Excretory/secretory products of Fasciola hepatica but not recombinant phosphoglycerate kinase induce death of human hepatocyte cells

• Autorzy: Baska P. , Norbury L. J. , Wisniewski M. , Januszkiewicz K. , Wedrychowicz H.
• Języki publikacji: EN

Abstrakty

EN

The liver fluke Fasciola hepatica infects a wide range of hosts, and has a considerable impact on the agriculture industry, mainly through infections of sheep and cattle. Further, human infection is now considered of public health importance and is hyperendemic in some regions. The fluke infection causes considerable damage to the hosts’ liver. However, the mechanisms of liver destruction have not yet been completely elucidated. In the present report we incubated a human liver cell line in the presence of either F. hepatica excretory/secretory material (FhES) or recombinant phosphoglycerate kinase (FhPGK). Dosedependent cytotoxicity in the presence of FhES was observed, indicating that FhES is capable of killing human hepatocytes, supporting a role for FhES in damaging host liver cells during infection; while treatment with a recombinant intracellular protein — FhPGK, had no impact on cell survival.

Słowa kluczowe

EN

secretory product; excretory product; Fasciola hepatica; liver fluke; infection; sheep; cattle; recombinant phosphoglycerate kinase; cytotoxicity; human hepatocyte

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 2
• Opis fizyczny: p.215-217,fig.,ref.

Twórcy

autor

Baska P.

• Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, 8 Ciszewskiego Street, 02-786 Warsaw, Poland

autor

Norbury L. J.

• Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818 Warsaw, Poland

autor

Wisniewski M.

• Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, 8 Ciszewskiego Street, 02-786 Warsaw, Poland

autor

Januszkiewicz K.

• Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818 Warsaw, Poland

autor

Wedrychowicz H.

• Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818 Warsaw, Poland

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