Expression of VPAC1 receptor at the level of mRNA and protein in the porcine female reproductive system

• Autorzy: Bukowski R. , Wasowicz K.
• Języki publikacji: EN

Abstrakty

EN

The presence and distribution of vasoactive intestinal polypeptide (VIP) receptor VPAC1 was studied in the ovary, oviduct and uterus (uterine horn and cervix) of the domestic pig using methods of molecular biology (RT-PCR and immunoblot) and immunohistochemistry. The expression of VPAC1 receptor at mRNA level was confirmed with RT-PCR in all the studied parts of the porcine female reproductive system by the presence of 525 bp PCR product and at the level of proteins by the detection of 46 kDa protein band in immunoblot. Immunohistochemical stainings revealed the cellular distribution of VPAC1 receptor protein. In the ovary it was present in the wall of arterial blood vessels, as well as in the ovarian follicles of different stages. In the tubular organs the VPAC1 receptor immunohistochemical stainings were observed in the wall of the arterial blood vessels, in the muscular membrane, as well as in the mucosal epithelium. The study confirmed the presence of VPAC1 receptor in the tissues of the porcine female reproductive tract what clearly shows the possibility of influence of VIP on the porcine ovary, oviduct and uterus.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 1
• Opis fizyczny: p.199-206,ref.

Twórcy

autor

Bukowski R.

• Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-957 Olsztyn, Poland

autor

Wasowicz K.

• Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Faculty of Veterinary Medicine, University of Warmia and Mazury in OLsztyn, Oczapowskiego 13, 10-957 Olsztyn, Poland

