LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

• Autorzy: Gajda A. , Posyniak A. , Tomczyk G.
• Języki publikacji: EN

Abstrakty

EN

For the purpose of quantitative determination of doxycycline (DC) residues in tissues, a sensitive liquid chromatography - tandem mass spectrometry (LC-MS/MS) method was developed. The method was used to determine DC residues in chicken tissues (breast and thigh muscle, liver and kidney) after oral administration with drinking water to five-weak-old broiler chickens. The DC was administered for five consecutive days at a therapeutic dose of 10 mg/kg b.w. once a day. The tissues were collected after 6 h, 24 h, 7 d, and 8 d. The method was validated and the decision limit was established for muscle - 109.2 µg/kg, for liver - 326.1 µg/kg, and for kidney - 634.0 µg/kg. The detection limit was 2 µg/kg and the limit of quantification was 5 µg/kg. In a short period after ceasing the treatment, the detected concentrations of DC were much higher than the established maximum residue limit values. The highest residue concentrations of DC were observed in the kidney, followed by the liver and muscle. The lowest concentration of DC was determined in tight muscle.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2014
• Tom: 58
• Numer: 4
• Opis fizyczny: p.573-579,fig.,ref.

Twórcy

autor

Gajda A.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Posyniak A.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Tomczyk G.

• Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Anadon A., Martinez-Larranga M.R., Diaz M.J.: Phrmacokinetics of doxycycline in broiler chickens. Avian Pathol 1994, 23, 79-90.
• 2. Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. OJ 2002, L 221, pp. 8-36.
• 3. Commission Regulation EU No 37/2010. Off J Eur Commun 2010, L15, 1-72.
• 4. Cherlet M., Schelkens M., Croubels S., De Backer P.: Quantitative multi-residue analysis of tetracyclines and their 4-epimers in pig tissues by high-performance liquid chromatography combined with positive-ion electrospray ionization mass spectrometry. Anal Chim Acta 2003, 492, 199-213.
• 5. Cinquina A.L., Longo F., Anastasi G., Giannetti L., Cozzania R.: Validation of a high - performance liquid chromatography method for the determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline in bovine milk and muscle. J Chromatogr A 2003, 987, 227-233.
• 6. Crivineanu M., Trifan V., Paraschiv G.H., Nicorescu V.: Studies regarding the depletion of doxycycline residues in pig meat and organs. Lucrari Stinifice Med Vet 2008, 41, 114-119.
• 7. Croubels S., Baert K., De Busser J., De Backer P.: Residue study of doxycycline and 4-epidoxycycline in pigs medicated via drinking water. The Analyst 1998, 123, 2733-2736.
• 8. Croubels S., Vermeersch H., De Backer P., Santos M.D.F., Remon J.P., Van Peteghem C.: Liquid chromatographic separation of doxycycline and 4-epidoxy cycline in a tissue depletion study of doxycycline in turkeys. J Chromatogr B 1998, 708, 145-152.
• 9. Dorrestein G.M., Bruijne J.J.D., Vulto A.: Bioavailability of doxycycline injectable in pigeons. Acta Vet Scand Suppl 1991, 87, 291-292.
• 10. El-Gendi A.Y.I., Atef M., Amer A.M.M., Kamel G.M.: Pharmacokinetics and tissue distribution of doxycycline in broiler chickens pretreated with either: Diclazuril or halofuginone. Food Chem Toxicology 2010, 48, 3209-3214.
• 11. EMEA Summary Report Doxycycline (2), European Medicines Agency Veterinary Medicines and Inspections, Committee for Veterinary Medicinal Products. Doxycycline - Summary Report (2). EMEA/MRL/270/97, 1997.
• 12. Gajda A., Posyniak A., Żmudzki J., Tomczyk G.: Determination of doxycycline in chicken fat by liquid chromatography with UV detection and liquid chromatography - tandem mass spectrometry. J Chromatogr B, 2013, 928, 113-120.
• 13. Jerome del Castilo R.E.: Tetracyclines. In: Antimicrobial Therapy in Veterinary Medicine, edited by S. Giguere, J.F. Prescott, J.D. Baggot, R.D. Walker, P.M. Dowling, New York, 2006, pp. 31-239.
• 14. Jha V.K., Layachandran C., Singh S.D.: Pharmacokinetic data on doxycycline and distribution in different biological fluids in female goats. Vet Res Commun 1989, 13, 11-16.
• 15. Kung K., Wanner M.: Pharmacokinetics of doxycycline in turkeys and comparison between feed and water medication. Archiv Geflugelkunde 1994, 58, 84-88.
• 16. Laczay P., Semjen G., Lehel J., Nagy G.: Pharmacokinetics and bioavailability of doxycycline in fasted and nonfasted broiler chicken. Acta Vet Hung 2001, 49, 31-37.
• 17. Oka H., Ikai Y., Ito Y., Hayakawa J., Harada K., Suzuki M., Odani H., Maeda K.: Improvement of chemical analysis of antibiotics. XXIII. Identification of residual tetracyclines in bovine tissues by electrospray high-performance liquid chromatography - tandem mass spectrometry. J Chromatogr B 1997, 693, 337-344.
• 18. Posyniak A., Żmudzki J., Semeniuk S., Niedzielska J., Ellis R.: Determination of tetracycline residues in animal tissues by liquid chromatography . Biomed Chromatogr 1998, 12, 294-299.
• 19. Reyes-Herrera I., Schneider M.J., Cole K., Farnell M.B., Blore P.J., Donoghue J.: Concentrations of antibiotic residues vary- between different edible muscle tissues in poultry. J Food Protect 2005, 68, 2217-2219.
• 20. Santos M.D.F., Vermeersch H., Remon J.P., Schelknes M., de Backer P.: Pharmacokinetics and bioavailability of doxycycline in turkeys. J Vet Pharmacol Therap 1996, 19, 274-280.
• 21. Schneider M.J., Lehotay S.J.: Rapid fluorescence screening assay for tetracyclines in chicken muscle. J AOAC Int 2004, 87, 587-591.
• 22. Shalaby A.R., Salama N.A., Abou-Raya S.H., Emam W.H., Mehaya F.M.: Validation of HPLC method for determination of tetracycline residues in chicken meat and liver. Food Chem 2011, 124, 1660-1666.