Changes of DNA methylation and hydroxymethylation in plant prptoplast culture

• Autorzy: Moricova P. , Ondrej V. , Navratilova B. , Luhova L.
• Języki publikacji: EN

Abstrakty

EN

 Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found that 5-hmC did not primarily arise as a product of oxidative DNA damage following protoplast culture.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 1
• Opis fizyczny: p.33-36,fig.,ref.

Twórcy

autor

Moricova P.

• Department of Biochemistry and Department of Botany, Faculty of Science, Palacký University, Olomouc, Czech Republic

autor

Ondrej V.

• Department of Biochemistry and Department of Botany, Faculty of Science, Palacký University, Olomouc, Czech Republic

autor

Navratilova B.

• Department of Biochemistry and Department of Botany, Faculty of Science, Palacký University, Olomouc, Czech Republic

autor

Luhova L.

• Department of Biochemistry and Department of Botany, Faculty of Science, Palacký University, Olomouc, Czech Republic

Bibliografia

• Avramova ZJ (2002) Heterochromatin in animals and plants. Similarities and differencies. Plant Physiol 129: 40-49. 
• Exner V, Hennig L (2008) Chromatin rearrangements in development. Curr Opin Plant Biol 11: 64-69. 
• Gajdová J, Navrátilová B, Smolná J, Lebeda A (2007) Factors affecting protoplast isolation and cultivation of Cucumis spp. J Appl Bot Food Qual 81: 1-6.
• Grigoryev SA, Bulynko YA, Popova EY (2006) The end adjusts the means: heterochromatin remodeling during terminal cell differentiation. Chromosome Res 14: 53-69. 
• Kriaucionis S, Heintz N (2009) The Nuclear DNA Base 5-Hydroxymethylcytosine Is Present in Purkinje Neurons and the Brain. Science 324: 929-930. 
• Navrátilová B, Bůžek J, Široký J, Havránek P (1997) Construction of intergeneric somatic hybrids between Brassica oleracea and Armoracia rusticana. Biol Plant 39: 531-541.
• Nestor CE, Ottaviano R, Reddington J, Sproul D, Reinhardt D, Dunican D, Katz E, Dixon JM, Harrison DJ, Meehan RR (2012) Tissue type is a major modifier of the 5-hydroxymethylcytosine content of human genes. Genome Res 22: 467-477. 
• Ondrej V, Kitner M, Dolezalova I, Nadvornik P, Navratilova B, Lebeda A (2009b) Chromatin structural rearrangement during dedifferentiation of protoplasts of Cucumis sativus L. Mol Cells 27: 443-447. 
• Ondrej V, Navratilova B, Lebeda A (2009a) Heterochromatin as a marker for protoplast differentiation of Cucumis sativus. Plant Cell Tiss Org 96: 229-234.
• Ondrej V, Navratilova B, Protivankova I, Piterkova J, Sedlarova M, Luhova L, Lebeda A (2010) Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress. J Exp Bot 61: 2395-2401. 
• Papadakis AK, Roubelakis-Angelakis KA (2002) Oxidative stress could be responsible for the recalcitrance of plant protoplasts. Plant Physiol Biochem 40: 549-559.
• Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rousselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191: 244-250.
• Tahiliani M, Koh PK, Shen Y et al. (2009) Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1. Science 324: 930-935. 
• Tsukada Y (2012) Hydroxylation mediates chromatin demethylation. J Biochem 151: 229-246. 
• Valinluck V, Tsai HH, Rogstad DK et al. (2004) Oxidative damage to methyl-CpG sequences inhibits the binding of the methyl-CpG binding domain (MBD) of methyl-CpG binding protein 2 (MeCP2). Nucl Acids Res 32: 4100-4108. 
• Wu H, Zhang Y (2011) Mechanisms and functions of Tet protein-mediated 5-methylcytosine oxidation. Genes Dev 25: 2436-2452.

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