Production of intergeneric allotetraploid between autotetraploid non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and autotetraploid radish (Raphanus sativus L.)

• Autorzy: Sun C.-Z. , Li Y. , Zhang S.-N. , Zheng J.-S.
• Języki publikacji: EN

Abstrakty

EN

Intergeneric hybrids between non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino; 2n = 4x = 40) and radish (Raphanus sativus L.; 2n = 4x = 36) were obtained through ovary culture and embryo rescue. Some hybrid embryos (0.11 per ovary) were produced, but only 4 of them germinated. As most hybrid embryos failed to develop into plantlets directly, plants were regenerated by inducing shoots on the cultured cotyledon and inducing roots on the root induction medium. All hybrid plants were morphologically uniform. They resembled the non-heading Chinese cabbage in the long-lived habit, the plant status, the vernalization requirement and the petiole color, while the petiole shape, leaf venation pattern and flowers were more similar to those of radish. Upon examination of the flowers, these were found to have normal pistil, but rudimentary anthers with non-functional pollen grains. The somatic chromosome number of F1 plants was 38. Analysis of SSR banding patterns provided additional confirmation of hybridity.

Słowa kluczowe

EN

intergeneric allotetraploid; autotetraploid; non-heading cabbage; Chinese cabbage; Brassica campestris ssp.chinensis; radish; Raphanus sativus; embryo culture; simple sequence repeat

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2014
• Tom: 83
• Numer: 1
• Opis fizyczny: p.75-79,fig.,ref.

Twórcy

autor

Sun C.-Z.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, Horticultural College, Nanjing Agricultural University, Nanjing, 210095, China

autor

Li Y.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, Horticultural College, Nanjing Agricultural University, Nanjing, 210095, China

autor

Zhang S.-N.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, Horticultural College, Nanjing Agricultural University, Nanjing, 210095, China

autor

Zheng J.-S.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, Horticultural College, Nanjing Agricultural University, Nanjing, 210095, China

