries. Biol Cell 78: 123-128.
  • McClelland RG, Pinder AC (1994) Detection of Salmonella typhimuriumin dairy products with flow cytometry and monoclonal antibodies. Appl Environ Microbol 60: 4255-4262.
  • Yamaguchi N, Sasada M, Yamanaka M, Nasu M (2003) Rapid detection of respiring Escherichia coli 0157:H7 in apple juice, milk, and ground beef by flow cytometry. Cytometry 54: 27-35.

Typ dokumentu

Bibliografia

Identyfikatory

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