Drought-induced changes in the actin cytoskeleton of barley (Hordeum vulgare L.) leaves

• Autorzy: Sniegowska-Swierk K. , Dubas E. , Rapacz M.
• Języki publikacji: EN

Abstrakty

EN

Plants have developed different strategies to adapt to various stress conditions including drought. In the present study the drought-induced changes in the actin filament (AFs) network was studied, for the first time, in two barley cultivars of contrasting drought tolerance level. Detached leaves of drought-tolerant (cv. ‘CAM/B1/ CI’) and drought-susceptible (cv. ‘Maresi’) cultivars were dried under controlled conditions. The water relations as well as the transcript accumulation of actin (ACT11), actin depolymerization factor (ADF1) and dehydrine HVA1) encoding genes were studied using qRT-PCR. Quantitative (the relative fluorescence index; RFI) and qualitative drought-induced changes in AF cytoskeleton were observed following staining with phalloidin. It was noticed that tolerant cultivar was characterized with relative water content decreased during drought treatment which was accompanied by increase in HVA1 expression together with decrease in ACT11 and ADF1 transcripts accumulation induced by drought. In drought-susceptible cultivar the expressions of both ACT11 and ADF1 were slightly lower than those in the control. Drought triggeredan extensive AF cytoskeleton reorganization within different types of leaf-blade cells. Remarkable changes in AF configuration and its increased amount (fluorescence intensity) were observed mainly in drought-tolerant cultivar. In addition, drought-induced changes in AFs were closely associated with chloroplasts. Those AFs probably controlled drought-induced intracellular chloroplast positioning in mesophyll. Based on the results obtained in the present study, the possible role of AF rearrangements in drought response is discussed.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 04
• Opis fizyczny: Article: 73 [13 p.], fig.,ref.

Twórcy

autor

Sniegowska-Swierk K.

• Department of Plant Physiology, University of Agriculture in Krakow, Podluzzna 3, 30-239 Krakow, Poland

autor

Dubas E.

• Franciszek Gorski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland

autor

Rapacz M.

• Department of Plant Physiology, University of Agriculture in Krakow, Podluzzna 3, 30-239 Krakow, Poland

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Identyfikatory

DOI

10.1007/s11738-015-1820-0