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2010 | 52 | 2 |

Tytuł artykułu

Cloning and characterization of a pathogenesis-related gene (ThPR10) from Tamarix hispida

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Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A PR10 gene (ThPR10) was cloned from Tamarix hispida and characterized. Real-time RT-PCR was employed to evaluate gene expression levels. ThPR10 was expressed in both leaves and roots of T. hispida under normal growth conditions, and can be highly induced in both leaf and root tissues by abiotic stresses including NaCl, PEG, cold, CdCl2, and ABA (abscisic acid) treatments. Our results indicated that ThPR10 is involved in the abiotic stress response, and regulated by an ABA-dependent signaling pathway. Subsequently, ThPR10 was localized at the subcellular level. The gene was fused with the GFP N-terminal driven by CaMV35S promoter and transiently expressed in onion epidermal cells. This strategy localized the ThPR10 protein to the nucleus of onion epidermal cells, suggesting that the pathogenesis-related proteins play a functional role in the cell nucleus.

Wydawca

-

Rocznik

Tom

52

Numer

2

Opis fizyczny

p.17-25,fig.,ref.

Twórcy

autor
  • Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, People's Republic of China
autor
autor
autor

Bibliografia

  • AGRAWAL GK, RAKWAL R, and JWA NS. 2000. Rice (Oryza sativa L.) OsPR1b gene is phytohormonally regulated in close interaction with light signals. Biochemical andBiophysical Research Communications 278: 290–298.
  • ANDJELKOVIC V, and THOMPSON R. 2006. Changes in gene expression in maize kernel in response to water and saltstress. Plant Cell Reports 25: 71–79.
  • BEAUCHAMP VB, STROMBERG JC, and STUTZ JC. 2005. Interactions between Tamarix ramosissima (saltcedar), Populus fremontii (cottonwood), and mycorrhizal fungi:Effects on seedling growth and plant species coexistence.Plant and Soil 275: 221–231.
  • BREITENEDER H, HOFFMANN-SOMMERGRUBER K, O'RIORDAIN G. SUSANI M, AHORN H, EBNER C, KRAFT D, and SCHEINER O. 1995. Molecular characterization of Api g 1, the major allergen of celery (Apium graveolens), and its immunological and structural relationships to a group of 17-kDatree pollen allergens. European Journal of Biochemistry233: 484–489.
  • CHRISTENSEN AB, CHO BH, NAESBY M, GREGERSEN PL, BRANDT J, MADRIZ-ORDENANA K, COLLINGE DB, and THORDAL-CHRISTENSEN H. 2002. The molecular characterisation of the two barley proteins establishes the novel PR-17 family of athogenesis-related protein. Moloecular Plant Pathology 3: 134–144.
  • COLDITZ F, NYAMSUREN O, NIEHAUS K, EUBEL H, BRAUN HP, and KRAJINSKI F. 2004. Proteomic approach: identification of Medicago truncatula proteins induced in roots after infection with the pathogenic oomycete Aphanomyces euteiches. Plant Molecular Biology 55: 109–120.
  • DING CK, WANG CY, GROSS KC, and SMITH DL. 2002. Jasmonate and salicylate induce the expression ofpathogenesis-related-protein genes and increase resistanceto chilling injury in tomato fruit. Planta 214: 895–901.
  • DUBOS C, and PLOMION C. 2001. Drought differentially affects expression of a PR-10 protein, in needles of maritimepine (Pinus pinaster Ait.) seedlings. Jounal ofExperimental Botany 52: 1143–1144.
  • EL-KEREAMY A, JAYASANKAR S, TAHERI A, ERRAMPALLI D, and PALIYATH G. 2009. Expression analysis of a plum pathogenesisrelated protein 10 (PR10) during brown rot infection.Plant Cell Reports 28: 95–102.
  • EVELIN H, KAPOOR R, and GIRI B. 2009. Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. Ann Bot.104:1263–1280.
