Astaxanthin synthesis by Xanthophyllomyces dendrorhous DSM 5626 and its astaxanthin overproducing mutants on xylose media under different illumination

• Autorzy: Stachowiak B.

Warianty tytułu

PL

Synteza astaksantyny przez Xanthophyllomyces dendrorhous DSM 5626 i jego nadprodukujące astaksantynę mutanty na podłożach z ksylozą, przy rożnym natężeniu oświetlenia

• Języki publikacji: EN

Abstrakty

EN

Background. Astaxanthin is the most important and expensive carotenoid pigment used in aquaculture. Its commercial attractiveness is also related with its antioxidant potential. Xanthophyllomyces dendrorhous yeast is considered to be promising for commercial production of astaxanthin. The aim of this study was to investigate the possibility of the growth and astaxanthin production by X. dendrorhous strains on media containing xylose under different illumination. Material and methods. X. dendrorhous DSM 5626 and its mutants: 10BE and 26UV were used in this study. The cultures were carried out on hydrolysed rye stillage (HS) and YM medium with xylose (YM-K). Celi concentration, total carotenoid and astaxanthin yields were assessed in 5-day cultures. The effect of illumination in the range of 0-5,000 lx on growth and on astaxanthin production of yeasts in cultures run on YM-K medium was also examined. Resuits. For the tested yeast strains better growth parameters and astaxanthin yields were obtained on the YM-K medium, on which for all strains the highest pigment yields were recorded at 600-1,000 lx. The highest concentration of astaxanthin in cells was recorded for 26UV in a culture at 1,000 lx (0.51 g kg1 DCW). The volume yield of the pigment regardless of strain was highest in cultures at 600 lx. In this case 10BE was found to be the best astaxanthin producer with a yield of 2.15 mg dm'3. Conclusions. Astaxanthin synthesis in X. dendrorhous DSM 5626 and its mutants was better on YM-K medium comparing to hydrolysed rye stillage. Moreover, carotenogenesis in the studied yeast strains was subjected to marked photoregulation. Illumination within the range of 600-1,000 lx promotes carotenogenesis and astaxanthin production, while exceeding a certain light capacity resuits in microbial celi death.

PL

Wstęp. Astaksantyna jest najważniejszym i najdroższym karotenoidem stosowanym w akwakulturze. Jej komercyjna atrakcyjność wynika również z wysokiego potencjału przeciwutleniającego. Obecnie drożdże Xanthophyllomyces dendrorhous wydają się najbardziej obiecującymi mikroorganizmami w przemysłowej produkcji tego barwnika. Celem pracy było zbadanie możliwości wzrostu i produkcji astaksantyny przez szczepy X. dendrorhous w hodowlach na podłożach z ksylozą oraz zbadanie wpływu oświetlenia na karotenogenezę. Material i metody. W badaniach użyto szczep X. dendrorhous DSM 5626 oraz jego mutanty: 10BE i 26UV. Hodowle przeprowadzono na zhydrolizowanym wywarze żytnim (HS) i na podłożu YM z ksylozą (YM- K). Po pięciu dniach w hodowlach kontrolowano: wydajność biomasy oraz komórkową i objętościową wydajność karotenoidów i astaksantyny. W hodowlach na podłożu YM-K oceniano również wpływ natężenia oświetlenia w zakresie 0-5000 lx na wzrost drożdży i karotenogenezę. Wyniki. Dla badanych szczepów drożdży lepsze parametry wzrostu i wydajności astaksantyny odnotowano w hodowlach na podłożu YM-K niż na HS. W hodowlach na podłożu YM-K najlepsze wydajności astaksantyny uzyskano przy natężeniu oświetlenia 600-1000 lx. Największą komórkową koncentrację astaksantyny odnotowano dla szczepu 26UV w hodowli przy 1000 lx (0,51 g kg'1 DCW), natomiast objętościową dla 10BE, w hodowli przy 600 lx (2,15 mgdm'3). Wnioski. Badane szczepy X. dendrorhous DSM 5626 i jego mutanty rosły lepiej i syntetyzowały więcej astaksantyny na podłożu YM-K niż na zhydrolizowanym wywarze żytnim. Ponadto u badanych drożdży karotenogeneza podlegała silnej fotoregulacji. Natężenie oświetlenia w zakresie 600-1000 lx sprzyjało syntezie karotenoidów, w tym astaksantyny.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Technologia Alimentaria
• Rocznik: 2014
• Tom: 13
• Numer: 3
• Opis fizyczny: p.279-288,ref.

Twórcy

autor

Stachowiak B.

• Institute of Technology Plant Origin Food, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland

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