Proteomic identification of differentially expressed proteins in mature and germinated maize pollen

• Autorzy: Zhu Y. , Zhao P. , Wu X. , Wang W. , Scali M. , Cresti M.
• Języki publikacji: EN

Abstrakty

EN

The identification of proteins involved in pollen germination and tube growth is important for fundamental studies of fertility and reproduction in flowering plants. We used 2-DE and MALDI-TOF-MS to identify differentially expressed proteins in mature (P0) and 1-h germinated (P1) maize pollen. Among about 470 proteins separated in 2D gels, the abundances of 26 protein spots changed (up- or down-regulation) between P0 and P1. The 13 up-regulated protein spots were mainly involved in tube wall modification, actin cytoskeleton organization, energy metabolism, signaling, protein folding and degradation. In particular, pectin methylesterase, inorganic pyrophosphatase, glucose-1-phosphate uridylyltransferase and rab GDP dissociation inhibitor α are highly up-regulated, suggesting their potential role in pollen tube growth. The down-regulated 13 protein spots mainly include defense-related proteins, pollen allergens and some metabolic enzymes. This study would contribute to the understanding of the changes in protein expression associated with pollen tube development.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 4
• Opis fizyczny: p.1467-1474,fig.,ref.

Twórcy

autor

Zhu Y.

• College of Life Science, Henan Agricultural University, 450002, Zhengzhou, China

autor

Zhao P.

• College of Life Science, Henan Agricultural University, 450002, Zhengzhou, China

autor

Wu X.

• College of Life Science, Henan Agricultural University, 450002, Zhengzhou, China

autor

Wang W.

• Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou, China

autor

Scali M.

• Dipartimento di Scienze Ambientali G. Sarfatti, Universita di Siena, Siena, Italy

autor

Cresti M.

• Dipartimento di Scienze Ambientali G. Sarfatti , Universita di Siena, Siena, Italy

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