Elicitors, SA and MJ enhance carotenoids and tocopherol biosynthesis and expression of antioxidant related genes in Moringa oleifera Lam. leaves

• Autorzy: Saini R.K. , Harish Prashanth K.V. , Shetty N.P. , Giridhar P.
• Języki publikacji: EN

Abstrakty

EN

Moringa oleifera Lam. leaves are rich source of carotenoids (provitamin A) and α-tocopherol (vitamin E), and there is a scope for their further enhancement, through elicitor mediation, thereby a great potential for addressing these vitamins deficiency. In the present study, we report the efficacy of foliar administration of biotic elicitors, carboxy-methyl chitosan and chitosan, and signaling molecules, methyl jasmonate (MJ) and salicylic acid (SA) for enhancement of major carotenoids and α-tocopherol. Highest α-tocopherol content of 49.7 mg/100 g FW was recorded upon foliar application of 0.1 mM SA after 24 h of treatment, which represented a 187.5 % increase in comparison to the untreated control. Similarly, a maximum of 52.6 mg/100 g FW lutein, and 21.8 mg/100 g FW β-carotene content were observed in leaves after 24 h of treatment with MJ, which represented a 54.0 and 20.3 % increase in comparison to the untreated control, respectively. Among the major genes of carotenoid biosynthetic pathway, the expression of lycopene β-cyclase (LCY-b) was maximum influenced after treatment with elicitors and signaling molecules, compared to phytoene synthase and phytoene desaturase, suggesting the LCY-β-mediated enhancement in the production of β-carotene in elicitor treated M. oleifera leaves. Enhanced production of atocopherol under respective elicitor treatment was further supported by 2.0–2.7 fold up-regulation of γ-tocopherol methyl transferase, compared to untreated control. This is the first report on elicitor-mediated enhanced production of tocopherol and carotenoids in foliage of economically important food plant.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 10
• Opis fizyczny: p.2695-2704,fig.,ref.

Twórcy

autor

Saini R.K.

• Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

autor

Harish Prashanth K.V.

• Meat and Marine Sciences Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

autor

Shetty N.P.

• Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

autor

Giridhar P.

• Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

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Identyfikatory

DOI

10.1007/s11738-014-1640-7