Bibliografia

• Abad C, Martinez C, Juarranz MG, Arranz A, Leceta J, Delgado M, Gomariz RP (2003) Therapeutic effects of vasoactive intestinal peptide in the trinitrobenzene sulfonic acid mice model of Crohn's disease. Gastroenterology 124: 961-971.
• Altiere RJ, Diamond L (1983) Relaxant effects of vasoactive intestinal peptide and bethanechol in cat intrapulmonary artery. Eur J Pharmacol 93: 121-124.
• Arciszewski MB, Ekblad E (2005) Effects of vasoactive intestinal polypeptide and galanin on survival of cultured porcine myenteric neurons. Reg Peptides 125: 185-192.
• Arciszewski MB, Sand E, Ekblad E (2008) Vasoactive intestinal peptide rescues cultured rat myenteric neurons from lipopolysaccharide induced cell death. Reg Peptides 146: 218-223.
• Assil IQ, Abou-Samra AB (2001) N-glycosylation of CRF receptor type 1 is important for its ligand-specific interaction. Am J Physiol Endocrinol Metab 281: E1015-E1021.
• Bajo AM, Guijarro LG, Juarranz MG, Valenzuela P, Martinez P, Prieto JC (1993a) Stimulation of the adenylyl cyc-lase activity in human endometrial membranes by VIP and related peptides. Biosci Rep 13: 69-77.
• Bajo AM, Guijarro LG, Juarranz MG, Valenzuela P, Martinez P, Prieto JC (1993b) Stimulation of the adenylyl cyclase activity in human endometrial membranes by VIP and related peptides. Biosci Rep 13: 69-77.
• Bajo AM, Juarranz MG, Valenzuela P, Martinez P, Prieto JC, Guijarro LG (2000) Expression of vasoactive intestinal peptide (VIP) receptors in human uterus. Peptides 21: 1383-1388.
• Barnes PJ, Cadieux A, Carstairs JR, Greenberg B, Polak JM, Rhoden K (1986) Vasoactive intestinal peptide in bovine pulmonary artery: localisation, function and receptor autoradiography. Br J Pharmacol 89: 157-162.
• Bredkjoer HE, Palle C, Ekblad E, Fahrenkrug J, Ottesen B (1997) PreproVIP-derived peptides in the human female genital tract: expression and biological function. Neuropeptides 31: 209-215.
• Candenas ML, Magraner J, Armesto CP, Anselmi E, Nieto PM, Martin JD, Advenier C, Pinto FM (2001) Changes in the expression of tachykinin receptors in the rat uterus during the course of pregnancy. Biol Reprod 65: 538-543.
• Cecconi S, Rossi G, Barberi M, Scaldaferri L, Canipari R (2004) Effect of pituitary adenylate cyclase-activating polypeptide and vasoactive intestinal polypeptide on mouse preantral follicle development in vitro. Endocrinology 145: 2071-2079.
• Chen Y, Chen Q, Lu G, Fan Z, Zhong S (1994) The autocrine regulatory effect of vasoactive intestinal peptide on the growth of human pancreatic carcinoma cells. Chin Med Sci J 9: 215-219.
• Davoren JB, Hsueh AJ (1985) Vasoactive intestinal peptide: a novel stimulator of steroidogenesis by cultured rat granulosa cells. Biol Reprod 33: 37-52.
• Drews J (2000) Drug discovery: a historical perspective. Science 287: 1960-1964.
• Fahrenkrug J, Ottesen B, Palle C (1988) Vasoactive intestinal polypeptide and the reproductive system. Ann N Y Acad Sci 527: 393-404.
• Ganea D (1996) Regulatory effects of vasoactive intestinal peptide on cytokine production in central and peripheral lymphoid organs. Adv Neuroimmunol 6: 61-74.
• Garcia-Fernandez MO, Solano RM, Carmena MJ, Busto R, Bodega G, Ruiz-Villaespesa A, Prieto JC, Sanchez-Chapado M (2003) Expression of functional PACAP/VIP receptors in human prostate cancer and healthy tissue. Peptides 24: 893-902.
• Gkonos PJ, Guo F, Burnstein KL (2000) Type 1 vasoactive intestinal peptide receptor expression in PC3/AR cells is evidence of prostate epithelial differentiation. Prostate 42: 137-144.
• Goto T, Yamaza T, Kido MA, Tanaka T (1998) Light- and electron-microscopic study of the distribution of axons containing substance P and the localization of neurokinin-1 receptor in bone. Cell Tissue Res 293: 87-93.
• Happola O, Lakomy M (1989) Immunohistochemical localization of calcitonin gene-related peptide and bombesin/gastrin-releasing peptide in nerve fibers of the rat, guinea pig and pig female genital organs. Histochemistry 92: 211-218.
• Happola O, Lakomy M, Majewski M, Yanaihara N (1991) Distribution of somatostatin- and neuropeptide Y-im-munoreactive nerve fibers in the porcine female reproductive system. Neurosci Lett 122: 273-276.
• Happola O, Lakomy M, Yanaihara N (1989) Met5-enkephalin- and Met5-enkephalin-Arg6-Gly7-Leu8-im-munoreactive nerve fibers in the pig female reproductive system. Neurosci Lett 101: 156-162.
• Hokfelt T, Bartfai T, Bloom F (2003) Neuropeptides: opportunities for drug discovery. Lancet Neurol 2: 463-472.
• Holmes A, Heilig M, Rupniak NM, Steckler T, Griebel G (2003) Neuropeptide systems as novel therapeutic targets for depression and anxiety disorders. Trends Pharmacol Sci 24: 580-588.
• Ikeda K, Inoue S (2004) Estrogen receptors and their downstream targets in cancer. Arch Histol Cytol 67: 435-442.
• Jovanovic A, Jovanovic S, Tulic I, Grbovic L (1998) Predominant role for nitric oxide in the relaxation induced by vasoactive intestinal polypeptide in human uterine artery. Mol Hum Reprod 4: 71-76.
