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2013 | 35 | 07 |
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Root restriction affected anthocyanin composition and up-regulated the transcription of their biosynthetic genes during berry development in ‘Summer Black’ grape

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Root restriction was applied to ‘Summer black’ grape (Vitis vinifera L. 9 Vitis labrusca L.) to investigate its effect on anthocyanin biosynthesis in grape berry during development. Anthocyanin composition and expression patterns of 16 genes in anthocyanin pathway were thus analyzed. The results showed that the anthocyanin levels in berry skin were significantly increased and the anthocyanin profile was enriched. Gene expression pattern revealed that the increased anthocyanins coincide with the up-regulated expression of all 16 genes investigated, including phenylalanine ammonia-lyase, 4-coumarate CoA ligase, chalcone synthase 1, chalcone synthase 2, chalcone synthase 3, chalcone isomerase, flavanone 3-hydroxylase 1, flavanone 3-hydroxylase 2, flavonoid 30-hydroxylase (F30H), flavonoid 30,50-hydroxylase (F3050H), di-hydroflavonol 4-reductase, leucoanthocyanidin dioxygenase, O-methyltransferases (OMT), UDP-glucose:flavonoid 3-O-glucosyltransferase (3GT), UDP-glucose:flavonoid 5-O-glucosyltransferase (5GT) and glutathione S-transferase (GST). The increased total anthocyanins predominantly resulted from the increase of tri-hydroxylated, methoxylated and monoglycosylated rather than di-hydroxylated, non-methoxylated, and di-glycosylated forms, which might be due to the differential regulation of F3050H/F30H, OMT and 3GT, respectively.
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  • Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Guangxi Nanning Xiangsi Grape Agriculture and Technology Limited Company, Nanning 530007, Guangxi, China
  • Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Guangxi Nanning Xiangsi Grape Agriculture and Technology Limited Company, Nanning 530007, Guangxi, China
  • Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530022, China
  • Grape and Wine Research Institute of Guangxi Agriculture Science Academy, Nanning 530007, China
  • Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Antolin MC, Ayari M, Sanchez-Diaz M (2006) Effects of partial rootzone drying on yield, ripening and berry ABA in potted Tempranillo grapevines with split roots. Aust J Grape Wine Res 12(1):13–20
  • Bindon K, Dry P, Loveys B (2008) Influence of partial rootzone drying on the composition and accumulation of anthocyanins in grape berries (Vitis vinifera cv. Cabernet Sauvignon). Aust J Grape Wine Res 14(2):91–103
  • Bogs J, Ebadi A, McDavid D, Robinson SP (2006) Identification of the flavonoid hydroxylases from grapevine and their regulation during fruit development. Plant Physiol 140(1):279–291
  • Boland AM, Jerie PH, Mitchell PD, Goodwin I (2000a) Long-term effects of restricted root volume and regulated deficit irrigation on peach: I. Growth and mineral nutrition. J Am Soc Hortic Sci 125(1):135–142
  • Boland AM, Jerie PH, Mitchell PD, Goodwin I, Connor DJ (2000b) Long-term effects of restricted root volume and regulated deficit irrigation con peach: II. Productivity and water use. J Am Soc Hortic Sci 125(1):143–148
  • Castellarin S, Di Gaspero G (2007) Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines. BMC Plant Biol 7(1):1–10
  • Castellarin SD, Di Gaspero G, Marconi R, Nonis A, Peterlunger E, Paillard S, Adam-Blondon AF, Testolin R (2006) Colour variation in red grapevines (Vitis vinifera L.): genomic organisation, expression of flavonoid 30-hydroxylase, flavonoid 30,50-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyanins in berry skin. BMC Genomics 7:12
  • Castellarin S, Matthews M, Di Gaspero G, Gambetta G (2007a) Water deficits accelerate ripening and induce changes in gene expression regulating flavonoid biosynthesis in grape berries. Planta 227(1):101–112
  • Castellarin SD, Pfeiffer A, Sivilotti P, Degan M, Peterlunger E, Di Gaspero G (2007b) Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit. Plant Cell Environ 30(11):1381–1399
  • Castillo-Munoz N, Fernandez-Gonzalez M, Gomez-Alonso S, Garcia-Romero E, Hermosin-Gutierrez I (2009) Red-color related phenolic composition of Garnacha Tintorera (Vitis vinifera L.) grapes and red wines. J Agric Food Chem 57(17):7883–7891
