Anticonvulsant effects of selected antiepileptic drugs in mice exposed to caffeine during pregnancy and breastfeeding in the maximal electroshock-induced seizure model

• Autorzy: Miziak B. , Radzik I. , Czuczwar S.J.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: Caffeine presence in coffee, tea and some drugs makes it the most commonly consumed stimulant drug. Available data show that consumption of caffeine during pregnancy and in the early postpartum period while breastfeeding can affect the offspring’s behaviour at the later stages of life. AIM(S): The aim was to test the impact of caffeine fed to pregnant and breastfeeding mice on the anticonvulsant activity of selected antiepileptic drugs (AEDs): sodium valproate (VPA), topiramate (TPM) and carbamazepine (CBZ), against the maximal electroshock-induced seizures (MES). METHOD(S): From the 3 day after fertilization, pregnant mice received caffeine dissolved in drinking water throughout the pregnancy, and then throughout breastfeeding. The experiments were carried out on adult offspring (mice in their 8th week of age). The control groups comprised mice, born by females that received only pure water throughout the pregnancy and then throughout breastfeeding while experimental groups – mice, born by females that drank caffeine (0.3 g/l). Seizures were induced by alternating current (25 mA, 50 Hz, stimulus duration 0.2 s) delivered by a generator via ear electrodes. Undesirable effects of the combined treatments were examined in the chimney test, passive avoidance task, and grip strength test. RESULTS: In the groups exposed to caffeine, there was a significant impairment of the anticonvulsant action of both, VPA and CBZ. The respective ED50s were increased from 155.5 (146.2–165.4) to 175.5 (164.1–187.7) and from 8.4 (6.9–10.3) to 11.6 (10.5–12.9) mg/kg, respectively. As regards TPM, no significant impact of exposure to caffeine on the effectiveness of this AED has been observed. The neurotoxic effects of AEDs were not affected by caffeine exposure. CONCLUSIONS: The experiments show that exposure to caffeine has a statistically relevant influence on the anticonvulsant action of VPA and CBZ against MES test carried out in the exposed offspring. FINANCIAL SUPPORT: DS 475.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.65

Twórcy

autor

Miziak B.

• Department of Pathophysiology, Medical University of Lublin, Poland

autor

Radzik I.

• Department of Pathophysiology, Medical University of Lublin, Poland

autor

Czuczwar S.J.

• Department of Pathophysiology, Medical University of Lublin, Poland