In vitro responses of various explants of Fagus sylvatica

• Autorzy: Hazubska-Przybyl T. , Chmielarz P. , Bojarczuk K.
• Języki publikacji: EN

Abstrakty

EN

There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.

Słowa kluczowe

EN

European beech; plant response; explant; Fagus sylvatica; micropropagation; culture condition; tissue culture; in vitro culture

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2015
• Tom: 73
• Opis fizyczny: p.135-144,fig.,ref.

Twórcy

autor

Hazubska-Przybyl T.

• Institute of Dendrology, Parkowa 5, 62‑035 Kornik, Poland

autor

Chmielarz P.

• Institute of Dendrology, Parkowa 5, 62‑035 Kornik, Poland

autor

Bojarczuk K.

• Institute of Dendrology, Parkowa 5, 62‑035 Kornik, Poland

Bibliografia

• Joergensen J. 1988. Embryogenesis in Quercus petraea and Fagus sylvatica. Journal of Plant Physiology 132: 638–640.
• Kraj W., Dolnicki A. 1998. Odkażanie eksplantatów buka (Fagus sylvatica L.) do kultur tkankowych. Acta Agraria et Silvestria, Series Silvestria 36: 49–62.
• Kraj W., Dolnicki A. 2003. The influence of PPM upon the sterility of the in vitro cultures in European beech. Acta Societatis Botanicorum Poloniae 72: 303–307.
• Lelu-Walter M.A., Paques L., Thompson D., Harvenght L. 2010. Somatic embryogenesis of Forest trees in Europe: What’s going on? An Overview. Proceeding of the IUFRO Working Party 2.09.02. August 19–21.2010: 97–99.
• Lloyd G., McCown B. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Combined Proceedings of the International Plant Propagators Society 30: 421–427.
• Murashige T., Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.
• Naujoks G. 2003. Somatic embryogenesis in beech (Fagus sylvatica). Biologia 58: 83–87.
• San B., Sezgin M., Dumanoglu H., Koksal A.I. 2007. Somatic embryogenesis from immature cotyledons of some European chestnut (Castanea sativa Mill.) cultivars. Turkish Journal of Agriculture and Forestry 31: 175–179.
• Suszka B. 1990. Rozmnażanie wegetatywne. In: Nasze Drzewa Leśne. Monografie popularnonaukowe. Buk zwyczajny. PWN Warszawa-Poznań, pp. 499–524.
• Teixeira da Silva J.A., Malabadi R.B. 2012. Factors affecting somatic embryogenesis in conifers. Journal of Forestry Research 23: 503–515.
• Valladares S., Sánchez C., Martínez M.T., Ballester A., Vieitez A.M. 2006. Plant regeneration through somatic embryogenesis from tissues of mature oak trees : true-to-type conformity of plantlets by RAPD analysis. Plant Cell Reports 25: 879–886.
• Vieitez J.F., Ballester A., Vieitez A.M. 1992. Somatic embryogenesis and plantlet regeneration from cell suspension cultures of Fagus sylvatica L. Plant Cell Reports 11: 609–613.
• Vieitez A.M., Ferro E.M., Ballester A.1993. Micropropagation of Fagus sylvatica L. In Vitro Cellular and Developmental Biology – Plant 29P: 183–188.
• Vieitez A.M., San-José M.C. 1996. Adventitious shoot regeneration from Fagus sylvatica leaf explants in vitro. In Vitro Cellular and Developmental Biology – Plant 32: 140–147.
• Vengadesan G., Pijut P.M. 2009. Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.). Plant Cell, Tissue and Organ Culture 97: 141–149.
• Wilhelm E. 2000. Somatic embryogenesis in oak (Quercus ssp.). In Vitro Cellular Developmental Biology – Plant 36: 349–357.
• Joergensen J. 1988. Embryogenesis in Quercus petraea and Fagus sylvatica. Journal of Plant Physiology 132: 638–640.
• Kraj W., Dolnicki A. 1998. Odkażanie eksplantatów buka (Fagus sylvatica L.) do kultur tkankowych. Acta Agraria et Silvestria, Series Silvestria 36: 49–62.
• Kraj W., Dolnicki A. 2003. The influence of PPM upon the sterility of the in vitro cultures in European beech. Acta Societatis Botanicorum Poloniae 72: 303–307.
• Lelu-Walter M.A., Paques L., Thompson D., Harvenght L. 2010. Somatic embryogenesis of Forest trees in Europe: What’s going on? An Overview. Proceeding of the IUFRO Working Party 2.09.02. August 19–21.2010: 97–99.
• Lloyd G., McCown B. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Combined Proceedings of the International Plant Propagators Society 30: 421–427.
• Murashige T., Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.
• Naujoks G. 2003. Somatic embryogenesis in beech (Fagus sylvatica). Biologia 58: 83–87.
• San B., Sezgin M., Dumanoglu H., Koksal A.I. 2007. Somatic embryogenesis from immature cotyledons of some European chestnut (Castanea sativa Mill.) cultivars. Turkish Journal of Agriculture and Forestry 31: 175–179.
• Suszka B. 1990. Rozmnażanie wegetatywne. In: Nasze Drzewa Leśne. Monografie popularnonaukowe. Buk zwyczajny. PWN Warszawa-Poznań, pp. 499–524.
• Teixeira da Silva J.A., Malabadi R.B. 2012. Factors affecting somatic embryogenesis in conifers. Journal of Forestry Research 23: 503–515.
• Valladares S., Sánchez C., Martínez M.T., Ballester A., Vieitez A.M. 2006. Plant regeneration through somatic embryogenesis from tissues of mature oak trees : true-to-type conformity of plantlets by RAPD analysis. Plant Cell Reports 25: 879–886.
• Vieitez J.F., Ballester A., Vieitez A.M. 1992. Somatic embryogenesis and plantlet regeneration from cell suspension cultures of Fagus sylvatica L. Plant Cell Reports 11: 609–613.
• Vieitez A.M., Ferro E.M., Ballester A.1993. Micropropagation of Fagus sylvatica L. In Vitro Cellular and Developmental Biology – Plant 29P: 183–188.
• Vieitez A.M., San-José M.C. 1996. Adventitious shoot regeneration from Fagus sylvatica leaf explants in vitro. In Vitro Cellular and Developmental Biology – Plant 32: 140–147.
• Vengadesan G., Pijut P.M. 2009. Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.). Plant Cell, Tissue and Organ Culture 97: 141–149.
• Wilhelm E. 2000. Somatic embryogenesis in oak (Quercus ssp.). In Vitro Cellular Developmental Biology – Plant 36: 349–357.

Identyfikatory

DOI

10.12657/denbio.073.014