  • Chorti E, Guidoni S, Ferrandino A, Novello V (2010) Effect of different cluster sunlight exposure levels on ripening and anthocyanin accumulation in Nebbiolo grapes. Am J Enol Vitic 61(1):23–30
  • Christie PJ, Alfenito MR, Walbot V (1994) Impact of low-temperature stress on general phenylpropanoid and anthocyanin pathways: enhancement of transcript abundance and anthocyanin pigmentation in maize seedlings. Planta 194(4):541–549
  • Conn S, Curtin C, Bézier A, Franco C, Zhang W (2008) Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins. J Exp Bot 59(13):3621
  • Conn S, Franco C, Zhang W (2010) Characterization of anthocyanic vacuolar inclusions in Vitis vinifera L. cell suspension cultures. Planta 231(6):1–18
  • Cortell JM, Halbleib M, Gallagher AV, Righetti TL, Kennedy JA (2007) Influence of vine vigor on grape (Vitis vinifera L. cv. Pinot Noir) anthocyanins. 1. Anthocyanin concentration and composition in fruit. J Agric Food Chem 55(16):6575–6584
  • Dixon DP, Cole DJ, Edwards R (1998) Purification, regulation and cloning of a glutathione transferase (GST) from maize resembling the auxin-inducible type-III GSTs. Plant Mol Biol 36(1):75–87
  • Downey MO, Harvey JS, Robinson SP (2004) The effect of bunch shading on berry development and flavonoid accumulation in Shiraz grapes. Aust J Grape Wine Res 10(1):55–73
  • Dry PR, Loveys BR (1998) Factors influencing grapevine vigour and the potential for control with partial rootzone drying. Aust J Grape Wine Res 4(3):140–148
  • Falginella L, Di Gaspero G, Castellarin SD (2012) Expression of flavonoid genes in the red grape berry of ‘Alicante Bouschet’ varies with the histological distribution of anthocyanins and their chemical composition. Planta 236(4):1–15
  • Gueffroy DE, Kepner RE, Webb AD (1971) Acylated anthocyanin pigments in Vitis vinifera grapes: identification of malvidin-3-(6-p-coumaroyl) glucoside. Phytochemistry 10(4):813–819
  • Guidoni S, Allara P, Schubert A (2002) Effect of cluster thinning on berry skin anthocyanin composition of Vitis vinifera cv.Nebbiolo. Am J Enol Vitic 53(3):224–226
  • He F, Mu L, Yan G-L, Liang N-N, Pan Q-H, Wang J, Reeves MJ, Duan C-Q (2010) Biosynthesis of anthocyanins and their regulation in colored grapes. Molecules 15(12):9057–9091
  • Holton TA, Cornish EC (1995) Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7(7):1071–1083
  • Jeong ST, Goto-Yamamoto N, Kobayashi S, Esaka M (2004) Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins. Plant Sci (Amsterdam, Neth) 167(2):247–252
  • Jing P, Bomser JA, Schwartz SJ, He J, Magnuson BA, Giusti MM (2008) Structure–function relationships of anthocyanins from various anthocyanin-rich extracts on the inhibition of colon cancer cell growth. J Agric Food Chem 56(20):9391–9398
  • Kobayashi S (2009) Regulation of anthocyanin biosynthesis in grapes. J Jpn Soc Hortic Sci 78(4):387–393
  • Kobayashi S, Ishimaru M, Ding CK, Yakushiji H, Goto N (2001) Comparison of UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) gene sequences between white grapes (Vitis vinifera) and their sports with red skin. Plant Sci (Amsterdam, Neth) 160(3):543–550
  • Kobayashi S, Ishimaru M, Hiraoka K, Honda C (2002) Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis. Planta 215(6):924–933
  • Liang ZC, Wu BH, Fan PG, Yang CX, Duan W, Zheng XB, Liu CY, Li SH (2008) Anthocyanin composition and content in grape berry skin in Vitis germplasm. Food Chem 111(4):837–844
  • Manach C, Mazur A, Scalbert A (2005) Polyphenols and prevention of cardiovascular diseases. Curr Opin Lipidol 16(1):77–84
  • Mataa M, Tominaga S (1998) Effects of root restriction on tree development in Ponkan mandarin (Citrus reticulata Blanco). J Am Soc Hortic Sci 123(4):651–655
  • McCloskey LP, Yengoyan L (1981) Analysis of anthocyanins in Vitis vinifera wines and red color versus aging by HPLC and spectrophotometry. Am J Enol Vitic 32(4):257–261
  • Mertens-Talcott SU, Rios J, Jilma-Stohlawetz P, Pacheco-Palencia LA, Meibohm B, Talcott ST, Derendorf H (2008) Pharmacokinetics of anthocyanins and antioxidant effects after the consumption of anthocyanin-rich acai juice and pulp (Euterpe oleracea Mart.) in human healthy volunteers. J Agric Food Chem 56(17):7796–7802
  • Mori K, Sugaya S, Gemma H (2005) Decreased anthocyanin biosynthesis in grape berries grown under elevated night temperature condition. Sci Hortic 105(3):319–330