  • FUJIMOTO Y, NAGATA R, FUKASAWA H, YANO K, AZUMA M, IIDA A. et al. 1998. Purification and cDNA cloning of cytokine inspecific binding protein from mung bean (Vigna radiata).European Journal of Biochemistry 258: 794–802.
  • GONNEAU M, PAGANT S, BRUN F, and LALOUE M. 2001. Photoaffinity labeling with the cytokinin agonist azido-CPPU of a 34 kDa peptide of the intracellular pathogenesisrelated protein family in the moss Physcomitrellapatens. Plant Molecular Biology 46: 539–548.
  • HMIDA-SAYARI A, COSTA A, LEONE A, JAOUA S, and GARGOURIBOUZID R. 2005. Identification of salt stress-induced transcriptsin potato leaves by cDNA-AFLP. MolecularBiotechnology 30: 31–40.
  • HOFFMANN-SOMMERGRUBER K, VANEK-KREBITZ M, RADAUER C, WEN J, FERREIRA F, SCHEINER O, and BREITENEDER H. 1997. Genomic characterization of members of the Bet v1 family: genes coding for allergens and pathogenesisrelatedproteins share intron positions. Gene 197:91–100.
  • HU X. JIANG M, ZHANG J, ZHANG A, LIN F, and TAN M. 2007. Calcium-calmodulin is required for abscisic acidinducedantioxidant defense and functions bothupstream and downstream of H2O2 production in leavesof maize (Zea mays) plants. New Phytologyst 173:27–38.
  • HUANG W, MA X, WANG Q, GAO Y, XUE Y, NIU X, YU G, and LIU Y. 2008. Significant improvement of stress tolerance intobacco plants by overexpressing a stress-responsivealdehyde dehydrogenase gene from maize (Zea mays).Plant Moecularl Biology 68: 451–463.
  • JELLOULI N, BEN JOUIRA H, SKOURI H, GHORBEL A, GOURGOURI A, and MLIKI A. 2008. Proteomic analysis of Tunisiangrapevine cultivar Razegui under salt stress. PlantPhysiology 165: 471–481.
  • KIEFFER P, DOMMES J, HOFFMANN L, HAUSMAN JF, and RENAUT J. 2008. Quantitative changes in protein expression of cadmium-exposed poplar plants. Proteomics 8: 2514–2530.
  • KIM ST, YU S, KANG YH, KIM SG, KIM JY, KIM SH, and KANG KY. 2008. The rice pathogen-related protein 10 (JIOsPR10)is induced by abiotic and biotic stresses and exhibitsribonuclease activity. Plant Cell Reports 27: 593–603.
  • KOISTINEN KM, HASSINEN VH, GYNTHER PA, LEHESTRANTA SJ, KEINÄNEN SI, KOKKO HI, OKSANEN EJ, TERVAHAUTA AI, AURIOLA S, and KÄRENLAMPI SO. 2002. Birch PR-10c isinduced by factors causing oxidative stress but appearsnot to confer tolerance to these agents. New Phytologyst155: 381–391.
  • KORE-EDA S, CUSHMAN MA, AKSELROD I, BUFFORD D, FREDRICKSON M, CLARK E, and CUSHMAN JC. 2004. Transcript profiling ofsalinity stress responses by large-scale expressedsequence tag analysis in Mesembryanthemum crystallinum.Gene 341: 83–92.
  • LI H, WANG Y, JIANG J, LIU G, GAO C, and YANG C. 2008. Identification of genes responsive to salt stress onTamarix hispida roots. Gene. [Epub ahead of print]
  • LIU X, HUANG B, LIN J, FEI J, CHEN Z, PANG Y, SUN X, and TANG K. 2006. A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic andantimicrobial activity is stress- and pathogen-inducible.Journal of Plant Physiology 163: 546–556.
  • LIU JJ, and EKRAMODDOULLAH AK. 2006. The family 10 of plant pathogenesis-related proteins: Their structure, regulation, and function in response to biotic and abioticstresses. Physiological and Molecular Plant Pathology68: 3–13.
  • LIVAK KJ, and SCHMITTGEN TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the2(Delta Delta C(T)) Method. Methods 25: 402–408.
  • LORENZ WW, SUN F, LIANG C, KOLYCHEV D, WANG H, ZHAO X, CORDONNIER-PRATT MM, PRATT LH, and DEAN JF. 2006.Water stress-responsive genes in loblolly pine (Pinustaeda) roots identified by analyses of expressedsequence tag libraries. Tree Physiology 26: 1–16.
  • MOONS A, PRINSEN E, BAUW G, and VAN MONTAGU M. 1997. Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots. Plant Cell 9: 2243–2259.
  • ODJAKOVA M, and HADJIIVANOVA C. 2001. The complexity of pathogen defense in plants. Plant Physiology 27: 101–109.
