Comparison of egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa

• Autorzy: Akhter S. , Rakha B. A. , Andrabi S. M. H. , Ansari M. S.
• Języki publikacji: EN

Abstrakty

EN

The present study was designed to compare egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa. The study was conducted on cauda epididymal spermatozoa from ten slaughtered Sahiwal bulls. Semen retrieved from epididymal cauda of each testes pair was pooled and diluted in tris-citric acid-glycerol extender containing either pigeon, guinea fowl or chicken egg yolk semi colon leading to a final spermatozoal concentration of 30×106 ml-1. Diluted semen was transferred into 0.5 ml straws, cooled to 4°C and equilibrated for 4 h. The straws were kept on liquid nitrogen vapours for 10 min and then plunged into liquid nitrogen for storage. Motility, plasma membrane integrity and spermatozoa morphology (acrosome ridge;head, mid piece and tail) of each diluted semen sample were assessed at 0, 2 and 4 h post-thawing.All indicators applied and acrosomal integrity were the highest (P<0.05) after freezing in extender containing pigeon egg yolk compared to guinea fowl and chicken egg yolk 0, 2 and at 4 h postthaw.Moreover,spermatozoa tail abnormality percentages were also significantly lower (P<0.05) in extender containing pigeon egg yolk compared to guinea fowl and chicken egg yolk at 0, 2 and 4 post-thaw h. In conclusion, pigeon egg yolk used in semen extender improved the post-thaw quality of Sahiwal bull epididymal spermatozoa.

Słowa kluczowe

EN

bull; cattle; cryopreservation; egg yolk; avian species; Sahiwal breed; epididymal spermatozoon; semen

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2011
• Tom: 29
• Numer: 2
• Opis fizyczny: p.131-138,fig.,ref.

Twórcy

autor

Akhter S.

• Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shab Agriculture University, Rawalpindi 46300, Pakistan

autor

Rakha B. A.

autor

Andrabi S. M. H.

autor

Ansari M. S.

Bibliografia

• ANDRABI S.M.H., MAXWELL WM., 2007 – Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Animal Reproduction Science 99, 223-243.
• ANDRABI S.M.H., ANSARI M.S., ULLAH N., ANWAR M., MEHMOOD A., AKHTER S.,2008 – Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Animal Reproduction Science 104, 427-433.
• ANSARI M.S., RAKHA B.A., ANDRABI S.M.H., ULLAH N., AKHTER S., 2010 – Cryopreservation of Sahiwal bull epididymal spermatozoa. Pakistan Journal of Zoology 42, 741-743.
• ANZAR M., GRAHAM E. F., 1993 – Filtration of bovine semen. Development of a sephadex-ion exchange filter. Animal Reproduction Science 31, 187-195.
• ANZAR M., GRAHAM E.F., 1995 – Effect of filtration on post-thaw quality of bull semen.Theriogenology 43, 439-449.
• BATHGATE R., MAXWELL W.M.C., EVANS G., 2006 – Studies on the effects of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality. Reproduction in Domestic Animals 41, 68-73.
• BERGERON A., MANJUNATH P., 2006 – New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Molecular Reproduction and Development 73, 1338-1344.
• BRITO L.F.C., ALBERT D.B., SYLVIE B.G., PAUL L.P., JOHN P.K., 2003 – Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate.Theriogenology 60, 1539-1551.
• CHOI Y., PARK Y., PARIZA M.W., NTAMBI, J.M., 2001– Regulation of stearoyl-CoA desaturase activity by the trans-10, cis-12 isomer of conjugated linoleic acid in HepG2 cells. Biochemical and Biophysical Research Communications 284, 689-693.
• CLULOW J., Maxwell W.M.C., Evans G., Morris L. H. A., 2004 – A comparison between duck and chicken egg yolk for the cryopreservation of stallion spermatozoa. Proc. 15th Int. Cong. Anim.Reprod., Porto Seguro, Brazil, pp. 506 (Abstract).
• DAHLIN A., KHAN U.N., ZAFAR A.H., SALEEM M., CHAUDHRY M.A., PHILIPSSON J.,1998 – Genetic and environmental causes of variation in milk production traits of Sahiwal cattle in Pakistan. Animal Science 66, 307-318.
• GARCIA O., SAHA A., HEMME T., 2003 – Strategy analysis for a two-cow farm in India/Haryana.IFCN Dairy Report. International Farm Comparison Network, Global Farm GbR, Braunschweig,100-101.
• HUMES R., WEBB G., 2006 – Use of chicken or chukar egg yolk with two cryoprotectants for preservation of stallion semen. Animal Reproduction Science 94, 62-63 (abstract).
• JOSHI B.K., SINGH A., GANDHI R.S., 2001 – Performance evaluation, conservation and improvement of Sahiwal cattle in India. Dairy Cattle Breeding Division, National Institute, Karnal, India. AGRI 31, 43-54.
• MANJUNATH P., NAUC V., BERGERON A., MENARD M., 2002 – Major proteins of bovine seminal plasma bind to the low-density lipoproteins fraction of hen’s eggs yolk. Biology of Reproduction 67, 1250-1258.
• MARTINS C.F., RUMPF R., PEREIRA D.C., DODE M.N., 2007 – Cryopreservation of epididymal bovine spermatozoa from dead animals and its uses in in vitro embryo production. Animal Reproduction Science 101, 326-331.
• REHMAN S.U., AHMAD M., SHAFIQ M., 2006 – Comparative performance of Sahiwal cows at the livestock experiments stations Bahadurnagar, Okara vs Patadars herd. Pakistan Veterinary Journal 26, 179-183.
• ROCHA A., OLIVEIRA E., DIAZ J.K., SOUSA M., 2006 – A novel apical midpiece defect in the spermatozoa of a bull without an apparent decrease in motility and fertility: a case study.Theriogenology 66, 913-922.
• SODERQUIST L., JANSON L., LARSSON K., EINARSSON S., 1991 – Sperm morphology and fertility in A.I. bull. Journal of Veterinary Medicine 38, 534-543.
• SU L., LI X., QUAN J., YANG S., LI Y., HE X., TANG X., 2008 – A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm. Animal Reproduction Science 104, 212-219.
• THOMAS C.A., GARNER D.L., JARNETTE J.M., MARSHALL C.E., 1997 – Fluorometric assessment of acrosomal integrity and vialibity in cryopreserved bovine spermatozoa. Biology of Reproduction 45, 880-887.
• TRIMECHE A., ANTON M., RENARD P., GANDEMER G., TAINTURIER D., 1997 – Quail egg yolk: A novel cryoprotectants for the freeze preservation of Poitou jackass sperm. Cryobiology 34,385-393.
• TRIMECHE A., RENARD P., TAINTURIER D., 1998 – A procedure for Poitou jackass sperm cryopreservation, Theriogenology 50, 793-806.