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2011 | 71 | S |
Tytuł artykułu

The effects of restraint stress on GABAergic and glutamatergic inpusts to the paraventricular nucleus of the rat

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Chronic stress and resulting from it, prolonged hyperactivation of the hypothalamic–pituitary–adrenal (HPA) axis and elevated level of glucocorticoids in the circulatory system, have been linked to the pathophysiology of depressive disorders. Dysregulation of the HPA axix is characteristic for individuals with depression. The paraventricular nucleus of the hypothalamus (PVN) is a major regulator of stress responses acting via the release of corticotropin releasing hormone (CRH) to the pituitary gland. This study was aimed at establishing the effects of restraint stress on GABAergic and glutamatergic inputs to the paraventricular nucleus of the rat. Male adult rats were subjected to restraint in metal tubes lasting for 10 min (2 times daily, repeated for 3 consecutive days). Control animals were kept in home cages. Twenty-four hour after the last stress session rats were decapitated, their brains were removed and slices containing a part of PVN (420 µm thick) were cut using a vibrating microtome. Neurons were visualized using Zeiss Axioskop upright microscope using Nomarski optics, a 40× water immersion lens and an infrared camera parvocellular neurons of PVN were selected based on the morphology of the soma and on the response to depolarizing current pulses. Spontaneous IPSCs were recorded from parvocellular neurons which were voltage clamped at 0 mV and sEPSCs were recorded at -76 mV. The amplitude and the frequency of sIPSCs and sEPSCs were recorded. Obtained data indicate that the restraint stress resulted in an increase in the mean frequency of sEPSCs while the mean amplitude of sEPSCs was not altered. The restraint stress had no effect on frequency and amplitude of sIPSCs. Thus, restraint stress enhances glutamatergic, but not GABAergic, inputs to the paraventricular nucleus of the rat. Grant: POIG 01.01.02-12-004/09-00 financed by European Regional Development Fund.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
71
Numer
S
Opis fizyczny
p.62
Twórcy
autor
  • Department of Physiology, Institute of Pharmacology PAS, Krakow, Poland
autor
  • Department of Physiology, Institute of Pharmacology PAS, Krakow, Poland
autor
  • Department of Physiology, Institute of Pharmacology PAS, Krakow, Poland
  • Department of Physiology, Institute of Pharmacology PAS, Krakow, Poland
autor
  • Department of Physiology, Institute of Pharmacology PAS, Krakow, Poland
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Bibliografia
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bwmeta1.element.agro-9f81855c-5d8c-4b93-b6b9-878b1a70ea6c
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