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2013 | 57 | 1 |

Tytuł artykułu

Elastase, myeloperoxidase, and alkaline phosphatase release and free radical generation in neutrophils isolated from blood of sows at different stages of oestrous cyclee

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was to assess the influence of the oestrous cycle phase on neutrophil secretory activity and to extrapolate it to susceptibility to uterine infections. The obtained results indicate that the highest enzyme release seen in the late follicular phase (elastase release was 42.18 ±3.11% of maximal release, myeloperoxidase was 45.0 ±5.12%, and alkaline phosphatase was 44.75 ±9.0%) was related to the level of 17ß-oestradiol in plasma. Similarly, a free radical generation was also the most enhanced during this phase. Significantly lower values were obtained from sows during the luteal phase in regard to both enzyme release (36.62 ±3.58% for elastase, 27.87 ±8.7% for myeloperoxidase, and 22.12 ±2.4% for alkaline phosphatase), and that of free radicals (2.28 ±1.6 µM/10⁶ cells for nitric oxide and 2.47 ±0.6 nM/10⁶ cells for superoxide).

Słowa kluczowe

Wydawca

-

Rocznik

Tom

57

Numer

1

Opis fizyczny

p.65-68,fig.,ref.

Twórcy

  • Chair of Preclinical Veterinary Sciences, Department of Pathophysiology Faculty of Veterinary Medicine, University of Life Sciences, 20-033 Lublin, Poland
autor
  • Chair of Preclinical Veterinary Sciences, Department of Pathophysiology Faculty of Veterinary Medicine, University of Life Sciences, 20-033 Lublin, Poland

Bibliografia

  • 1. Bekesi G., Kakucs R., Varbiro S., Racz K., Sprintz D., Feher J., Szekacs B.: In vitro effects of different steroid hormones on superoxide anion production of human neutrophil granulocytes. Steroids 2000, 65, 889-894.
  • 2. Bird D., Karavitis J., Kovacs E.: Sex differences and estrogen modulation of the cellular immune response after injury. Cell Immunol 2008, 252, 57-67.
  • 3. Brannstrom M., Enskog A.: Leukocyte networks and ovulation. J Rep Immunol 2002, 57, 47-60.
  • 4. Chung W-B., Cheng W-F., Wu L-S., Yang P-Ch.: The use of plasma progesterone to predict the reproductive status of anestrous gilts and sows. Theriogenology 2002, 58, 1165-1174.
  • 5. De Winter P.J., Verdonck M., de Kruif A., Coryn M., Deluyker H.A., Devriese L.A., Haesebrouck F.: The relationship between the blood progesterone concentration at earh metoestrus and uterine infection in the sow. Anim Reprod Sci 1996, 41, 51-59.
  • 6. Duran M., Galvez G., de Frutos T., Recasens J., Casado S., Farre A.L.: 17 beta-estradiol-stimulated nitric oxide production by neutrophils: effect on platelet activation. Obstet Gynecol 2000, 95, 284-90.
  • 7. Karlsson A., Khalfan L., Dahlgren C., Stigbrand T., Follin P.: Neutrophil alkaline phosphatise activity increase in bacterial infections is not associated with a general increase in secretory vesicle membrane components. Infect Immun 1995, 63, 911-916.
  • 8. Kosior-Korzecka U., Bobowiec R.: Changes in the level of endogenous leptin, FSH, 17ß-oestradiol and metabolites during lupin-induced increase in ovulation rate in ewes. J Vet Med 2003, A50, 343-349.
  • 9. Kowalik M., Rękawiecki R., Kotwica J.: Genomic and non-genomic effect of progesterone on the cells of the female reproductive tract. Med Weter 2008, 64, 528-532.
  • 10. Lewis G.S.: Steroidal regulation of uterine immune defences. Anim Reprod Sci 2004, 82-83, 281-294.
  • 11. Lewis G.S.: Steroidal regulation of uterine resistance to bacterial infection in livestock. Reprod Biol Endocrinol 2003, http://www.rbej.eom/content/l/l/l 17.
  • 12. Magnusson U.: In vitro effects of postpartum concentrations of oestradiol-17 ß on cell-mediated immunity and phagocytosis by porcine leukocytes. Vet Immunol Immunopathol 1991, 28, 117-126.
  • 13. Magnusson U., Einarsson S.: Effects of exogenous oestradiol on the number and functional capacity of circulating mononuclear and polymorphonuclear leukocytes in the sow. Vet Immunol Immunopathol 1990, 25, 235-247.
  • 14. Nims R., Darbyshire J.F., Saavedra J.E., Christodoulou D., Hanbauer I., Cox G.W., Grisham M., Krishna M., Wink D.: Colorimetric methods for the determination of nitric oxide concentration in neutral aqueous solutions. Comp Meth Enzymol 1995, 7, 48-54.
  • 15. Osterlundh I., Holst H., Magnusson U.: Effect of mammary secretions on functions of polymorphonuclear leukocytes in pigs. Am J Vet Res 2001, 62, 1250-1254.
  • 16. Osterlundh I., Hulten F., Johannisson A., Magnusson U.: Sows intramammarily inoculated with Escherichia coli at parturition: I Functional capacity of granulocytes in sows affected or non-affected by clinical mastitis. Vet Immunol Immunopathol 2002, 90, 35-44.
  • 17. Ródenas J., Mitjavila M., Carbonell T.: Simultaneous generation of nitric oxide and superoxide by inflammatory cells in rats. Free Rad Biol Med 1995, 18, 869-875.
  • 18. Roth J.A., Kaeberle M.L., Appell L.H., Nachreiner R.F.: Association of increased estradiol and progesterone blood values with altered bovine polymorphonuclear leukocyte function. Am J Vet Res 1983, 44, 247-253.
  • 19. Wessely-Szponder J.: The influence of TNF and IL-8 on secretan action of neutrophils isolated from heifers in the course of BRD. Acta Vet Hung 2008, 56, 187-196.
  • 20. Wessely-Szponder J., Szponder T.: Comparison of the effects of two anaesthetic combinations in rabbits on some neutrophil functions in vitro. World Rabbit Sci 2010, 18, 169-177.
  • 21. Winters K., Meyer E., van Merris V., van den Broeck W., Duchateau L., Burvenich C.: Sex steroid hormones do not influence the oxidative burst activity of polymorphonuclear leukocxtes from ovariectomised cows in vitro. Steroids 2003, 68, 397-406.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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