Dehydrogenase activity of forest soils depends on the assay used

• Autorzy: Januszek K. , Blonska E. , Dluga J. , Socha J.
• Języki publikacji: EN

Abstrakty

EN

Dehydrogenases are exclusively intracellular enzymes, which play an important role in the initial stages of oxidation of soil organic matter. One of the most frequently used methods to estimate dehydrogenase activity in soil is based on the use of triphenyltetrazolium chloride as an artificial electron acceptor. The purpose of this study was to compare the activity of dehydrogenases of forest soils with varied physicochemical properties using different triphenyltetrazolium chloride assays. The determination was carried out using the original procedure by Casida et al., a modification of the procedure which involves the use of Ca(OH)2 instead of CaCO3, the Thalmann method, and the assay by Casida et al. without addition of buffer or any salt. Soil dehydrogenase activity depended on the assay used. Dehydrogenase determined by the Casida et al. method without addition of buffer or any salt correlated with the pH values of soils. The autoclaved strongly acidic samples of control soils showed high concentrations of triphenylformazan, probably due to chemical reduction of triphenyltetrazolium chloride. There is, therefore, a need for a sterilization method other than autoclaving, ie a process that results in significant changes in soil properties, thus helping to increase the chemical reduction of triphenyltetrazolium chloride.

Słowa kluczowe

EN

dehydrogenase activity; forest soil; enzyme; soil enzyme; soil organic matter; triphenyltetrazolium chloride; chemical reduction

• Wydawca: -
• Czasopismo: International Agrophysics
• Rocznik: 2015
• Tom: 29
• Numer: 1
• Opis fizyczny: p.47-59,fig.,ref.

Twórcy

autor

Januszek K.

• Department of Forest Soil Science, University of Agriculture in Krakow, 29 Listopada 46, 31-425 Krakow, Poland

autor

Blonska E.

• Department of Forest Soil Science, University of Agriculture in Krakow, 29 Listopada 46, 31-425 Krakow, Poland

autor

Dluga J.

• Department of Forest Soil Science, University of Agriculture in Krakow, 29 Listopada 46, 31-425 Krakow, Poland

autor

Socha J.

• Department of Biometry and Forest Productivity, University of Agriculture in Krakow, 29 Listopada 46, 31-425 Krakow, Poland

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Identyfikatory

DOI

10.1515/intag-2015-0009