Biological control of the latent pathogen Gnomoniopsis smithogylvyi in European chestnut grafting scions using Bacillus amyloliquefaciens and Trichoderma atroviride

• Autorzy: Pasche S. , Crovadore J. , Pelleteret P. , Jermini M. , Mauch-Mani B. , Oszako T. , Lefort F.
• Języki publikacji: EN

Abstrakty

EN

A search for endophytes in Castanea sativa Miller (Fagales: Fagaceae) grafting scions showed that a latent pathogenic fungus Gnomoniopsis smithogilvyi (Diaporthales: Gnomoniaceae) was present as the major component of the endophytic flora. Initially, the goal of this study was to develop a biological control method of Cryphonectria parasitica (Diaporthales: Valsaceae), the chestnut blight agent, by soaking chestnut scions before grafting in antagonists suspension. However, the healthy chestnut material used in in vitro and glasshouse experiments turned out to be naturally infected by a pathogen. At first view, the symptoms looked very similar to those caused by C. parasitica but some differences were noticed. DNA sequencing and application of Koch’s postulates revealed that G. smithogilvyi was the agent responsible of those symptoms. Preventive biocontrol experiments were carried out with chestnut tree scions soaked overnight in a liquid suspension of Bacillus amyloliquefaciens (Bacillales: Bacillaceae). This bacterium was then frequently found in the lower parts of scions (CF of 100% between 3.1 and 6 cm) and up to a height of 18 cm. It was observed that when B. amyloliquefaciens was present, the endophytic and opportunistic pathogenic fungus G. smithogilvyi was not present. Conversely, the parts not colonized by the bacteria were always naturally infected by the endophytic fungus. This would indicate that the endophytic behavior of B. amyloliquefaciens inhibited the growth of G. smithogilvyi and reduced its presence in scions. A similar experiment, carried out with the Trichoderma atroviride (Hypocreales: Hypocreaceae), led to similar observations. Trichoderma atroviride was frequently isolated in the lower parts of scions (CF of 100% until 6 cm) and up to a height of 27 cm. Inoculating B. amyloliquefaciens and T. atroviride as part of a preventive biocontrol treatment would allow these biological control agents to colonize the plant as endophytes and prevent the development of G. smithogilvyi.

Słowa kluczowe

EN

biological control; pathogen; Gnomoniopsis smithogylvyi; European chestnut; endophyte; Bacillus amyloliquefaciens; Trichoderma atroviride; chestnut

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2016
• Tom: 75
• Opis fizyczny: p.113-122,fig.,ref.

Twórcy

autor

Pasche S.

• Plants and Pathogens Group, Research Institute Land Nature and Environment, hepia, HES-SO University of Applied Sciences and Arts Western Switzerland, Route de Presinge 150, 1254 Jussy, Geneva, Switzerland

autor

Crovadore J.

• Plants and Pathogens Group, Research Institute Land Nature and Environment, hepia, HES-SO University of Applied Sciences and Arts Western Switzerland, Route de Presinge 150, 1254 Jussy, Geneva, Switzerland

autor

Pelleteret P.

• Plants and Pathogens Group, Research Institute Land Nature and Environment, hepia, HES-SO University of Applied Sciences and Arts Western Switzerland, Route de Presinge 150, 1254 Jussy, Geneva, Switzerland

autor

Jermini M.

• Agroscope, Cadenazzo Research Centre, A Ramel 18, 6593 Cadenazzo, Switzerland

autor

Mauch-Mani B.

• Laboratory of Molecular and Cell Biology, Institute of Biology, Faculty of Sciences, University of Neuchatel, Rue Emile Argand 11, 2000 Neuchatel, Switzerland

autor

Oszako T.

• Department of Forest Protection, Forest Research Institute, 05-090 Sekocin Stary, Poland
• Technical University in Bialystok, Forest Faculty in Hajnowka, Pilsudskiego 8, 17-200 Hajnowka, Poland

autor

Lefort F.

• Plants and Pathogens Group, Research Institute Land Nature and Environment, hepia, HES-SO University of Applied Sciences and Arts Western Switzerland, Route de Presinge 150, 1254 Jussy, Geneva, Switzerland