  • Myers S (1992) Root restriction of apple and peach with in-ground fabric containers. Acta Hortic 322:215–219
  • Nadal M, Lampreave M (2004) The effects of irrigation on the water relations of the grapevine, yield, grape and wine composition of tempranillo CV in mediterranean climate. J Int Sci Vigne Vin 38(1):75–80
  • Naor A, Gal Y, Bravdo B (2002) Shoot and cluster thinning influence vegetative growth, fruit yield, and wine quality of ‘Sauvignon blanc’ grapevines. J Am Soc Hortic Sci 127(4):628–634
  • Ojeda H, Andary C, Kraeva E, Carbonneau A, Deloire A (2002) Influence of pre- and postveraison water deficit on synthesis and concentration of skin phenolic compounds during berry growth of Vitis vinifera cv. Shiraz. Am J Enol Vitic 53(4):261–267
  • Ozden M, Vardin H, Simsek M, Karaaslan M (2010) Effects of rootstocks and irrigation levels on grape quality of Vitis vinifera L. cv. Shiraz. Afr J Biotechnol 9(25):3801–3807
  • Pomar F, Novo M, Masa A (2005) Varietal differences among the anthocyanin profiles of 50 red table grape cultivars studied by high performance liquid chromatography. J Chromatogr A 1094(1–2):34–41
  • Prior RL (2003) Fruits and vegetables in the prevention of cellular oxidative damage. Am J Clin Nutr 78(3):570–578
  • Sarma AD, Sharma R (1999) Anthocyanin-DNA copigmentation complex: mutual protection against oxidative damage. Phytochemistry 52(7):1313–1318
  • Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protocols 3(6):1101–1108
  • Seitz C, Eder C, Deiml B, Kellner S, Martens S, Forkmann G (2006) Cloning, functional identification and sequence analysis of flavonoid 30-hydroxylase and flavonoid 30,50-hydroxylase cDNAs reveals independent evolution of flavonoid 30,50-hydroxylase in the Asteraceae family. Plant Mol Biol 61(3):365–381
  • Terrier N, Glissant D, Grimplet J, Barrieu F, Abbal P, Couture C, Ageorges A, Atanassova R, Léon C, Renaudin J-P, Dédaldé-champ F, Romieu C, Delrot S, Hamdi S (2005) Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development. Planta 222(5):832–847
  • Vian MA, Tomao V, Coulomb PO, Lacombe JM, Dangles O (2006) Comparison of the anthocyanin composition during ripening of Syrah grapes grown using organic or conventional agricultural practices. J Agric Food Chem 54(15):5230–5235
  • Wang SP, Okamoto G, Hirano K (1997) Vine growth and fruit development of ‘Pione’ grapes planted in root-restricted buried and raised beds. J Jpn Soc Hortic Sci 66(2):253–259
  • Wang SP, Okamoto G, Hirano K (1998) Effects of rooting-zone restriction on the changes in carbohydrates and nitrogenous compounds in ‘Kyoho’ grapevines during winter dormancy and early shoot growth. J Jpn Soc Hortic Sci 67(4):577–582
  • Wang S, Okamoto G, Hirano K, Lu J, Zhang C (2001) Effects of restricted rooting volume on vine growth and berry development of Kyoho grapevines. Am J Enol Vitic 52(3):248–253
  • Wang B, He J, Duan C, Yu X, Zhu L, Xie Z, Zhang C, Xu W, Wang S (2012) Root restriction affects anthocyanin accumulation and composition in berry skin of Kyoho grape (Vitis vinifera L. 9 Vitis labrusca L.) during ripening. Sci Hortic 137:20–28
  • Webster AD, Atkinson CJ, Vaughan SJ, Lucas AS, Mallling E, Mallling W (1997) Controlling the shoot growth and cropping of sweet cherry trees using root pruning or root restriction techniques. Acta Hortic 451:643–652
  • Wen P-F, Chen J-Y, Kong W-F, Pan Q-H, Wan S-B, Huang W-D (2005) Salicylic acid induced the expression of phenylalanine ammonia-lyase gene in grape berry. Plant Sci (Amsterdam, Neth) 169(5):928–934
  • Wulf LW, Nagel CW (1978) High-pressure liquid chromatographic separation of anthocyanins of Vitis vinifera. Am J Enol Vitic 29(1):42–49
  • Xie ZS, Li B, Forney CF, Xu WP, Wang SP (2009) Changes in sugar content and relative enzyme activity in grape berry in response to root restriction. Sci Hortic 123(1):39–45
  • Yamane T, Jeong ST, Goto-Yamamoto N, Koshita Y, Kobayashi S (2006) Effects of temperature on anthocyanin biosynthesis in grape berry skins. Am J Enol Vitic 57(1):54–59
  • Yang TY, Zhu LN, Wang SP, Gu WJ, Huang DF, Xu WP, Jiang AL, Li SC (2007) Nitrate uptake kinetics of grapevine under root restriction. Sci Hortic 111(4):358–364
  • Zhu L, Wang S, Yang T, Zhang C, Xu W (2006) Vine growth and nitrogen metabolism of ‘Fujiminori’ grapevines in response to root restriction. Sci Hortic 107(2):143–149
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