High concentration of cadmium induces AtPCS2 gene expression in Arabidopsis thaliana (L.) Heynh ecotype Wassilewskija seedlings

• Autorzy: Moudouma C.F. , Gloaguen V. , Riou C. , Forestier L. , Saladin G.
• Języki publikacji: EN

Abstrakty

EN

In Arabidopsis thaliana, two genes encoding phytochelatin synthase (PCS; EC 2.3.2.15), AtPCS1 and AtPCS2, have been identified. Until now, only AtPCS1 was shown to play a role in response to Cd. To gain insight into the putative role of AtPCS2, three Cd concentrations (50, 100 and 200 µM) and long-term exposure (7 days) were tested on 1-week-old A. thaliana ecotype Wassilewskija (Ws) seedlings. Since 100 µM Cd did not alter seedling metabolism, as shown by unchanged total soluble protein and free proline contents, we investigated plantlet response to this concentration in addition to Cd accumulation. Seedlings accumulated Cd in roots and shoots. As phytochelatins and glutathione (GSH) contents increased in treated seedlings, we suggested that Cd might be translocated via the phytochelatin pathway. Specific enzymatic activities of γ-glutamylcysteine synthetase (GCS; EC 6.3.2.2), glutathione synthetase (GS; EC 6.3.2.3) and PCS were twice much more stimulated in shoots and roots after Cd exposure except GS that remained constant in shoots. As expression of genes encoding GCS and GS was unchanged in response to Cd, we suggested a regulation at translational or post-translational level. Surprisingly, AtPCS1 and AtPCS2 were differentially up-regulated after Cd treatment: AtPCS1 in shoots and AtPCS2 in whole plantlets. This last result suggests that PCS2 could be involved in plant response to high concentration of Cd in Ws ecotype and supports a putative role of PCS2, not redundant with PCS1, in a long-term response to Cd.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 3
• Opis fizyczny: p.1083-1091,fig.,ref.

Twórcy

autor

Moudouma C.F.

• Laboratorie de Chimie des Substances Naturelles (EA 1069), Faculte des Siences et Techniques, Universite de Limoges, 123 avenue Albert Thomas, 87060 Limoges Cedex, France

autor

Gloaguen V.

• Laboratorie de Chimie des Substances Naturelles (EA 1069), Faculte des Siences et Techniques, Universite de Limoges, 123 avenue Albert Thomas, 87060 Limoges Cedex, France

autor

Riou C.

• Laboratorie de Chimie des Substances Naturelles (EA 1069), Faculte des Siences et Techniques, Universite de Limoges, 123 avenue Albert Thomas, 87060 Limoges Cedex, France

autor

Forestier L.

• Unite de Genetique Moleculaire et Animale (UMR INRA 1061), Faculte des Sciences et Techniques, Universite de Limoges, 123 avenue Albert Thomas, 87060 Limoges Cedex, France

autor

Saladin G.

• Laboratorie de Chimie des Substances Naturelles (EA 1069), Faculte des Siences et Techniques, Universite de Limoges, 123 avenue Albert Thomas, 87060 Limoges Cedex, France

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