PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 69 | 3 |
Tytuł artykułu

Calcium-binding proteins in the hippocampal formation of the guinea pig

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Calcium ions (Ca2 ) are known as a second messenger in neurons. However, the concentration of Ca2 should be maintained at an optimal level in view of their high toxicity. Calcium-binding proteins (CaBPs) bind Ca2 with high affi nity. The amount of the CaBPs is age-related and varies during brain development. The aim of this study was to analyze the distribution of CaBPs, parvalbumin (PV) and calbindin D-28k (CB), in the hippocampal formation (the dentate gyrus – DG and hippocampus proper – HP). The studies were carried out on the brains of newborn (P0) and 80-day-old (P80) guinea pigs. Labelling immunofl uorescence was performed on 10 μm-thick frozen sections. PV- and CB-immunoreactive (PV-IR, CB-IR) structures were identifi ed with antibodies against PV (1:2000, code P3088) and CB (1:2000, code C9848). Both studied parts are composed of three cellular layers. In the dentate gyrus, PV-IR cells (elongated and oval-shaped) were labelled in the hilus. In the middle (granular) layer only PV-IR dots surrounding negative cells were observed, whereas in the third (molecular) layer no immunoreactive structures were seen. In the hippocampus proper PV-IR neurons (triangular, piriform and oval-shaped) were found in each sector (CA1–CA3) of the pyramidal layer, the same as in the oriens and molecular layers. CB-IR neurons (oval-shaped, densely arranged) were observed mainly in the granular and pyramidal layers. The distribution of the studied calcium-binding proteins was similar in P0 and P80.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
69
Numer
3
Opis fizyczny
p.300
Twórcy
  • Department of Comparative Anatomy, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
autor
  • Department of Comparative Anatomy, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
Bibliografia
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-ab000058-e7f0-46cf-8baa-19f5795b5f8e
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.