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2009 | 69 | 3 |
Tytuł artykułu

Elucidating the function of CPEB proteins in microglia

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Cytoplasmic Polyadenylation Element Binding proteins (CPEBs) modulate local protein synthesis in neurons crucial for LTP maintenance, memory, and learning. Our recent results indicate they are involved in the progression of epilepsy in a mouse model of temporal lobe epilepsy (TLE). Neuronal proteins regulated by CPEBs include tissue plasminogen activator (tPA), but the primary source of tPA in brain is microglia. CPEB function in glial cells has hardly been investigated. Since microglial tPA is a crucial neurodegenerative factor in TLE, we explore the attractive hypothesis of microglial CPEB involvement in tPA release and, thereby, neurodegeneration following seizures. By single cell RT-PCR we observed CPEB1-4 mRNAs in mouse microglia. This corroborates RT-PCR and immunoblot results, showing abundant CPEB transcript and protein levels in the BV-2 microglial cell line and ES-cells-derived microglia. We use CX3CR1 EGFP/+ knock-in transgenic mice to isolate microglial cells by FACS. In such primary microglia cultures, we will compare CPEB and tPA expression, polyadenylation state of tPA mRNA, and phosphorylation status of CPEBs before and after microglial activation. Finally, we will look at CPEB-mediated control of tPA expression with a luciferase reporter assay. We hope the abovementioned studies to shed a new light on the function of microglial CPEBs and their potential role in neurodegeneration associated with TLE.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
69
Numer
3
Opis fizyczny
p.339
Twórcy
  • Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany
autor
  • Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany
autor
  • Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany
autor
  • Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany
autor
  • Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany
autor
  • Institute of Reconstructive Neurobiology, University Bonn LIFE and BRAIN Center, Bonn, Germany
  • Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany
autor
  • Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany
Bibliografia
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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