Bibliografia

• Anonymous (2005) Végétal : Châtaignier (Castanea sp.) Détection de Cryphonectria parasitica sur branches et/ou troncs par observation microscopique, mise en chambre humide et/ou isolement. Laboratoire national de la protection des végétaux, Unité de mycologie forestière et agricole, Ministère de l’agriculture et de la pêche, Malzeville, France
• Arisan-Atac I, Heidenreich E & Kubicek CP (1995) Randomly amplified polymorphic DNA fingerprinting identifies subgroups of Trichoderma viride and other Trichoderma sp. capable of chestnut blight biocontrol. FEMS Microbiology Letters 126: 249–256.
• Baar D & Lecomte M (2011) Bleu coton au lactophénol. Cercle Mycologique de Namur. http://www.champignons-passion.be/BleuCoton2011.pdf, accessed 7 January 2016.
• Bacon CW & Hinton DM (2002) Endophytic and biological control potential of Bacillus mojavensis and related species. Biological Control 23: 274 –284 doi:10.1006/bcon.2001.101.
• Brunner K, Zeilinger S, Ciliento R, Woo SL, Lorito M & Kubicek CP, Mach RL (2005) Improvement of the fungal biocontrol Agent Trichoderma atroviride to enhance both antagonism and induction of plant systemic disease resistance. Applied Environmental Microbiology 71(7): 3959–3965 doi: 10.1128/AEM.71.7.3959-3965.2005.
• Dar MA & Rai MK (2015) Gnomoniopsis smithogilvyi a canker causing pathogen on Castanea sativa: First report. Mycosphere 6(3): 327–336 doi : 10.5943/mycosphere/6/3/8.
• Gagliardi JV, Kough JL, Tomimatsu G, Borges S, Reilly SK & Cerelli S (2011) Bacillus amyloliquefaciens strain D747. Biopesticides registration action document. U.S. Environmental Protection Agency, Office of Pesticide Programs, Biopesticides and Pollution Prevention Division.
• Hanada RE, Jorge Souza TD, Pomella AW, Hebbar KP, Pereira JO, Ismaiel A & Samuels GJ (2008) Trichoderma martiale sp. nov., a new endophyte from sapwood of Theobroma cacao with a potential for biological control. Mycological Research 112: 1335–1343.
• Harman GE, Howell CR, Viterbo A & Chet I (2004) Trichoderma species - opportunistic avirulent plant symbionts. Nature Reviews 2: 43–56.
• Heiniger U (1997) Le chancre de l’écorce du châtaignier (Cryphonectria parasitica). Symptômes et biologie. (2ème édition) Notice pour le Praticien 22 :1–8.
• Howell CR (2003) Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Disease 87(1): 4–10.
• Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML & Pace NR (1985) Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proceedings of the National Academy of Sciences of the USA 82: 6955–6959.
• Lefort F & Douglas GC (1999) An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus. Annals of Forest Science 56: 259–263 doi: 10.1051/forest:19990308.
• Lefort F, Calmin G, Pelleteret P, Farinelli L, Osteras M & Crovadore J (2014) Whole-genome shotgun sequence of Bacillus amyloliquefaciens strain UASWS BA1, a bacterium antagonistic to plant pathogenic fungi. Genome Announcements 2(1): e00016-14. doi:10.1128/genomeA.00016-14.
• Muyzer G, de Waal EC & Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology 59: 695–700.
• Pasche S, Calmin G, Auderset G, Crovadore J, Pelleteret P, Mauch-Mani B, Barja F, Paul B, Jermini M & Lefort F (2016) Assessment of chestnut canker symptoms showed unexpected evidence for Gnomoniopsis smithogilvyi prevalence in Castanea sativa shoots in Switzerland. Fungal Genetics and Biology 97: 9–21 doi:10.1016/j.fgb.2016.01.002.
• Porras-Alfaro A & Bayman P (2011) Hidden fungi, emergent properties: endophytes and microbiomes. Annual Review of Phytopathology 49: 291–315 doi: 10.1146/annurev-phyto-080508-081831.
• Priest FG, Goodfellow M, Shute, LA & Berkeley RCW (1987) Bacillus amyloliquefaciens sp. nov., nom. rev. International Journal of Systematic Bacteriology 37(1): 69–71.
• Ryan BF, Joiner BL & Cryer J (2013) Minitab Handbook: Updated for release 16, 6th edn, Brooks/Cole, Kentucky, USA.
• Schulz B & Boyle C (2005) The endophytic continuum. Mycological Research 109(6): 661–686 doi:10.1017/S095375620500273X.
• Shoresh M, Harman G & Mastouri, F (2010) Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review of Phytopathology 48:21–43. doi:10.1146/annurev-phyto-073009-114450.
• Shuttleworth LA, Guest DI, Liew ECY (2012a) Fungal Planet Description Sheet 107: Gnomoniopsis smithogilvyi. Persoonia 28:142–143.
• Shuttleworth LA, Liew ECY & Guest DI (2012b) Survey of the incidence of chestnut rot in south-eastern Australia. Australasian Plant Pathology 42(1):63–72 doi: 10.1007/s13313-012-0170-2.
• Sunayana N & Prakash HS (2012) Fungal endophytes of Boswellia serrata Roxb. (Burseraceae), a medicinal tree species. International Journal of Pharmacy and Biological Sciences 1(6): 1–5.
• Visentin I, Gentile S, Valentino D, Gonthier P, Tamietti G & Cardinale F (2012) Gnomoniopsis castanea sp. nov (Gnomoniaceae, Diaporthales) as a causal agent of nut rot in sweet chestnut. Journal of Plant Pathology 94: 411–419.
• Wang × & Liang G (2014) Control efficacy of an endophytic Bacillus amyloliquefaciens strain BZ6-1 against peanut bacterial wilt, Ralstonia solanacearum BioMed Research International Article ID 465435 doi:10.1155/2014/465435.
• White JF Jr, Torres MS, Sullivan RF, Jabbour RE, Chen Q, Tadych M, Irizarry I, Bergen MS, Havkin-Frenkel D & Belanger FC (2014) Occurrence of Bacillus amyloliquefaciens as a systemic endophyte of vanilla orchids. Microscopy Research and Technique 77(11):874–85 doi:10.1002/jemt.22410.
• White TJ, Bruns T, Lee J & Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics: PCR protocols, a guide to methods and applications. (ed. by MA Innis, DH Gelfand, JJ Sninsky & TJ White) Academic Press, San Diego, CA, USA, pp. 315–322.
• Wong JH, Hao J, Cao Z, Qiao M, Xu H, Bai Y & Ng TB (2008) An antifungal protein from Bacillus amyloliquefaciens. Journal of Applied Microbiology 105:1888–1898 doi:10.1111/j.1365-2672.2008.03917.x.

Identyfikatory

DOI

10.12657/denbio.075.011