Bibliografia

• 1. Grant V. Plant speciation. New York, NY: Columbia University Press; 1981.
• 2. Haufler CH, Soltis DE. Genetic evidence suggests that homosporous ferns with high chromosome numbers are diploid. Proc NatlAcad Sci USA. 1986;83(12):4389–4393. http://dx.doi.org/10.1073/pnas.83.12.4389
• 3. Shaked H, Kashkush K, Ozkan H, Feldman M, Levy AA. Sequence elimination and cytosine methylation are rapid and reproducibleresponses of the genome to wide hybridization and allopolyploidy inwheat. Plant Cell. 2001;13(8):1749–1759. http://dx.doi.org/10.1105/TPC.010083
• 4. Adams KL, Wendel JF. Novel patterns of gene expression in polyploid plants. Trends Genet. 2005;21(10):539–543. http://dx.doi.org/10.1016/j.tig.2005.07.009
• 5. Chen ZJ, Ni Z. Mechanisms of genomic rearrangements and gene expression changes in plant polyploids. BioEssays. 2006;28(3):240–252.http://dx.doi.org/10.1002/bies.20374
• 6. Beaulieu J, Jean M, Belzile F. The allotetraploid Arabidopsis thaliana- Arabidopsis lyrata subsp. petraea as an alternative model system for thestudy of polyploidy in plants. Mol Genet Genomics. 2009;281(4):421– 435. http://dx.doi.org/10.1007/s00438-008-0421-7
• 7. Ramsey J, Schemske DW. Pathways, mechanisms, and rates of polyploid formation in flowering plants. Ann Rev Ecol Syst. 1998;29(1):467–501.http://dx.doi.org/10.1146/annurev.ecolsys.29.1.467
• 8. Thao NTP, Ureshino K, Miyajima I, Ozaki Y, Okubo H. Induction of tetraploids in ornamental Alocasia through colchicine and oryzalintreatments. Plant Cell Tissue Organ Cult. 2003;72(1):19–25. http://dx.doi.org/10.1023/A:1021292928295
• 9. Möllers C, Iqbal MCM, Röbbelen G. Efficient production of doubled haploid Brassica napus plants by colchicine treatment of microspores.Euphytica. 1994;75(1–2):95–104. http://dx.doi.org/10.1007/BF00024536
• 10. Chen ZJ, Wang J, Tian L, Lee HS, Wang JJ, Chen M, et al. The development of an Arabidopsis model system for genome-wide analysis ofpolyploidy effects. Biol J Linn Soc. 2004;82(4):689–700. http://dx.doi.org/10.1111/j.1095-8312.2004.00351.x
• 11. Lelivelt CLC, Lange W, Dolstra O. Intergeneric crosses for the transfer of resistance to the beet cyst nematode from Raphanus sativus to Brassica napus. Euphytica. 1993;68(1–2):111–120. http://dx.doi.org/10.1007/BF00024160
• 12. Kolte SJ, Bordoloi DK, Awasthi RP. The search for resistance to major diseases of rapeseed and mustard in India. In: Proceedings of the 8thinternational rapeseed congress. Saskatoon: Organizing Committeeof the Eighth International Rapeseed Congress; 1991. p. 219–225.
• 13. Thierfelder A, Hackenberg E, Nichterlein K, Friedt W. Development of nematode-resistant rapeseed genotypes via interspecific hybridization. n: Proceedings of the 8th international rapeseed congress. Saskatoon: Organizing Committee of the Eighth International Rapeseed Congress;1991. p. 269–273.
• 14. Peterka H, Budahn H, Schrader O, Ahne R, Schütze W. Transfer of resistance against the beet cyst nematode from radish (Raphanussativus) to rape (Brassica napus) by monosomic chromosome addition.Theor Appl Genet. 2004;109(1):30–41. http://dx.doi.org/10.1007/s00122-004-1611-2
• 15. Long MH, Xing GM, Okubo H, Fujieda K. Cross compatibility between Brassicoraphanus (Brassica oleracea × Raphanus sativus) andcruciferous crops, and rescuing the hybrid embryos through ovaryand embryo culture. J Fac Agric Kyushu Univ. 1992;37(1):29–39.
• 16. Agnihotri A, Shivanni KR, Lakshmikumaran MS. Micropropagation and DNA analysis of wide hybrids of cultivated Brassica. In:Proceedings of the 8th international rapeseed congress. Saskatoon:Organizing Committee of the Eighth International Rapeseed Congress;1991. p. 151.
• 17. Warwick SI, Black LD. Molecular systematics of Brassica and allied genera (subtribe Brassicinae, Brassiceae) – chloroplast genome and cytodeme congruence. Theor Appl Genet. 1991;82(1):81–92. http:// dx.doi.org/10.1007/BF00231281
• 18. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–497.http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
• 19. Takahata Y. Production of intergeneric hybrids between a C3-C4 intermediate species Moricandia arvensis and a C3 species Brassicaoleracea through ovary culture. Euphytica. 1990;46(3):259–264. http://dx.doi.org/10.1007/BF00027226
• 20. Zhang GQ, Tang GX, Song WJ, Zhou WJ. Resynthesizing Brassica napus from interspecific hybridization between Brassica rapa and B. oleracea through ovary culture. Euphytica. 2004;140(3):181–187. http://dx.doi.org/10.1007/s10681-004-3034-1
• 21. Tashiro Y. Cytogenetic studies on the origin of Allium wakegi Araki. Agr Saga Univ. 1984;56:1–63.
• 22. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW. Ribosomal DNA spacer-length polymorphisms in barley: mendelianinheritance, chromosomal location, and population dynamics. ProcNatl Acad Sci USA. 1984;81(24):8014–8018. http://dx.doi.org/10.1073/pnas.81.24.8014
• 23. Kim JS, Chung TY, King GJ, Jin M, Yang TJ, Jin YM, et al. A sequencetagged linkage map of Brassica rapa. Genetics. 2006;174(1):29–39.http://dx.doi.org/10.1534/genetics.106.060152
• 24. Choi SR, Teakle GR, Plaha P, Kim JH, Allender CJ, Beynon E, et al. The reference genetic linkage map for the multinational Brassica rapagenome sequencing project. Theor Appl Genet. 2007;115(6):777–792.http://dx.doi.org/10.1007/s00122-007-0608-z
• 25. Cui XM, Dong YX, Hou XL, Cheng Y, Zhang JY, Jin MF. Development and characterization of microsatellite markers in Brassica rapa ssp. chinensis and transferability among related species. Agr Sci Chin.2008;7(1):19–31. http://dx.doi.org/10.1016/S1671-2927(08)60018-8
• 26. Nanda Kumar PB, Shivanna KR. Intergeneric hybridization between Diplotaxis siettiana and crop brassicas for the production of alloplasmiclines. Theor Appl Genet. 1993;85(6–7):770–776. http://dx.doi.org/10.1007/BF00225018
• 27. Batra V, Prakash S, Shivanna KR. Intergeneric hybridization between Diplotaxis siifolia, a wild species and crop brassicas. Theor Appl Genet.1990;80(4):537–541. http://dx.doi.org/10.1007/BF00226756
• 28. Leitch IJ, Bennett MD. Polyploidy in angiosperms. Trends Plant Sci. 1997;2(12):470–476. http://dx.doi.org/10.1016/S1360-1385(97)01154-0
• 29. Song K, Lu P, Tang K, Osborn TC. Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution.Proc Natl Acad Sci USA. 1995;92(17):7719–7723. http://dx.doi.org/10.1073/pnas.92.17.7719
• 30. Wendel JF, Schnabel A, Seelanan T. Bidirectional interlocus concerted evolution following allopolyploid speciation in cotton (Gossypium).Proc Natl Acad Sci USA. 1995;92(1):280–284. http://dx.doi.org/10.1073/pnas.92.1.280
• 31. Comai L, Tyagi AP, Winter K, Holmes-Davis R, Reynolds SH, Stevens Y, et al. Phenotypic instability and rapid gene silencing in newly formedArabidopsis allotetraploids. Plant Cell. 2000;12(9):1551–1567. http://dx.doi.org/10.1105/tpc.12.9.1551
• 32. Hanson RE, Islam-Faridi MN, Crane CF, Zwick MS, Czeschin DG, Wendel JF, et al. Ty1-copia-retrotransposon behavior in a polyploid cotton. Chromosome Res. 2000;8(1):73–76. http://dx.doi. org/10.1023/A:1009239522541
• 33. Salina EA, Ozkan H, Feldman M. Subtelomeric repeat reorganization in synthesized amphiploids of wheat. In: Proceedings of theinternational conference on biodiversity and dynamics of systems,North Eurasia. Novosibirsk: ICG; 2000. p. 102–105.
• 34. Liu B, Vega JM, Segal G, Abbo S, Rodova M, Feldman M. Rapid genomic changes in newly synthesized amphiploids of Triticum andAegilops. I. Changes in low-copy noncoding DNA sequences. Genome.1998;41(2):272–277. http://dx.doi.org/10.1139/g98-011

Identyfikatory

DOI

10.5586/asbp.2013.037