• Kawashima M, Takahashi T, Yasuoka T, Kamiyoshi M, Tanaka K (1995) A vasoactive intestinal peptide binding component in hen granulosa cells. Proc Soc Exp Biol Med 209: 387-391.
• Laburthe M, Couvineau A, Marie JC (2002) VPAC receptors for VIP and PACAP. Receptors Channels 8: 137-153.
• Langley JN (1901) Observations on the physiological action of extracts of the suprarenal bodies. J Physiol 17: 231-256.
• Leceta J, Gomariz RP, Martinez C, Abad C, Ganea D, De-lgado M (2000) Receptors and transcriptional factors involved in the anti-inflammatory activity of VIP and PACAP. Ann N Y Acad Sci 921: 92-102.
• Lilling G, Wollman Y, Goldstein MN, Rubinraut S, Fridkin M, Brenneman DE, Gozes I (1994) Inhibition of human neuroblastoma growth by a specific VIP antagonist. J Mol Neurosci 5: 231-239.
• Majewski M, Sienkiewicz W, Kaleczyc J, Mayer B, Czaja K, Lakomy M (1995) The distribution and co-localization of immunoreactivity to nitric oxide synthase, vasoactive intestinal polypeptide and substance P within nerve fibres supplying bovine and porcine female genital organs. Cell Tissue Res 281: 445-464.
• Marvizon JC, Martinez V, Grady EF, Bunnett NW, Mayer EA (1997) Neurokinin 1 receptor internalization in spinal cord slices induced by dorsal root stimulation is mediated by NMDA receptors. J Neurosci 17: 8129-8136.
• Ottesen B (1981) Vasoactive intestinal polypeptide (VIP): effect on rabbit uterine smooth muscle in vivo and in vitro. Acta Physiol Scand 113: 193-199.
• Ottesen B, Gerstenberg T, Ulrichsen H, Manthorpe T, Fahrenkrug J, Wagner G (1983) Vasoactive intestinal poly-peptide (VIP) increases vaginal blood flow and inhibits uterine smooth muscle activity in women. Eur J Clin Invest 13: 321-324.
• Ottesen B, Larsen JJ, Staun-Olsen P, Gammeltoft S, Fahrenkrug J (1985) Influence of pregnancy and sex steroids on concentration, motor effect and receptor binding of VIP in the rabbit female genital tract. Regul Pept 11: 83-92.
• Owman C, Stjernquist M (2003) Origin, distribution and functional aspects of aminergic and peptidergic nerves in the male and female reproductive tracts. In: Bjorklund A, Hokfelt T, Owman C (eds) The Peripheral Nervous System. vol 6. Handbook of Chemical Neuroanatomy. Elsevier Science Publishers B.V., Amsterdam, pp. 445-544.
• Owman C, Stjernquist M, Helm G, Kannisto P, Sjoberg NO, Sundler F (1986) Comparative histochemical distribution of nerve fibres storing noradrenaline and neuropeptide Y (NPY) in human ovary, fallopian tube, and uterus. Med Biol 64: 57-65.
• Pozo D, Guerrero JM, Calvo JR (2000) Functional and molecular characterization of VIP receptor-effector system in rat developing immunocompetent cells: G protein involvement. J Neuroimmunol 103: 41-50.
• Reubi JC (2000) In vitro evaluation of VIP/PACAP receptors in healthy and diseased human tissues. Clinical implications. Ann N Y Acad Sci 921: 1-25.
• Said SI, Dickman K, Dey RD, Bandyopadhyay A, De Stefanis P, Raza S, Pakbaz H, Berisha HI (1998) Glutamate toxicity in the lung and neuronal cells: prevention or attenuation by VIP and PACAP. Ann N Y Acad Sci 865: 226-237.
• Sobel A (1977) Large-scale purification of the acetylcholine-receptor protein in its membrane-bound and detergent-extracted forms from Torpedo marmorata electric organ. Eur J Biochem 80: 215-224.
• Swindle MM, Moody DC, Philips LD (1992) Swine as a model in biomedical research. Iowa State University Press, Ames.
• Vertongen P, Schiffmann SN, Gourlet P, Robberecht P (1997) Autoradiographic visualization of the receptor subclasses for vasoactive intestinal polypeptide (VIP) in rat brain. Peptides 18: 1547-1554.
• Waschek JA (1995) Vasoactive intestinal peptide: an important trophic factor and developmental regulator? Dev Neurosci 17: 1-7.
• Waschek JA, Richards ML, Bravo DT (1995) Differential expression of VIP/PACAP receptor genes in breast, intestinal, and pancreatic cell lines. Cancer Lett 92: 143-149.
• Wasowicz K (2003) Effect of total or partial uterus extirpation on uterus-projecting neurons in porcine inferior mesenteric ganglion. A. Changes in the expression of transmitter-synthesizing enzymes - tyrosine hydroxylase, dopamine beta-hydroxylase and choline acetyltran-sferase. Pol J Vet Sci 6: 131-145.
• Wasowicz K, Kaleczyc J, Majewski M, Lakomy M, Ziecik A, Kraeling R (1998) Influence of oestradiol on distribution of VIP- and NPY-positive nerve fibers and VIP and NPY concentrations in the porcine female reproductive tract. Pol J Vet Sci 1: 39-46.
• Wasowicz K, Majewski M, Kaleczyc J, Lakomy M, Ziecik A, Kraeling R (1999) NPY and VIP innervation of the oviduct and uterus of the pig at different stages of the oestrus cycle. Pol J Vet Sci 2: 137-143.
• Zimmerman RP, Gates TS, Mantyh CR, Vigna SR, Welton ML, Passaro EP Jr, Mantyh PW (1989) Vasoactive intestinal polypeptide receptor binding sites in the human gastrointestinal tract: localization by autoradiography. Neur-oscience 31: 771-783.

Identyfikatory

DOI

10.1515/pjvs-2015-002