Response of antioxidant activity to excess copper in two cultivars of Brassica campestris ssp. Chinensis Makino

• Autorzy: Li Y. , Song Y. , Shi G. , Wang J. , Hou X.
• Języki publikacji: EN

Abstrakty

EN

Changes in ascorbic acid content and antioxidant enzyme activities were investigated in non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) leaves of ‘Wutacai’ and ‘Erqing’ exposed to excess copper (Cu). Cu treatment reduced the fresh weight of shoot and root by 57% and 46% in ‘Wutacai’, and 60 and 54% in ‘Erqing’, respectively. The accumulation of copper in leaves was higher in ‘Wutacai’ than that in ‘Erqing’. Compared to the control, ascorbic acid (AsA) contents were significantly decreased after copper treatment in both cultivars, while they were higher in ‘Wutacai’ than in ‘Erqing’, which may explain the higher coppertolerance of ‘Wutacai’ with higher copper accumulation. The higher AsA contents of ‘Wutacai’ resulted from their lower activities of degrading enzymes, such as ascorbate oxydase (AAO) and ascorbate peroxidase (APX), as well as the increasing activity of dehydroascorbate reductase (DHAR) after copper treatment compared with ‘Erqing’. Copper stimulated superoxide dismutase (SOD) activity in both cultivars, but for catalase (CAT), there was little difference between both cultivars. Peroxidases (POD) activity was decreased after copper treatment in ‘Erqing’, while in ‘Wutacai’, it was significantly increased at 14 days, and POD activity was higher in ‘Wutacai’ than that in ‘Erqing’ at 21 and 28 days. Therefore, the induced increasing activity of POD in ‘Wutacai’ also played an important role in its copper tolerance.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 1
• Opis fizyczny: p.155-162,fig.,ref.

Twórcy

autor

Li Y.

• Horticultural Department, Nanjing Agricultural University, Nanjing 210095, China
• State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China

autor

Song Y.

• Horticultural Department, Nanjing Agricultural University, Nanjing 210095, China

autor

Shi G.

• Horticultural Department, Nanjing Agricultural University, Nanjing 210095, China

autor

Wang J.

• Horticultural Department, Nanjing Agricultural University, Nanjing 210095, China

autor

Hou X.

• Horticultural Department, Nanjing Agricultural University, Nanjing 210095, China
• State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China

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