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2015 | 18 | 4 |
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Expression of goose parvovirus whole VP3 protein and its epitopes in Escherichia coli cells

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The aim of this study was the expression of goose parvovirus capsid protein (VP3) and its epitopes in Escherichia coli cells. Expression of the whole VP3 protein provided an insufficient amount of protein. In contrast, the expression of two VP3 epitopes (VP3ep4, VP3ep6) in E. coli, resulted in very high expression levels. This may suggest that smaller parts of the GPV antigenic determinants are more efficiently expressed than the complete VP3 gene.
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  • Department of Poultry Viral Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, Poland
  • Department of Poultry Viral Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, Poland
  • Department of Microbiology, Gdansk University of Technology, Gabriela Narutowicza 11/12, 80-233 Gdansk, Poland
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