  • ONISHI M, TACHI H, KOJIMA T, SHIRAIWA M, and TAKAHARA H. 2006. Molecular cloning and characterization of a novelsalt-inducible gene encoding an acidic isoform of PR-5protein in soybean (Glycine max [L.] Merr). PlantPhysiology and Biochemistry 44: 574–580.
  • PARK CJ, KIM KJ, SHIN R, PARK JM, SHIN YC, and PAEK KH. 2004. Pathogenesis-related protein 10 isolated from hotpepper functions as a ribonuclease in an antiviral pathway.Plant 37: 186–198.
  • RANDEEP R, GANESH KA, and MASAMI Y. 2001. Light-dependent induction of OsPR10 in rice (Oryza sativa L.) seedlings by the global stress signaling molecule jasmonic acid andprotein phosphatase 2A inhibitors. Plant Science 161:469–479.
  • RIGGLEMAN RC, FRISTENSKY B, and HADWIGER LA. 1985. The disease resistance response in pea is associated withincreased mRNAs. Plant Molecular Biology 4: 81–86.
  • SAROWAR S, KIM YJ, KIM EN, KIM KD, HWANG BK, ISLAM R, and SHIN JS. 2005. Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plantsenhances resistance to heavy metal and pathogen stresses.Plant Cell Reports 24: 216–224.
  • SEO PJ, LEE AK, XIANG F, and PARK CM. 2008. Molecular and functional profiling of Arabidopsis pathogenesis-relatedgenes: insights into their roles in salt response ofseed germination. Plant Cell Physiology 49: 334–344.
  • SRIVASTAVA S, EMERY RJ, KUREPIN LV, REID DM, FRISTENSKY B, and KAV NN. 2006. Pea PR10.1 is a ribonuclease and itstransgenic expression elevates cytokinin levels. PlantGrowth Regulation 49: 17–25.
  • TANG DQ, QIAN HM, YU SW, CAOYF, LIAO ZH, ZHAO LX, SUN XF, HUANG DF, and KEXUAN T. 2004. cDNA cloning and char-acterization of a new stress-responsive gene BoRS1 fromBrassica oleracea var. acephala. PhysiologiaPlantarum 121: 578–585.
  • UENO O. 1998. Induction of Kranz Anatomy and C4-like biochemical characteristics in a submerged amphibious plant by abscisic acid. Plant Cell 10: 571–584.
  • VAN LOON LC, PIERPOINT WS, BOLLER TH, and CONEJERO V. 1994. Recommendation for naming plant pathogenesis-related proteins. Plant Molecular Biology Reporter 12: 245–264.
  • VAN LOON LC, REP M, and Pieterse CMJ. 2006. Significance of inducible defense-related proteins in infected plants.Annual Review of Phytopathology 44: 1–28.
  • WANG CS, HUANG JC, and HU JH. 1999. Characterization of two subclasses of PR-10 transcripts in lily anthers andinduction of their genes through separate signal transductionpathways. Plant Molecular Biology 40: 807–814.
  • WANG YC, BO HX, and YANG CP. 2003. A method for rapid isolation of total RNA from Tamarix and Populous euphraticaOliv. Journal of Northeast Forestry University 31:99–100.
  • WISNIEWSKI M, BASSETT C, and ARORA R. 2004. Distribution and partial characterization of seasonally expressed proteinsin different aged shoots and roots of 'Loring' peach(Prunus persica). Tree Physiology 24: 339–345.
  • XIONG L, ISHITANI M, LEE H, and ZHU JK. 2001. The Arabidopsis LOS5/ABA3 Locus Encodes a MolybdenumCofactor Sulfurase and Modulates Cold Stress- andOsmotic Stress-Responsive Gene Expression. Plant Cell13: 2063–2084.
  • YAN QJ, QI XW, JIANG ZQ, YANG SQ, and HAN LJ. 2008. Characterization of a pathogenesis-related class10 protein (PR-10) from Astragalus mongholicus with ribonuclease activity. Plant Physiology and Biochemistry 46: 93–99.
  • YU XM, GRIFFITH M, and WISEMAN SB. 2001. Ethylene induces antifreeze activity in winter rye leaves. Plant Physiology 126: 1132–1140.
  • ZHU B, CHEN TH, and LI PH. 1995. Expression of three osmotin-like protein genes in response to osmotic stressand fungal infection in potato. Plant Molecular Biology28: 17–26.

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