The effect of menadione on sarcoplasmic reticulum Ca2plus and contractions of single guinea-pig cardiomyocytes

• Autorzy: Lewartowski B. , Zdanowski K. , Wolska B. M.
• Języki publikacji: EN

Abstrakty

EN

We investigated the effect of 2-methyl-1,4-naphtoquinone (Menadione) on sarcoplasmic reticulum (SR) Ca2²⁺ content and electrically stimulated contractions (ESCs) of single isolated myocytes of guinea-pig ventricular myocardium. The contractures initiated by means of microinjections of caffeine into the close vicinity of the cell were used as an indirect index of the SR Ca²⁺ content. Superfusion of the cells for 45 min with Menadione resulted in gradual disappearance of contractile respones to caffeine, prolongation of time to peak amplitude of ESCs by 48±15% and complete inhibition of postrest and postextrasystolic potentiation. These results are consistent with those of Floreani and Caipenedo (7) who found that Menadione strongly inhibits the SR Ca²⁺ ATPase. Despite depletion of the SR Ca²⁺ the amplitude of ESCs did not change which suggests that contractions were initiated in the cells treated with Menadione by Ca²⁺ derived from the sources other than the SR.

Słowa kluczowe

EN

sarcoplasmic reticulum; ATPase; menadione; cardiomyocyte; excitation-contraction coupling; myocyte; guinea pig; ventricular myocardium

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1991
• Tom: 42
• Numer: 2
• Opis fizyczny: p.221-234,fig.,ref.

Twórcy

autor

Lewartowski B.

• Department of Clinical Physiology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813 Warsaw, Poland

autor

Zdanowski K.

• Department of Clinical Physiology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813 Warsaw, Poland

autor

Wolska B. M.

• Department of Clinical Physiology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813 Warsaw, Poland

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