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2013 | 69 | 08 |

Tytuł artykułu

Peripheral blood lymphocytes B and T and their subpopulations in rabbits infected with rabbit haemorrhagic disease virus (RHDV)

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The paper describes the assessment of B lymphocytes with CD19+ receptor, as well as T lymphocytes with receptor CD5+, and their subpopulations - T lymphocytes with CD4+, CD8+, CD25+ receptors, in rabbits infected with seven haemagglutinating strains of RHDV from various European countries. The aim of the study was to find out if the newly observed strains carry the same or similar immunological properties as the RHDV strains analysed previously. The study was performed on 70 mixed-breed rabbits divided into 7 groups of infected animals (consisting of 5 animals each), corresponding to the analyzed RHDV strains. For each of seven groups of infected animals a corresponding group of control animals was assigned (consisting of 5 animals each). The dynamics of the chosen lymphocytes and their subpopulations was assessed with the use of flow cytometry and monoclonal antibodies. The results of the study enable the authors to conclude that those 7 strains resemble Czech strains and may be qualified as strains with medium immunogenicity, forming one immunotype.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

69

Numer

08

Opis fizyczny

p.484-487,ref.

Twórcy

  • Department of Microbiology and Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland
  • Department of Microbiology and Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland
  • Department of Microbiology and Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland
autor
  • Department of Microbiology and Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland
  • Department of Microbiology and Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland
autor
  • Department of Microbiology and Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland

Bibliografia

  • 1.Deng R. T., Xu Y. F., Yu N. X.: Dynamic observation of RTLA and RABELA cells in the initial stage post immunisation with RHD vaccine (in Chinese). Shanghai J. Anim. Sci. Vet. Med. 1989, 5, 26-28.
  • 2.Deptuła W., Kostrzewa A., Stosik M., Tokarz-Deptu³a B., Wiktorowicz K.: Subpopulations of peripheral blood lymphocytes in rabbits. Nowiny Lek. 1998, 67, 377-382.
  • 3.Hukowska-Szematowicz B., Deptuła W.: Dynamics of peripheral blood lymphocytes in rabbits experimentally infected with two Czech strains of RHD virus. Bull. Vet. Inst. Pulawy 2008, 52, 23-29.
  • 4.Hukowska-Szematowicz B., Deptuła W.: Peripheral blood lymphocytes in rabbits infected with Czech strains, CAMPV-562 and CAMPV-558 of RHD virus. Centr. Europ. J. Immunol. 2008, 33, 8-13.
  • 5.Hukowska-Szematowicz B., Tokarz-Deptuła B., Deptuła W.: Lymphocytes T and their subpopulations in peripheral blood in rabbits experimentally infected with two strains of VHD virus (viral haemorrhagic disease). Pol. J. Environ. Stud. 2005, 14, 550-555.
  • 6.Information and training materials of the Laboratory Animals Section, General Assembly of the Association of Agriculture Engineers and Technicians. p. 26-77, 1987.
  • 7.Marques M. R., Costa-e-Silva A., Águas A. P., Teixeira L., Ferreira P. G.: Early acute depletion of lymphocytes in calicivirus-infected adult rabbits. Vet. Res. Commun. 2010, 34, 659-668.
  • 8.Niedźwiedzka P.: Immunological profile and apoptosis in rabbits experimentally infected with strains of RHDV (rabbit haemorrhagic disease) with different biological features. PhD thesis, University of Szczecin 2008.
  • 9.Niedźwiedzka-Rystwej P., Deptuła W.: Cytometric analysis of lymphocytes T and B in rabbits infected with non-haemaglutinogenic strains of RHD virus (rabbit haemorrhagic disease) - Rainham, Frankfurt and Asturias. Pol. J. Vet. Sci. 2010, 13, 157-162.
  • 10.Niedźwiedzka-Rystwej P., Deptuła W.: Non-specific immunity in rabbits infected with 10 strains of the rabbit haemorrhagic disease virus with different biological properties. Centr. Europ. J. Biol. 2010, 5, 613-632.
  • 11.Regulation of the Minister of Agriculture and Rural Development of 10 March 2006 on detailed conditions for maintenance of laboratory animals in experimental units, breeding units and suppliers (Polish Journal of Laws of 2006, No. 50, item 368).
  • 12.Teixeira L., Marques R. M., Águas A. P., Ferreira P.: Regulatory T cells are decreased in acute RHDV lethal infection of adult rabbits. Vet. Immunol. Immunopathol. 2012, 148, 343-347.
  • 13.Tokarz-Deptuła B.: Immunity phenomena in rabbits infected with the RHD (rabbit haemorrhagic disease) virus. Pol. J. Environ. Stud. 2009, 7, 1-81.
  • 14.Tokarz-Deptuła B., Deptuła W.: Dynamic alterations in peripheral blood lymphocytes in rabbits experimentally infected with VHD (viral haemorrhagic disease) virus - Polish strain Kr-1. Pol. J. Vet. Sci. 2003, 6, 67-69.

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Bibliografia

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