The effect of metamizole and tolfenamic acid on canine and equine adipose-derived mesenchymal stem cells (ASCs) an in vitro research

• Autorzy: Nicpon J. , Siudzinska A. , Maredziak M. , Smieszek A. , Basinska K. , Koszykowska M.
• Języki publikacji: EN

Abstrakty

EN

The influences of NSAIDs (Nonsteroidal Anti-inflammatory Drugs) - non-selective metamizole and selectively-acting tolfenamic acid were estimated on morphology, ultrastructure, and cytophysiological activity of canine (Ca) and equine (Eq) adipose-derived mesenchymal stem cells (ASCs). The lowest concentration of metamizole (0.01 mg/mL) stimulated the viability and cytophysiological activity of Ca ASCs and did not affect cell morphology. Stimulated cells possessed a proper, fibroblastic shape, with large, eccentrically located nuclei. Similar effects to those observed in Ca ASCs were found in Eq cells treated with both drugs. Cells cultivated with the intermediate (0.1 mg/mL) doses of NSAIDs displayed proper cell morphology, whereas cells cultivated in intermediate dose (0.1 mg/mL) became more flattened. The highest concentrations (1 mg/mL) of both drugs resulted in a cytotoxic effect in Ca and Eq ASCs. Based on these results, we conclude that stimulation of Ca and Eq ASCs with metamizole as well as Eq ASCs with tolfenamic acid can lead to positive effects only when the lowest drug concentrations are applied. This study indicates a different cellular response of canine and equine ASCs treated with metamizole and tolfenamic acid. The obtained data might be potentially useful in the study of functionalized veterinary biomaterials.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 1
• Opis fizyczny: p.3-11,ref.

Twórcy

autor

Nicpon J.

• Department and Clinic of Veterinary Surgery, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 51, 50-366 Wroclaw, Poland

autor

Siudzinska A.

• Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kozuchowska 5b, 50-631 Wroclaw, Poland

autor

Maredziak M.

• Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kozuchowska 5b, 50-631 Wroclaw, Poland

autor

Smieszek A.

• Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kozuchowska 5b, 50-631 Wroclaw, Poland
• Wroclaw Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland

autor

Basinska K.

• Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kozuchowska 5b, 50-631 Wroclaw, Poland

autor

Koszykowska M.

• Wroclaw Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland

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Identyfikatory

DOI

10.1515/pjvs-2015-000